Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions.

Autor: Butler D; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Mozsary C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Meydan C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.; WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY, USA., Foox J; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA., Rosiene J; New York Genome Center, New York, NY, USA.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Shaiber A; New York Genome Center, New York, NY, USA.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA.; Englander Institute for Precision Medicine and the Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA., Danko D; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Afshinnekoo E; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.; WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY, USA., MacKay M; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Sedlazeck FJ; Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA., Ivanov NA; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.; Clinical & Translational Science Center, Weill Cornell Medicine, New York, NY, USA., Sierra M; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA., Pohle D; Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital Tuebingen, Tuebingen, Germany., Zietz M; Department of Biomedical Informatics, Department of Systems Biology, Department of Medicine, Institute for Genomic Medicine, Columbia University, Columbia, NY, USA., Gisladottir U; Department of Biomedical Informatics, Department of Systems Biology, Department of Medicine, Institute for Genomic Medicine, Columbia University, Columbia, NY, USA., Ramlall V; Department of Biomedical Informatics, Department of Systems Biology, Department of Medicine, Institute for Genomic Medicine, Columbia University, Columbia, NY, USA.; Department of Cellular, Molecular Physiology & Biophysics, Columbia University, Columbia, NY, USA., Sholle ET; Information Technologies & Services Department, Weill Cornell Medicine, New York, NY, USA., Schenck EJ; Department of Medicine, Weill Cornell Medicine, New York, NY, USA., Westover CD; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Hassan C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Ryon K; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Young B; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Bhattacharya C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Ng DL; Department of Laboratory Medicine, University of California, San Francisco, CA, USA., Granados AC; Department of Laboratory Medicine, University of California, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Santos YA; Department of Laboratory Medicine, University of California, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Servellita V; Department of Laboratory Medicine, University of California, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Federman S; Department of Laboratory Medicine, University of California, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Ruggiero P; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Fungtammasan A; DNAnexus, Inc., Mountain View, CA, USA., Chin CS; DNAnexus, Inc., Mountain View, CA, USA., Pearson NM; Root Deep Insight, Boston, MA, USA., Langhorst BW; New England Biolabs, Ipswich, MA, USA., Tanner NA; New England Biolabs, Ipswich, MA, USA., Kim Y; NanoString Technologies, Seattle, WA, USA., Reeves JW; NanoString Technologies, Seattle, WA, USA., Hether TD; NanoString Technologies, Seattle, WA, USA., Warren SE; NanoString Technologies, Seattle, WA, USA., Bailey M; NanoString Technologies, Seattle, WA, USA., Gawrys J; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Meleshko D; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; Tri-Institutional Computational Biology & Medicine Program, Weill Cornell Medicine, New York, NY, USA., Xu D; Genomics Resources Core Facility, Weill Cornell Medicine, New York, NY, USA., Couto-Rodriguez M; Biotia, Inc., New York, NY, USA., Nagy-Szakal D; Biotia, Inc., New York, NY, USA.; Department of Cell Biology, SUNY Downstate Health Sciences University, New York, NY, USA., Barrows J; Biotia, Inc., New York, NY, USA., Wells H; Biotia, Inc., New York, NY, USA., O'Hara NB; Biotia, Inc., New York, NY, USA.; Department of Cell Biology, SUNY Downstate Health Sciences University, New York, NY, USA., Rosenfeld JA; Rutgers Cancer Institute of New Jersey, New York, NJ, USA.; Department of Pathology, Robert Wood Johnson Medical School, New York, NJ, USA., Chen Y; Rutgers Cancer Institute of New Jersey, New York, NJ, USA., Steel PAD; Department of Emergency Medicine, Weill Cornell Medicine, New York, NY, USA., Shemesh AJ; Department of Emergency Medicine, Weill Cornell Medicine, New York, NY, USA., Xiang J; Genomics Resources Core Facility, Weill Cornell Medicine, New York, NY, USA., Thierry-Mieg J; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Thierry-Mieg D; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Iftner A; Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital Tuebingen, Tuebingen, Germany., Bezdan D; Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital Tuebingen, Tuebingen, Germany., Sanchez E; Department of Medicine, Weill Cornell Medicine, New York, NY, USA., Campion TR Jr; Information Technologies & Services Department, Weill Cornell Medicine, New York, NY, USA.; Department of Population Health Sciences, Weill Cornell Medicine, New York, NY, USA., Sipley J; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Cong L; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Craney A; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Velu P; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Melnick AM; Department of Medicine, Weill Cornell Medicine, New York, NY, USA., Shapira S; Department of Biomedical Informatics, Department of Systems Biology, Department of Medicine, Institute for Genomic Medicine, Columbia University, Columbia, NY, USA., Hajirasouliha I; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.; Englander Institute for Precision Medicine and the Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA., Borczuk A; Department of Medicine, Weill Cornell Medicine, New York, NY, USA., Iftner T; Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital Tuebingen, Tuebingen, Germany., Salvatore M; Department of Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Population Health Sciences, Weill Cornell Medicine, New York, NY, USA., Loda M; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Westblade LF; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY, USA., Cushing M; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Wu S; Hangzhou Cancer Institute, Hangzhou Cancer Hospital, Hangzhou, China.; Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou, China., Levy S; HudsonAlpha Discovery Institute, Huntsville, AL, USA., Chiu C; Department of Laboratory Medicine, University of California, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA.; Department of Medicine, Division of Infectious Diseases, University of California, San Francisco, CA, USA., Schwartz RE; Department of Medicine, Weill Cornell Medicine, New York, NY, USA. res2025@med.cornell.edu., Tatonetti N; Department of Biomedical Informatics, Department of Systems Biology, Department of Medicine, Institute for Genomic Medicine, Columbia University, Columbia, NY, USA. npt2105@cumc.columbia.edu., Rennert H; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA. har2006@med.cornell.edu., Imielinski M; New York Genome Center, New York, NY, USA. mai9037@med.cornell.edu.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA. mai9037@med.cornell.edu.; Englander Institute for Precision Medicine and the Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA. mai9037@med.cornell.edu., Mason CE; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.; WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.; The Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.

Publikováno v: Nature communications [Nat Commun] 2021 Mar 12; Vol. 12 (1), pp. 1660. Date of Electronic Publication: 2021 Mar 12.

C-terminal amidation of PACAP-38 and PACAP-27 is dispensable for biological activity at the PAC1 receptor.

Autor: Emery AC; Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA., Alvarez RA; Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA., Abboud P; Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA., Xu W; Section on Directed Gene Transfer, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA., Westover CD; Section on Directed Gene Transfer, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA., Eiden MV; Section on Directed Gene Transfer, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA., Eiden LE; Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health, Bethesda, MD 20892, USA. Electronic address: eidenl@mail.nih.gov.

Publikováno v: Peptides [Peptides] 2016 May; Vol. 79, pp. 39-48. Date of Electronic Publication: 2016 Mar 11.