Understanding the Structural Requirements for Activators of the Kef Bacterial Potassium Efflux System

Autor: Tim Rasmussen, Silvia Ekkerman, Garrett M. Morris, Samantha Miller, Samuel C. Grayer, Morgiane Richard, Wendy Bartlett, Jessica Healy, Stuart J. Conway, Christos Pliotas, Ian R. Booth

Publikováno v: Healy, J, Ekkerman, S, Pliotas, C, Richard, M, Bartlett, W, Grayer, S C, Morris, G M, Miller, S, Booth, I R, Conway, S J & Rasmussen, T 2014, ' Understanding the structural requirements for activators of the Kef bacterial potassium efflux system ', Biochemistry, vol. 53, no. 12, pp. 1982–1992 . https://doi.org/10.1021/bi5001118
Biochemistry

This work was supported by The Wellcome Trust (WT092552MA), The Leverhulme Trust (EM-2012-60\2), BBSRC SysMO (BB/F003455/1), a European Union Marie Curie ITN Award (NICHE; 289384) The potassium efflux system, Kef, protects bacteria against the detrim

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https://doi.org/10.1021/bi5001118

Sensing bilayer tension: bacterial mechanosensitive channels and their gating mechanisms

Autor: Wendy Bartlett, Tim Rasmussen, Michelle D. Edwards, Samantha Miller, Susan Shirley Black, Akiko Rasmussen, Ian R. Booth

Publikováno v: Biochemical Society Transactions. 39:733-740

Mechanosensitive channels sense and respond to changes in bilayer tension. In many respects, this is a unique property: the changes in membrane tension gate the channel, leading to the transient formation of open non-selective pores. Pore diameter is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0ecbccd7cfea1cc61e64da910c885cb
https://doi.org/10.1042/bst0390733

The critical role of S-lactoylglutathione formation during methylglyoxal detoxification in Escherichia coli

Autor: Claire A. Walker, Samantha Miller, Wendy Bartlett, Ertan Ozyamak, Morag MacLean, Ian R. Booth, Susan Shirley Black

Publikováno v: Molecular Microbiology

Survival of exposure to methylglyoxal (MG) in Gram-negative pathogens is largely dependent upon the operation of the glutathione-dependent glyoxalase system, consisting of two enzymes, GlxI (gloA) and GlxII (gloB). In addition, the activation of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8754a266ea24e75b06e727f8f4406794
http://europepmc.org/articles/PMC3412212

Pore Mutations of the Escherichia coli MscS Channel Affect Desensitization but Not Ionic Preference

Autor: Wendy Bartlett, Michelle D. Edwards, Ian R. Booth

Publikováno v: Biophysical Journal

Mechanosensitive channels rescue bacterial cells from a fate of lysis when they transfer from a high- to low-osmolarity environment. Of three Escherichia coli mechanosensitive proteins studied to date, only MscS-Ec demonstrates a small anionic prefer

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http://europepmc.org/articles/PMC2275705

A role for mechanosensitive channels in survival of stationary phase: Regulation of channel expression by RpoS

Autor: Richard L. Gourse, Wendy Bartlett, Neil R. Stokes, Samantha Miller, Petra Louis, Ian R. Booth, Chandrasekaran Subramaniam, Heath Murray

Publikováno v: Proceedings of the National Academy of Sciences. 100:15959-15964

The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase. Reduced levels of MS p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef3d1bdcb2f113e389e7fc8fd985f2bf
https://doi.org/10.1073/pnas.2536607100