Identification of storage conditions stabilizing extracellular vesicles pre

Autor: Görgens, A, Corso, G, Hagey, DW, Jawad Wiklander, R, Gustafsson, MO, Felldin, U, Lee, Y, Bostancioglu, RB, Sork, H, Liang, X, Zheng, W, Mohammad, DK, Van de Wakker, SI, Vader, P, Zickler, AM, Mamand, DR, Ma, L, Holme, MN, Stevens, MM, Wiklander, OPB, El Andaloussi, S

Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological processes and hold great potential for therapeutic and diagnostic use. Despite significant advances within the last decade, the key issue of EV storage stabilit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1032::510316ad9f188eacc885071085b1d4c3
http://hdl.handle.net/10044/1/97850

Novel Endogenous Engineering Platform for Robust Loading and Delivery of Functional mRNA by Extracellular Vesicles.

Autor: Zickler AM; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden., Liang X; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Cancer Research Laboratory, Shandong University-Karolinska Institutet collaborative Laboratory, School of Basic Medical Science, Shandong University, No. 44, Wenhua Xi Road, Ji'nan, Shandong, 250012, P. R. China., Gupta D; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Institute of Developmental and Regenerative Medicine, Department of Paediatrics., University of Oxford, Old Road Campus, Roosevelt Dr, Headington, Oxford, OX3 7TY, UK., Mamand DR; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Breast Center, Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, 14186, Sweden., De Luca M; Evox Therapeutics Ltd., Oxford Science Park, Medawar Centre, Robert Robinson Avenue, Oxford, OX4 4HG, UK.; Human Technopole, Viale Rita Levi Montalcini, 1, Milan, 20157, Italy., Corso G; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Evercyte GmbH, Leberstrasse 20, Vienna, 1110, Austria., Errichelli L; Evox Therapeutics Ltd., Oxford Science Park, Medawar Centre, Robert Robinson Avenue, Oxford, OX4 4HG, UK., Hean J; Evox Therapeutics Ltd., Oxford Science Park, Medawar Centre, Robert Robinson Avenue, Oxford, OX4 4HG, UK., Sen T; Evox Therapeutics Ltd., Oxford Science Park, Medawar Centre, Robert Robinson Avenue, Oxford, OX4 4HG, UK.; Lonza Biologics, Chesterford Research Park, Cambridge, CB10 1XL, UK., Elsharkasy OM; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden., Kamei N; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Laboratory of Drug Delivery Systems, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe, Hyogo, 650-8586, Japan., Niu Z; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324, Five Jing Road, Ji'nan, Shandong, 250012, P. R. China., Zhou G; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Department of Gynecology, The Obstetrics and Gynecology Hospital of Fudan University, No. 419, Fangxie Road, Shanghai, 200011, P. R. China., Zhou H; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden., Roudi S; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden., Wiklander OPB; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Breast Center, Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, 14186, Sweden., Görgens A; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany., Nordin JZ; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Clinical Immunology and Transfusion Medicine (KITM), Karolinska University Hospital, Stockholm, 14186, Sweden., Castilla-Llorente V; Evox Therapeutics Ltd., Oxford Science Park, Medawar Centre, Robert Robinson Avenue, Oxford, OX4 4HG, UK.; Uncommon Bio, Cambridge Technopark, Newmarket Rd, Cambridge, CB5 8PB, UK., El Andaloussi S; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Stockholm, 14186, Sweden.; Karolinska ATMP Center, Karolinska Institutet, ANA Futura, Alfred-Nobels-Allé 8, Huddinge, Stockholm, 14152, Sweden.

Publikováno v: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Sep 09, pp. e2407619. Date of Electronic Publication: 2024 Sep 09.

A protocol to differentiate the chondrogenic ATDC5 cell-line for the collection of chondrocyte-derived extracellular vesicles.

Autor: Marchan-Alvarez JG; Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden.; Astrid Lindgren Children's hospital Stockholm Sweden., Teeuwen L; Division of Immunology and Allergy, Department of Medicine (Solna) Karolinska Institutet Stockholm Sweden.; Clinical Immunology and Transfusion Medicine Karolinska University Hospital Stockholm Sweden., Mamand DR; Department of Laboratory Medicine Unit for Biomolecular and Cellular Medicine Karolinska Institutet Stockholm Sweden., Gabrielsson S; Division of Immunology and Allergy, Department of Medicine (Solna) Karolinska Institutet Stockholm Sweden.; Clinical Immunology and Transfusion Medicine Karolinska University Hospital Stockholm Sweden., Blomgren K; Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden.; Pediatric Oncology Karolinska University Hospital Stockholm Sweden., Wiklander OPB; Department of Laboratory Medicine Unit for Biomolecular and Cellular Medicine Karolinska Institutet Stockholm Sweden., Newton PT; Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden.; Astrid Lindgren Children's hospital Stockholm Sweden.

Publikováno v: Journal of extracellular biology [J Extracell Biol] 2024 Sep 05; Vol. 3 (9), pp. e70004. Date of Electronic Publication: 2024 Sep 05 (Print Publication: 2024).

Antibody-displaying extracellular vesicles for targeted cancer therapy.

Autor: Wiklander OPB; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden. oscar.wiklander@ki.se.; Breast Center, Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, Sweden. oscar.wiklander@ki.se.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden. oscar.wiklander@ki.se., Mamand DR; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Breast Center, Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden., Mohammad DK; Department of Medicine Huddinge, Karolinska Institute, Stockholm, Sweden.; College of Agricultural Engineering Sciences, Salahaddin University-Erbil, Erbil, Iraq., Zheng W; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Jawad Wiklander R; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Sych T; Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden., Zickler AM; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Liang X; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Sharma H; Evox Therapeutics Limited, Oxford, UK., Lavado A; Evox Therapeutics Limited, Oxford, UK., Bost J; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Roudi S; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Corso G; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Lennaárd AJ; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Abedi-Valugerdi M; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden., Mäger I; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Alici E; Department of Medicine Huddinge, Karolinska Institute, Stockholm, Sweden.; Hematology Center, Karolinska University Hospital, Stockholm, Sweden., Sezgin E; Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden., Nordin JZ; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden.; Department of Clinical Immunology and Transfusion Medicine (KITM), Karolinska University Hospital, Stockholm, Sweden., Gupta D; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden.; Department of Paediatrics, University of Oxford, Oxford, UK., Görgens A; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden.; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Duisburg, Germany., El Andaloussi S; Department of Laboratory Medicine, Unit for Biomolecular and Cellular Medicine, Karolinska Institutet, Stockholm, Sweden. samir.el-andaloussi@ki.se.; Karolinska ATMP Center, ANA Futura, Huddinge, Sweden. samir.el-andaloussi@ki.se.; Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden. samir.el-andaloussi@ki.se.

Publikováno v: Nature biomedical engineering [Nat Biomed Eng] 2024 May 20. Date of Electronic Publication: 2024 May 20.

Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles.

Autor: Zheng W; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Rädler J; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Sork H; Institute of Technology, University of Tartu, Tartu, Estonia., Niu Z; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.; Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China., Roudi S; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Bost JP; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Görgens A; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Zhao Y; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.; Clinical Research Center, Karolinska University Hospital, Stockholm, Sweden., Mamand DR; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Liang X; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Wiklander OPB; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Lehto T; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.; Institute of Technology, University of Tartu, Tartu, Estonia., Gupta D; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., Nordin JZ; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden., El Andaloussi S; Division of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden. samir.el-andaloussi@ki.se.

Publikováno v: Nature communications [Nat Commun] 2023 Aug 07; Vol. 14 (1), pp. 4734. Date of Electronic Publication: 2023 Aug 07.