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Proteomic analysis of the cell cycle of procylic form Trypanosoma brucei

Autor: Crozier, TWM, Tinti, M, Wheeler, RJ, Ly, T, Ferguson, MAJ, Lamond, AI

Publikováno v: Crozier, T W M, Tinti, M, Wheeler, R J, Ly, T, Ferguson, M A J & Lamond, A I 2018, ' Proteomic analysis of the cell cycle of procylic form Trypanosoma brucei ', Molecular and Cellular Proteomics, vol. 17, no. 6, RA118.000650, pp. 1184-1195 . https://doi.org/10.1074/mcp.RA118.000650
Molecular & Cellular Proteomics : MCP

We describe a single-step centrifugal elutriation method to produce synchronous Gap1 (G1)-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag (TMT) labeling and mass spectrometry (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::a42a5460527d6709af487dd181b5581b
https://www.pure.ed.ac.uk/ws/files/64287282/Proteomic_Analysis_PUB_OA_CC_BY_Mol_Cell_Proteomics_2018_Crozier_1184_95.pdf

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TrypTag.org – Genome wide protein localisation reveals organelle sub‐domains, with functional consequences in the flagellum

Autor: Sunter, JD, Wheeler, RJ, Dean, S, Edwards, B, Gull, K

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1064::db3e41fb35e43356321a3955445b7109
https://ora.ox.ac.uk/objects/uuid:f1a0c6fe-d4d5-49e4-9761-c7b63538dee6

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Controlling compartmentalization by non-membrane-bound organelles

Autor: Wheeler, RJ, Hyman, AA

Publikováno v: Philosophical Transactions of the Royal Society B: Biological Sciences

Compartmentalization is a characterizing feature of complexity in cells, used to organize their biochemistry. Membrane-bound organelles are most widely known, but non-membrane-bound liquid organelles also exist. These have recently been shown to form

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::fc5e2e808e2ec0708eb66b75552cdc6e
http://europepmc.org/articles/PMC5904305

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Extensive flagellar remodeling during the complex life cycle of &ITParatrypanosoma&IT, an early-branching trypanosomatid

Autor: Skalicky, T, Dobakova, E, Wheeler, RJ, Tesarova, M, Flegontov, P, Jirsova, D, Votypka, J, Yurchenko, V, Ayala, FJ, Lukes, J

Publikováno v: Skalicky, T; Dobakova, E; Wheeler, RJ; Tesarova, M; Flegontov, P; Jirsova, D; et al.(2017). Extensive flagellar remodeling during the complex life cycle of &ITParatrypanosoma&IT, an early-branching trypanosomatid. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(44), 11757-11762. doi: 10.1073/pnas.1712311114. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/6rc7w7h2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::09b8eab291a1186bf3c3f672849564e7
http://www.escholarship.org/uc/item/6rc7w7h2

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Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone

Autor: Wheeler, RJ, Sunter, JD, Gull, K

Publikováno v: Journal of Cell Science. 129(4)

Leishmania promastigote parasites have a flagellum, which protrudes from the flagellar pocket at the cell anterior, yet, surprisingly, have homologs of many flagellum attachment zone (FAZ) proteins - proteins used in the related Trypanosoma species t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1064::8528a6660e43c18eb57835ec12c814f9
http://ora.ox.ac.uk/objects/uuid:5a43a565-897d-4356-bbf9-cae7207c49d5

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