Zobrazeno 1 - 10
of 121
pro vyhledávání: '"Wen-Hwei Hsu"'
Publikováno v:
PLoS ONE, Vol 7, Iss 10, p e48301 (2012)
Lysine racemase, a pyridoxal 5'-phosphate (PLP)-dependent amino acid racemase that catalyzes the interconversion of lysine enantiomers, is valuable to serve as a novel non-antibiotic selectable marker in the generation of transgenic plants. Here, we
Externí odkaz:
https://doaj.org/article/408e42572c704cbf84a37e105486c856
Autor:
Yu Tsou, Wen-Hwei Hsu, Ming-Te Yang, Tung-Yueh Lin, Wen-Ching Wang, Jong-Yih Lin, Jai-Shin Liu, Yi-Chia Kuan
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
Scientific Reports
Bioconversion is useful to produce optically pure enantiomers in the pharmaceutical industry, thereby avoiding problems with side reactions during organic synthesis processes. A short-chain dehydrogenase/reductase from Serratia marcescens BCRC 10948
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fa3a4975d0e909685480aef7070dc05
http://link.springer.com/article/10.1038/s41598-018-19235-y
http://link.springer.com/article/10.1038/s41598-018-19235-y
Autor:
Guan-Jhih Peng, Ming-Te Yang, Wen-Hwei Hsu, Yi-Chia Kuan, Jia-Shin Lin, Hsiao-Yi Chou, Tze-Kai Fu
Publikováno v:
Journal of Biotechnology. 170:6-9
(R)-Phenylephrine [(R)-PE] is an α1-adrenergic receptor agonist and is widely used as a nasal decongestant to treat the common cold without the side effects of other ephedrine adrenergic drugs. We identified a short-chain dehydrogenase/reductase (SM
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4f86c9653b065b91be3235fa0a3c4e2
https://doi.org/10.1016/j.jbiotec.2013.11.011
https://doi.org/10.1016/j.jbiotec.2013.11.011
Publikováno v:
Process Biochemistry. 48:1509-1515
Background An amino alcohol dehydrogenase gene (RE_AADH) from Rhodococcus erythropolis BCRC 10909 has been used for the conversion of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone (HPMAE) to (S)-phenylephrine [(S)-PE]. However RE_AADH uses NADPH as co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7edf3940250f87f6fe815506349799a0
https://doi.org/10.1016/j.procbio.2013.07.006
https://doi.org/10.1016/j.procbio.2013.07.006
Publikováno v:
Process Biochemistry. 47:948-952
ATP is required as a structural activator for the reversible epimerization of N-acetyl- d -glucosamine to N-acetyl- d -mannosamine by N-acetyl- d -glucosamine 2-epimerase (AGE); however, the ATP-binding site on AGE has not been clearly identified. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71490c2065c42aa8395254210d23d37e
https://doi.org/10.1016/j.procbio.2012.03.005
https://doi.org/10.1016/j.procbio.2012.03.005
Publikováno v:
Process Biochemistry. 46:1914-1920
A lysine racemase gene (lyr) that consisted of an open reading frame of 1224-bp and encoded a protein with a calculated molecular mass of 45 kDa was cloned from the Proteus mirabilis BCRC10725 and expressed in Escherichia coli BL21(DE3). The purified
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f5f18cd4eabe9d62b1442fdf35d040d1
https://doi.org/10.1016/j.procbio.2011.06.019
https://doi.org/10.1016/j.procbio.2011.06.019
Publikováno v:
Current Microbiology. 62:1282-1286
A CP1201 RIR1 intein is found in the ribonucleotide reductase alpha subunit (RNR α subunit) protein of lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078. This intein can be over-expressed and spliced in Escherichia coli NovaBlue c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::939354e48aef754e5cc0e4c6234f2155
https://doi.org/10.1007/s00284-010-9854-4
https://doi.org/10.1007/s00284-010-9854-4
Publikováno v:
Process Biochemistry. 45:1529-1536
(R)-phenylephrine [(R)-PE] is an α1-adrenergic receptor agonist that is widely used in over-the-counter drugs to treat the common cold. We found that Rhodococcus erythropolis BCRC 10909 can convert detectable level of 1-(3-hydroxyphenyl)-2-(methylam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c7ce14fcbd8c741a6cebf337e8e84ae8
https://doi.org/10.1016/j.procbio.2010.06.003
https://doi.org/10.1016/j.procbio.2010.06.003
Publikováno v:
Process Biochemistry. 45:667-674
A bi-enzyme process for the synthesis of l -homophenylalanine ( l -HPA) from N-carbamoyl- d -homophenylalanine with immobilized N-acylamino acid racemase (racemase) and immobilized N-carbamoyl- l -amino acid amidohydrolase ( l -N-carbamoylase) was de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9f991cc4317eb547d71356526ac66b98
https://doi.org/10.1016/j.procbio.2009.12.017
https://doi.org/10.1016/j.procbio.2009.12.017
Autor:
Long-Liu Lin, Hsin-Mao Wu, Hui-Yu Hu, Wen-Hwei Hsu, Chao-Hung Kao, Wen-Ching Wang, Chao-Kai Lo
Publikováno v:
Process Biochemistry. 44:309-315
Brevibacillus agri dihydropyrimidinase (BaDHP) exhibits a substrate preference for d -homophenylalanylhydantoin ( d -HPAH). Site-directed mutagenesis of BaDHP was performed specifically to the residues proposed to be important in the enzyme activity.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a510bea8d86b996cd5d7c28d2f8750a5
https://doi.org/10.1016/j.procbio.2008.11.005
https://doi.org/10.1016/j.procbio.2008.11.005