Zobrazeno 1 - 10
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pro vyhledávání: '"Wen Jing Y. Tang"'
Transcripts approximately 14.5 kilobases in length from 14 different genes that encode for dynein heavy chains have been identified in poly(A)+ RNA from sea urchin embryos. Analysis of the changes in level of these dynein transcripts in response to d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a249b13957c45fd4fe0c8546ec2e99df
https://europepmc.org/articles/PMC301009/
https://europepmc.org/articles/PMC301009/
Autor:
Wen-Jing Y. Tang
Publisher Summary This chapter highlights blot-affinity purification (BAP) of antibodies. BAP utilizes the superb resolving power of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to separate proteins in a mixture and the subseq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::709ed439990fa5bb68b0213a316c57a9
https://doi.org/10.1016/s0091-679x(08)60245-9
https://doi.org/10.1016/s0091-679x(08)60245-9
Publikováno v:
Biology of the cell. 76(3)
By making the hypothetis that the pattern of conserved sequence residues in the vicinity of the hydrolytic ATP-binding site of dynein would resemble that in myosins from a broad variety of sources, we designed degenerate oligonucleotide primers capab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38a70d3afcdf196d8ea8915765af2d0d
https://pubmed.ncbi.nlm.nih.gov/1305477
https://pubmed.ncbi.nlm.nih.gov/1305477
Autor:
Cheryl A. Phillipson, Wen-Jing Y. Tang, Nathan S. Ching, Gabor Mocz, David J. Asai, I. R. Gibbons, Hening Ren, Gregory J. Dolecki, Barbara H. Gibbons
Publikováno v:
Scopus-Elsevier
A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step, the porti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b9ecb83be477aa3c31b6876e89aa187
https://europepmc.org/articles/PMC52549/
https://europepmc.org/articles/PMC52549/
Publikováno v:
The Journal of Cell Biology
Substitution of any of a variety of organic anions, including acetate, propionate, lactate, gluconate, and succinate, for chloride in the reactivation medium improves the motility of demembranated sperm of Tripneustes gratilla. At the optimum concent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c4e669e0ba5d02c52c13c9f08061947
https://doi.org/10.1083/jcb.101.4.1281
https://doi.org/10.1083/jcb.101.4.1281
Autor:
Gabor Mocz, Barbara H. Gibbons, I. R. Gibbons, A. Lee-Eiford, Wen-Jing Y. Tang, C. A. Phillipson
Publikováno v:
Journal of Biological Chemistry. 262:17728-17734
Irradiation of soluble dynein 1 from sea urchin sperm flagella at 365 nm in the presence of MgATP and 0.05-50 microM vanadate (Vi) cleaves the alpha and beta heavy chains (Mr 428,000) at their V1 sites to give peptides of Mr 228,000 and 200,000, with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2ee5cedb1204ae8135e4a7930804f922
https://doi.org/10.1016/s0021-9258(18)45440-3
https://doi.org/10.1016/s0021-9258(18)45440-3
Publikováno v:
Journal of Biological Chemistry. 257:508-515
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e645e2c44b5eb425f65ed0b0277a1883
https://doi.org/10.1016/s0021-9258(19)68393-6
https://doi.org/10.1016/s0021-9258(19)68393-6
Autor:
Karl H. Martinson, Winfield S. Sale, Marie Paule Cosson, John A. Evans, Becky Houck, Wen-Jing Y. Tang, Barbara H. Gibbons, I. R. Gibbons
Publikováno v:
Proceedings of the National Academy of Sciences. 75:2220-2224
The motility of demembranated sea urchin sperm flagella and that of embryo cilia reactivated with 0.1 mM ATP are completely inhibited by 4 micron and 0.5 micron vanadium(V) [V(V), in vanadate], respectively. The Mg2+-activated ATPase activity (ATP ph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06e3067c9ac3e3f01b54e3bb4bf5341e
https://doi.org/10.1073/pnas.75.5.2220
https://doi.org/10.1073/pnas.75.5.2220
Publikováno v:
Journal of Cell Science. 60:231-249
Treatment of demembranated aea-urchin sperm for 1-2 min with 10μMW-cthylmaleimide (Mall-NEt) at pH80 prior to reactivation with 1 mM-ATP causes the asymmetry of the flagellar waveform to become desensitized to the presence or absence of Ca2+ in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4288e97194050ae153e55d23d9db0c06
https://doi.org/10.1242/jcs.60.1.231
https://doi.org/10.1242/jcs.60.1.231
Publisher Summary This chapter describes the preparation, storage, use, and properties of dynein ATPase from sea urchin sperm flagella, which is probably one of the best available sources for providing relatively large quantities of this enzyme. Sele
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e8891f215cebf3544f9680c512557308
https://doi.org/10.1016/s0091-679x(08)60666-4
https://doi.org/10.1016/s0091-679x(08)60666-4