Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Wieslaw Stryjewski"'
Autor:
Steven A. Soper, Michelle Galloway, Mateusz L. Hupert, Jost Goettert, Hamed Shadpour, Changgeng Liu, Donald Patterson, Wieslaw Stryjewski
Publikováno v:
Analytical Chemistry. 79:870-878
A 16-channel microfluidic chip with an integrated contact conductivity sensor array is presented. The microfluidic network consisted of 16 separation channels that were hot-embossed into polycarbonate (PC) using a high-precision micromilled metal mas
Publikováno v:
Analytical Biochemistry. 330:206-218
We examined the feasibility of using a two-color time-resolved detection scheme with microdevices for DNA sequencing applications. A home-built dual-color optical-fiber-based time-resolved near-infrared (IR) fluorescence microscope successfully coupl
Autor:
Musundi B. Wabuyele, Hannah Farquar, Yu-Wei Cheng, Francis Barany, Robert P. Hammer, Wieslaw Stryjewski, Steven A. Soper
Publikováno v:
Journal of the American Chemical Society. 125:6937-6945
The aim of this study was to develop new strategies for analyzing molecular signatures of disease states approaching real-time using single pair fluorescence resonance energy transfer (spFRET) to rapidly detect point mutations in unamplified genomic
Autor:
Yu-Wei Cheng, Bikas Vaidya, Wieslaw Stryjewski, Yun Wang, Francis Barany, Robin L. McCarley, Robert P. Hammer, Steven A. Soper, Hannah D. Farquar
Publikováno v:
Analytical Chemistry. 75:1130-1140
Low-density arrays were assembled into microfluidic channels hot-embossed in poly(methyl methacrylate) (PMMA) to allow the detection of low-abundant mutations in gene fragments (K-ras) that carry point mutations with high diagnostic value for colorec
Publikováno v:
ELECTROPHORESIS. 22:3939-3948
Single photon burst techniques were used to detect double-stranded DNA molecules in poly(methylmethacrylate) (PMMA) and polycarbonate (PC) microfluidic devices. A confocal epi-illumination detection system was constructed to monitor the fluorescence
Publikováno v:
Journal of Colloid and Interface Science. 239:374-379
Fluorescence photobleaching recovery, long a staple tool of complex fluid experimentation, is discussed as a method for size characterization of colloidal particles. Specific comparisons are made to dynamic light scattering, which is often used for s
Autor:
Rex Peterson, Lyle R. Middendorf, John A. Wurm, Steven A. Soper, Benjamin L. Legendre, Wieslaw Stryjewski, Suzanne J. Lassiter, Rainer Erdmann, Michael Wahl
Publikováno v:
Analytical Chemistry. 72:5373-5382
A compact time-resolved near-IR fluorescence imager was constructed to obtain lifetime and intensity images of DNA sequencing slab gels. The scanner consisted of a microscope body with f/1.2 relay optics onto which was mounted a pulsed diode laser (r
Autor:
Elena Temyanko, Debbie L. Tipton, Garrett Doucet, Wieslaw Stryjewski, Paul S. Russo, Michael Baylis, Zimei Bu
Publikováno v:
The Journal of Chemical Physics. 111:1746-1752
The self-diffusion of fluorescently tagged poly(γ-benzyl-α,L-glutamate), a helical, semiflexible synthetic homopolypeptide, has been measured in isotropic and cholesteric liquid-crystalline solutions by pattern fluorescence photobleaching recovery.
Publikováno v:
Review of Scientific Instruments. 70:32-37
A fiber-optic-based multichannel time-correlated single-photon-counting device with subnanosecond time resolution was developed. A passively mode-locked Ti:sapphire laser provided the excitation source for 12 separate time-correlated single-photon co
Autor:
Wieslaw Stryjewski, Mary D. Barkley, Fahmida N. Chowdhury, Paul S. Russo, Soon-Jong Kim, E. S. Younathan
Publikováno v:
Biophysical Journal. 65(1):215-226
The fluorescence of the single tryptophan in Bacillus stearothermophilus phosphofructokinase was characterized by steady-state and time-resolved techniques. The enzyme is a tetramer of identical subunits, which undergo a concerted allosteric transiti