Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Wesley E. Stites"'
Publikováno v:
Biophysical Chemistry. :86-94
Six single substitution mutations, V66F, V66G, V66N, V66Q, V66S, V66T, and V66Y, were made in the background of a highly stable triple mutant (P117G, H124L, and S128A) of staphylococcal nuclease. The thermodynamic stabilities of wild type staphylococ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fed08de79e5e08e67e0ac866cd79bef
https://doi.org/10.1016/j.bpc.2013.06.018
https://doi.org/10.1016/j.bpc.2013.06.018
Autor:
NhatHai Phan, Latisha M. Puckett, Wesley E. Stites, Derek B. Pyland, Joel A. Starch, Rohana Liyanage, Mark R. Okimoto, Jackson O. Lay, Nagarjuna Devarapalli, Jennifer Gidden
Publikováno v:
International Journal of Mass Spectrometry. :63-70
Protein equilibrium snapshot by hydrogen/deuterium exchange electrospray ionization mass spectrometry (PEPS-HDX-ESI-MS or PEPS) is a method recently introduced for estimating protein folding energies and rates. Herein we describe the basis for this m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::013d924c836bf384f30bf17256fb4316
https://doi.org/10.1016/j.ijms.2012.10.010
https://doi.org/10.1016/j.ijms.2012.10.010
Publikováno v:
Biophysical Journal. 112:48a-49a
Background: High level of protein carbonyls have been found in plasma proteins in smokers compared to non-smokers. While oxidation may directly interfere with activity, the extent to which oxidation affects protein turnover is less clear.Objectives:
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eccfcae4c4dc2f89d81bff3697bd2059
https://doi.org/10.1016/j.bpj.2016.11.301
https://doi.org/10.1016/j.bpj.2016.11.301
Autor:
Yun Ho Kim, Wesley E. Stites
Publikováno v:
Biochemistry. 47:8804-8814
To explore the effects of molecular crowding and excluded volume upon protein stability, we used a series of cross-linking reagents with nine different single-cysteine mutants of staphylococcal nuclease to make covalently linked dimers. These cross-l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1da5c2c17d67528b46202833a2398a87
https://doi.org/10.1021/bi800297j
https://doi.org/10.1021/bi800297j
Autor:
Wesley E. Stites, Deepika Talla-Singh
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 71:1607-1616
The change in heat capacity, DeltaC(p), on protein unfolding has been usually determined by calorimetry. A noncalorimetric method which employs the Gibbs-Helmholtz relationship to determine DeltaC(p) has seen some use. Generally, in this method the f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0af99ecfe90ab4c263ba0eadeae669c
https://doi.org/10.1002/prot.22016
https://doi.org/10.1002/prot.22016
Autor:
Martin Hauer-Jensen, Louis M. Fink, Wesley E. Stites, Jason Plaxco, Junru Wang, Stewart L. MacLeod, Jeffrey W. Froude, Christopher C. Ross
Publikováno v:
Radiation Research. 169:408-416
Normal tissue radiation injury is associated with loss of vascular thromboresistance, notably because of deficient levels of endothelial thrombomodulin (TM). TM is located on the luminal surface of most endothelial cells and has critical anticoagulan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e069f8ab6166f35ba8ebc9a50e6e84a
https://doi.org/10.1667/rr1148.1
https://doi.org/10.1667/rr1148.1
Autor:
Wesley E. Stites, Michael P. Byrne
Publikováno v:
Biophysical Chemistry. 125:490-496
It is unclear whether the thermal denaturation of staphylococcal nuclease is a two state, three state, or variable two state process. The thermal denaturation of wild-type staphylococcal nuclease was followed by tryptophan fluorescence and circular d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47771cbcf7a13e353bc868110891a21b
https://doi.org/10.1016/j.bpc.2006.10.014
https://doi.org/10.1016/j.bpc.2006.10.014
Autor:
Jeffrey W. Froude, Wesley E. Stites
Publikováno v:
Medical Hypotheses. 68:811-821
The leading cause of premature death in smokers is cardiovascular disease. Diabetics also suffer from increased cardiovascular disease. This results, in part, from the hypercoagulable state associated with these conditions. However, the molecular cau
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::742172e64eccce1f1c51c5508c52958f
https://doi.org/10.1016/j.mehy.2006.09.009
https://doi.org/10.1016/j.mehy.2006.09.009
Publikováno v:
Biophysical Chemistry. 110:239-248
Efforts to design proteins with greatly reduced sequence diversity have often resulted in proteins with so-called molten globule properties. Substitutions were made at six neighboring sites in the major hydrophobic core of staphylococcal nuclease to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a32cbb5c8df0176366e20cc35229703
https://doi.org/10.1016/j.bpc.2004.02.007
https://doi.org/10.1016/j.bpc.2004.02.007
Publikováno v:
Analytical Biochemistry. 421:767-769
Study of the posttranslational modification of methionine to its sulfoxide has been receiving increasing attention because of its implication in regulation of protein activity, but techniques for the detection of this modification remain limited. In
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e222f93e7fb0445228f3ea1794fbd71
https://doi.org/10.1016/j.ab.2011.12.021
https://doi.org/10.1016/j.ab.2011.12.021