A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways

Autor: Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas

SummaryChloroplasts are eukaryotic photosynthetic organelles that drive the global carbon cycle. Despite their importance, our understanding of their protein composition, function, and spatial organization remains limited. Here, we determined the loc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::71a4b15df616257d1c043e8d9b1dbb3d
https://doi.org/10.1101/2022.05.31.493820

A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii

Autor: Alan K. Itakura, Leif J. Pallesen, Lianyong Wang, Oliver D Caspari, Robyn Roth, Weronika Patena, Alistair J. McCormick, Howard Griffiths, Kher Xing Chan, Ursula Goodenough, Nicky Atkinson, Martin C. Jonikas, Gregory Reeves

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Itakura, A K, Chan, K X, Atkinson, N, Pallesen, L, Wang, L, Reeves, G, Patena, W, Caspari, O, Roth, R, Goodenough, U, McCormick, A J, Griffiths, H & Jonikas, M C 2019, ' A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii ', Proceedings of the National Academy of Sciences, vol. 116, no. 37, pp. 18445-18454 . https://doi.org/10.1073/pnas.1904587116

Significance Many cellular structures are assembled via phase separation, forming liquid-like droplets in a manner analogous to oil in water. How can the cell control whether there is 1 droplet or many? Here, we provide insights into this question by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7be7ff60b6ff73a5ca5f126d6bd9757d
https://doi.org/10.1073/pnas.1904587116

Systematic characterization of gene function in a photosynthetic organism

Autor: Arthur R. Grossman, Nemeth M, Salomé Pa, Silvia Ramundo, Sean R. Cutler, Rick G. Kim, Weronika Patena, Philp C, Krishna K. Niyogi, Dinneny, Friedrich Fauser, Josep Vilarrasa-Blasi, Sabeeha S. Merchant, Masayuki Onishi, Setsuko Wakao, Xiaoxiao Li, Osaki J, Millican M, Robert E. Jinkerson, Peter Walter, Martin C. Jonikas, Kaye Y

Photosynthetic organisms are essential for human life, yet most of their genes remain functionally uncharacterized. Single-celled photosynthetic model systems have the potential to accelerate our ability to connect genes to functions. Here, using a b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c6169bda405a0f900f9e5dafdc47211a
https://doi.org/10.1101/2020.12.11.420950

Assembly of the algal CO2-fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif

Autor: Chun S. Lau, Alan K. Itakura, Lianyong Wang, Weronika Patena, Gary Yates, Martin C. Jonikas, Tom Z. Emrich-Mills, Luke C. M. Mackinder, Moritz T. Meyer, Shan He

Approximately one-third of the Earth’s photosynthetic CO2 assimilation occurs in a pyrenoid, an organelle containing the CO2-fixing enzyme Rubisco. How constituent proteins are recruited to the pyrenoid, and how the organelle’s sub-compartments -

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c643e3596077e6b2e5928dca64f1f1f7
https://doi.org/10.1101/2020.08.16.252858

Assembly of the algal CO

Autor: Moritz T, Meyer, Alan K, Itakura, Weronika, Patena, Lianyong, Wang, Shan, He, Tom, Emrich-Mills, Chun S, Lau, Gary, Yates, Luke C M, Mackinder, Martin C, Jonikas

Publikováno v: Science Advances

A protein motif mediates targeting of proteins to the pyrenoid and appears to link the pyrenoid’s three subcompartments.
Approximately one-third of the Earth’s photosynthetic CO2 assimilation occurs in a pyrenoid, an organelle containing the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4ea96fe3279b307a1707eabaa5c17728
https://pubmed.ncbi.nlm.nih.gov/33177094