Zobrazeno 1 - 10
of 60
pro vyhledávání: '"Wieslaw Kudlicki"'
Publikováno v:
The Scientific World Journal, Vol 2, Pp 7-8 (2002)
Externí odkaz:
https://doaj.org/article/e94958129cf24632bf909f0d2b38153c
Autor:
Federico Katzen, Julia E. Fletcher, Jian-Ping Yang, Sanjay Vasu, Todd Peterson, Wieslaw Kudlicki
Publikováno v:
BioTechniques, Vol 45, Iss 5, p 587 (2008)
Externí odkaz:
https://doaj.org/article/186a7918c3204f8291cd05145b936ea4
Autor:
Matthew A. Coleman, Federico Katzen, Sonny Ly, Craig D. Blanchette, Thomas R Huser, Feliza Bourguet, Wieslaw Kudlicki, Ted A. Laurence, Tingjuan Gao, Wei He, Paul T. Henderson
Publikováno v:
Protein Science. 20:437-447
Nanolipoprotein particles (NLPs) represent a unique nanometer-sized scaffold for supporting membrane proteins (MP). Characterization of their dynamic shape and association with MP in solution remains a challenge. Here, we present a rapid method of an
Publikováno v:
Trends in Biotechnology. 27:455-460
Structural and functional studies of membrane proteins have been severely hampered by difficulties in producing sufficient quantities of properly folded protein products. It is well established that cell-based expression of membrane proteins is gener
Autor:
Todd Peterson, Paul D. Hoeprich, Wieslaw Kudlicki, Kenneth J. Rothschild, Matthew A. Coleman, Federico Katzen, Craig D. Blanchette, Graham Bench, Brett A. Chromy, Angela K. Hinz, Jenny A. Cappuccio, Edward A. Kuhn, Brent W. Segelke, Erin S. Arroyo, Joel M. Kralj, Todd Sulchek, Julia Fletcher
Publikováno v:
Molecular & Cellular Proteomics. 7:2246-2253
Here we demonstrate rapid production of solubilized and functional membrane protein by simultaneous cell-free expression of an apolipoprotein and a membrane protein in the presence of lipids, leading to the self-assembly of membrane protein-containin
Autor:
Federico Katzen, Wieslaw Kudlicki
Publikováno v:
Journal of Biotechnology. 125:194-197
A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processin
Autor:
Boyd Hardesty, Donald Simmons, Wieslaw Kudlicki, Tamara Tsalkova, Diane McCarthy, Gisela Kramer
Publikováno v:
The International Journal of Biochemistry & Cell Biology. 31:231-241
Important aspects of translation are release and folding of the synthesized protein into its three-dimensional structure. Studies from our group indicated that during in vitro protein synthesis a large portion of full-length polypeptides apparently a
Publikováno v:
Journal of Biological Chemistry. 272:32206-32210
The translation elongation factor (EF) Tu has chaperone-like capacity to promote renaturation of denatured rhodanese. This renaturation activity is greatly increased under conditions in which the factor can oscillate between the open and closed confo
Publikováno v:
Analytical Biochemistry. 245:249-252
Publikováno v:
Biochemistry and Cell Biology. 73:1199-1207
Evidence is presented for cotranslational folding of rhodanese or ricin during its synthesis on Escherichia coli ribosomes. During transcription–translation, full-length but enzymatically inactive polypeptides accumulated as peptidyl-tRNA on the ri