Proteome analysis of heat shock protein expression in Bradyrhizobium japonicum

Autor: Paola Dainese, Peter James, Franz Narberhaus, Martin Münchbach, Werner Staudenmann

Publikováno v: European Journal of Biochemistry. 264:39-48

A set of 19 heat shock proteins (Hsp) was observed - by subtractive two-dimensional gel electrophoresis - to be induced when Bradyrhizobium japonicum, the nitrogen-fixing root-nodule symbiont of soybean, was temperature up-shifted from 28 degrees C t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a62b0d4e175a3313f31b0b83799600da
https://doi.org/10.1046/j.1432-1327.1999.00567.x

Concentration of, and SDS Removal from Proteins Isolated from Multiple two-Dimensional Electrophoresis Gels

Autor: Paola Dainese Hatt, Werner Staudenmann, Manfredo Quadroni, Peter James

Publikováno v: European Journal of Biochemistry. 246:336-343

We have developed a gel electrophoresis system that can concentrate proteins from spots cut out of up to 50 two-dimensional electrophoresis gels. During protein concentration, SDS is substituted with a non-ionic detergent (octyl beta-glucopyranoside)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::668dde8389a78e710dd4526787d3abe7
https://doi.org/10.1111/j.1432-1033.1997.00336.x

Analysis of Global Responses by Protein and Peptide Fingerprinting of Proteins Isolated by Two-Dimensional Gel Electrophoresis. Application to the Sulfate-Starvation Response of Escherichia Coli

Autor: Peter James, Michael A. Kertesz, Manfredo Quadroni, Werner Staudenmann

Publikováno v: European Journal of Biochemistry. 239:773-781

A set of 8 proteins (SSI, sulfate-starvation-induced proteins) was observed by comparative two-dimensional electrophoresis to be induced when Escherichia coli were grown using compounds other than sulfate or cysteine as the sole sulfur source. These

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::926f47afbfa875f444ff3ac1be2c1848
https://doi.org/10.1111/j.1432-1033.1996.0773u.x

A Novel EF-Hand Ca2+-Binding Protein from Abdominal Muscle of Crustaceans with Similarity to Calcyphosine from Dog Thyroidea

Autor: Arnold Sauter, Claus W. Heizmann, Werner Staudenmann, Graham J. Hughes

Publikováno v: European Journal of Biochemistry. 227:97-101

The amino acid sequence of a novel EF-hand Ca(2+)-binding protein from the abdominal muscle of the crayfish, Orconectes limosus, has been elucidated by tandem mass spectrometry and automated Edman degradation. The name CCBP-23 (23-kDa crustacean Ca(2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d6b7d641610b54545a51e066bdcdde9
https://doi.org/10.1111/j.1432-1033.1995.tb20363.x

Ser2 is the autophosphorylation site in the beta subunit from bicistronically expressed human casein kinase-2 and from native rat liver casein kinase-2beta

Autor: Peter James, Olaf-Georg Issinger, Werner Staudenmann, Brigitte Boldyreff

Publikováno v: Boldyreff, B, James, P, Staudenmann, W & Issinger, O G 1993, ' Ser2 is the autophosphorylation site in the beta subunit from bicistronically expressed human casein kinase-2 and from native rat liver casein kinase-2 beta ', European Journal of Biochemistry, vol. 218, no. 2, pp. 515-521 .

Udgivelsesdato: 1993-Dec-1 Human casein kinase-2 (CK-2) subunits alpha and beta were bicistronically expressed in bacteria. The recombinant holoenzyme shared all investigated properties with the native CK-2 from mammalian sources (rat liver, Krebs II

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6831e9df29f1355a0dc096c46be0cde
https://doi.org/10.1111/j.1432-1033.1993.tb18404.x