Template-independent enzymatic synthesis of RNA oligonucleotides.

Autor: Wiegand DJ; Department of Genetics, Harvard Medical School, Boston, MA, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA.; EnPlusOne Biosciences Inc., Watertown, MA, USA., Rittichier J; Department of Genetics, Harvard Medical School, Boston, MA, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA.; EnPlusOne Biosciences Inc., Watertown, MA, USA., Meyer E; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA.; EnPlusOne Biosciences Inc., Watertown, MA, USA., Lee H; Department of Genetics, Harvard Medical School, Boston, MA, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA., Conway NJ; Department of Genetics, Harvard Medical School, Boston, MA, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA., Ahlstedt D; EnPlusOne Biosciences Inc., Watertown, MA, USA., Yurtsever Z; EnPlusOne Biosciences Inc., Watertown, MA, USA., Rainone D; EnPlusOne Biosciences Inc., Watertown, MA, USA., Kuru E; Department of Genetics, Harvard Medical School, Boston, MA, USA. erkin_kuru@hms.harvard.edu.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA. erkin_kuru@hms.harvard.edu., Church GM; Department of Genetics, Harvard Medical School, Boston, MA, USA. gchurch@genetics.med.harvard.edu.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA. gchurch@genetics.med.harvard.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2024 Jul 12. Date of Electronic Publication: 2024 Jul 12.

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens.

Autor: Wiegand DJ; Department of Genetics, Harvard Medical School; Wyss Institute for Biologically Inspired Engineering; Daniel.Wiegand@wyss.harvard.edu., Lee HH; Department of Genetics, Harvard Medical School., Ostrov N; Department of Genetics, Harvard Medical School; nili_ostrov@hms.harvard.edu., Church GM; Department of Genetics, Harvard Medical School; Wyss Institute for Biologically Inspired Engineering; gchurch@genetics.med.harvard.edu.

Publikováno v: Journal of visualized experiments : JoVE [J Vis Exp] 2019 Mar 14 (145). Date of Electronic Publication: 2019 Mar 14.

Highly efficient Cas9-mediated transcriptional programming.

Autor: Chavez A; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA., Scheiman J; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA., Vora S; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. [3] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Pruitt BW; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA., Tuttle M; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA., P R Iyer E; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA., Lin S; 1] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. [2] Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA. [3] School of Life Sciences, Tsinghua University, Beijing, China., Kiani S; 1] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Guzman CD; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA., Wiegand DJ; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA., Ter-Ovanesyan D; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA., Braff JL; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA., Davidsohn N; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA., Housden BE; 1] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. [2] Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA., Perrimon N; 1] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. [2] Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA., Weiss R; 1] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Aach J; Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA., Collins JJ; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Institute for Medical Engineering &Science, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [4] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Church GM; 1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

Publikováno v: Nature methods [Nat Methods] 2015 Apr; Vol. 12 (4), pp. 326-8. Date of Electronic Publication: 2015 Mar 02.