Výsledky vyhledávání

Akademický článek

A High-Throughput Screen Identifies 2,9-Diazaspiro[5.5]Undecanes as Inducers of the Endoplasmic Reticulum Stress Response with Cytotoxic Activity in 3D Glioma Cell Models.

Publikováno v: PLoS ONE, Vol 11, Iss 8, p e0161486 (2016)

The endoplasmic reticulum (ER) is involved in Ca2+ signaling and protein folding. ER Ca2+ depletion and accumulation of unfolded proteins activate the molecular chaperone GRP78 (glucose-regulated protein 78) which in turn triggers the ER stress respo

Externí odkaz: https://doaj.org/article/32648e68d1c64d85b6cf13c0dd1ec1af

Zobrazit plný text záznamu

Akademický článek

Differential scanning fluorometry signatures as indicators of enzyme inhibitor mode of action: case study of glutathione S-transferase.

Autor: Wendy A Lea, Anton Simeonov

Publikováno v: PLoS ONE, Vol 7, Iss 4, p e36219 (2012)

Differential scanning fluorometry (DSF), also referred to as fluorescence thermal shift, is emerging as a convenient method to evaluate the stabilizing effect of small molecules on proteins of interest. However, its use in the mechanism of action stu

Externí odkaz: https://doaj.org/article/9b20e88ae0844befb57ed2ccc256c249

Zobrazit plný text záznamu

Akademický článek

A high-throughput approach for identification of novel general anesthetics.

Autor: Wendy A Lea, Jin Xi, Ajit Jadhav, Louis Lu, Christopher P Austin, Anton Simeonov, Roderic G Eckenhoff

Publikováno v: PLoS ONE, Vol 4, Iss 9, p e7150 (2009)

Anesthetic development has been a largely empirical process. Recently, we described a GABAergic mimetic model system for anesthetic binding, based on apoferritin and an environment-sensitive fluorescent probe. Here, a competition assay based on 1-ami

Externí odkaz: https://doaj.org/article/adfdbe65fa4e41e2a774442c8ec64d72

Zobrazit plný text záznamu

Akademický článek

Polycystin-1 Interacting Protein-1 (CU062) Interacts with the Ectodomain of Polycystin-1 (PC1)

Autor: Wendy A. Lea, Thomas Winklhofer, Lesya Zelenchuk, Madhulika Sharma, Jessica Rossol-Allison, Timothy A. Fields, Gail Reif, James P. Calvet, Jason L. Bakeberg, Darren P. Wallace, Christopher J. Ward

Publikováno v: Cells, Vol 12, Iss 17, p 2166 (2023)

The PKD1 gene, encoding protein polycystin-1 (PC1), is responsible for 85% of cases of autosomal dominant polycystic kidney disease (ADPKD). PC1 has been shown to be present in urinary exosome−like vesicles (PKD−ELVs) and lowered in individuals w

Externí odkaz: https://doaj.org/article/cb0efb4fcbe4484790ed4e7ad2759ca2

Zobrazit plný text záznamu

Plný text ve formátu HTML

Data from Biochemical Assays for the Discovery of TDP1 Inhibitors

Autor: Yves Pommier, Anton Simeonov, William L. Jorgensen, Ajit Jadhav, David J. Maloney, Rui Gao, Junko Murai, Ganesha Rai, Andrew S. Rosenthal, Andrew G. Stephen, Alena Naumova, Adel Chergui, Bryan T. Mott, Wendy A. Lea, Thomas S. Dexheimer, Shar-yin N. Huang, Christophe Marchand

Drug screening against novel targets is warranted to generate biochemical probes and new therapeutic drug leads. TDP1 and TDP2 are two DNA repair enzymes that have yet to be successfully targeted. TDP1 repairs topoisomerase I–, alkylation-, and cha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1da67e855c35dcbf7a605e7960b2fa0d
https://doi.org/10.1158/1535-7163.c.6536313

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání