Zobrazeno 1 - 10
of 192
pro vyhledávání: '"Wilfried A. Kues"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)
Abstract Maternal germ plasm determines the germline in birds. Previously, we proposed the chicken-specific Bucky ball (cBuc) as a functional equivalent of the zebrafish germ plasm organizer. This study demonstrated the maternal cBuc synthesis, and v
Externí odkaz:
https://doaj.org/article/11762c0d86ad4d94a03aae2087c10870
Publikováno v:
Signal Transduction and Targeted Therapy, Vol 9, Iss 1, Pp 1-2 (2024)
Externí odkaz:
https://doaj.org/article/d3aab262c8b14e5b918775ef523cb5ad
Autor:
Shahin Eghbalsaied, Wilfried A. Kues
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-15 (2023)
Abstract On-target integration of large cassettes via homology-directed repair (HDR) has several applications. However, the HDR-mediated targeted knock-in suffered from low efficiency. In this study, we made several large plasmids (12.1–13.4 kb) wh
Externí odkaz:
https://doaj.org/article/a0aa8b7c4b874f4293882b40ef567b71
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)
Abstract Bucky ball was identified as germ plasm organizer in zebrafish and has proven crucial for Balbiani body condensation. A synteny comparison identified an uncharacterized gene locus in the chicken genome as predicted avian counterpart. Here, w
Externí odkaz:
https://doaj.org/article/deae57f51282422491ea68d07bbcaeb7
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
Abstract Primordial germ cells (PGCs), the precursors of sperm and oocytes, pass on the genetic material to the next generation. The previously established culture system of chicken PGCs holds many possibilities for functional genomics studies and th
Externí odkaz:
https://doaj.org/article/731dca4c8633414c96d99218e8b58ce9
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
Abstract The chicken (Gallus gallus) is one of the most common and widespread domestic species, with an estimated total population of 25 billion birds worldwide. The vast majority of chickens in agriculture originate from hybrid breeding programs and
Externí odkaz:
https://doaj.org/article/f0989119e0d04542ba759247cf8881fd
Autor:
Wilfried A. Kues, Dharmendra Kumar
Publikováno v:
Signal Transduction and Targeted Therapy, Vol 7, Iss 1, Pp 1-2 (2022)
Externí odkaz:
https://doaj.org/article/e164e6425ab245bb8fdafbac7fd34fe1
Publikováno v:
BMC Molecular and Cell Biology, Vol 21, Iss 1, Pp 1-8 (2020)
Abstract Background Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulner
Externí odkaz:
https://doaj.org/article/0db1923dd82d451394e8ff683178496a
Autor:
Wilfried A. Kues
Publikováno v:
Signal Transduction and Targeted Therapy, Vol 6, Iss 1, Pp 1-2 (2021)
Externí odkaz:
https://doaj.org/article/afbbabbb33434145bbfde55bf95a5bb5
Autor:
Charis Drummer, Edgar-John Vogt, Michael Heistermann, Berit Roshani, Tamara Becker, Kerstin Mätz-Rensing, Wilfried A. Kues, Sebastian Kügler, Rüdiger Behr
Publikováno v:
Cells, Vol 10, Iss 3, p 505 (2021)
Genetic modification of non-human primates (NHP) paves the way for realistic disease models. The common marmoset is a NHP species increasingly used in biomedical research. Despite the invention of RNA-guided nucleases, one strategy for protein overex
Externí odkaz:
https://doaj.org/article/b948de6e32af4dd186330aa2da7719a4