A cost-effective approach to DNA methylation detection by Methyl Sensitive DArT sequencing.

Autor: Wendell Jacinto Pereira, Marília de Castro Rodrigues Pappas, Dario Grattapaglia, Georgios Joannis Pappas

Publikováno v: PLoS ONE, Vol 15, Iss 6, p e0233800 (2020)

Several studies suggest the relation of DNA methylation to diseases in humans and important phenotypes in plants drawing attention to this epigenetic mark as an important source of variability. In the last decades, several methodologies were develope

Externí odkaz: https://doaj.org/article/03cd71454631446693f8df8d5ba5a32b

[PROVISIONAL] Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

Autor: Wendell Jacinto Pereira, Arthur Tavares de Oliveira Melo, Alexandre Siqueira Guedes Coelho, Fabiana Aparecida Rodrigues, Sujan Mamidi, Sérgio Amorim de Alencar, Anna Cristina Lanna, Paula Arielle Mendes Ribeiro Valdisser, Claudio Brondani, Ivanildo Ramalho do Nascimento-Júnior, Tereza Cristina de Oliveira Borba, Rosana Pereira Vianello

Publikováno v: Genetics and Molecular Biology

Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of

Externí odkaz: https://doaj.org/article/5df1ac8dc54447ef9522c6f62213788b

An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR

Autor: Priscila Z. Bassinello, Wendell J. Pereira, Claudio Brondani, Rosana Pereira Vianello

Publikováno v: Crop Breeding and Applied Biotechnology, Volume: 17, Issue: 2, Pages: 150-158, Published: JUN 2017
Crop Breeding and Applied Biotechnology, Vol 17, Iss 2, Pp 150-158 (2017)
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Crop Breeding and Applied Biotechnology v.17 n.2 2017
Crop Breeding and Applied Biotechnology
Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB

An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory rou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6d494bd1a57f718e1330aa07aee68f1
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200150&lng=en&tlng=en