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pro vyhledávání: '"Wilbur L. Whittle"'
Autor:
Robert T. Dell'Orco, Wilbur L. Whittle
Publikováno v:
Mechanisms of Ageing and Development. 15:141-152
Excision repair after ultraviolet (UV) irradiation was estimated in human diploid fibroblast-like cells (HDF) by treating DNA with a crude extract from Micrococcus luteus that contained pyrimidine dimer-specific endonucleases. The assays were done be
Autor:
Linda G. Green, Gary S. Stein, Janet L. Stein, Wilbur L. Whittle, Robert T. Dell'Orco, Harry Ostrer
Publikováno v:
Experimental Cell Research. 164:507-515
Analysis of the restriction enzyme digests of total genomic DNAs from a broad spectrum of human cell lines and from individuals with different genetic backgrounds, by hybridization with a series of cloned human histone sequences, indicated restrictio
Publikováno v:
In Vitro. 6:75-88
Within the entire spectrum of cell and tissue work to date, perfusion culture methods have received relatively little attention. Most of the perfusion systems have been designed for single monolayer or explant type cultures, and used principally for
Publikováno v:
The Journal of Cell Biology
Cell types in addition to those previously described (Kruse et al. 1963. J. Nat. Cancer Inst. 31:109; Kruse and Miedema. 1965. J. Cell Biol. 27:273) were found to form multiple-layered cultures by perfusion-culture technique. Dense populations contai
Publikováno v:
Mechanisms of ageing and development. 36(3)
The extent to which human histone gene organization is conserved during the in vitro lifespan of human diploid fibroblast-like cells was determined by comparing the restriction patterns of a human H4 and an H3 histone gene from cells of various in vi
Autor:
Wilbur L. Whittle, Robert T. Dell'Orco
Publikováno v:
Mechanisms of ageing and development. 8(4)
Unscheduled DNA synthesis, used as a measure of excision repair following exposure to ultra-violet irradiation, was determined in confluent and arrested human diploid fibroblasts and correlated with in vitro age. Confluent cultures exhibited identica