Origin, distribution, and function of three frequent coding polymorphisms in the gene for the human P2X7 ion channel

Autor: Waldemar Schäfer, Tobias Stähler, Carolina Pinto Espinoza, Welbeck Danquah, Jan Hendrik Knop, Björn Rissiek, Friedrich Haag, Friedrich Koch-Nolte

Publikováno v: Frontiers in Pharmacology, Vol 13 (2022)

P2X7, an ion channel gated by extracellular ATP, is widely expressed on the plasma membrane of immune cells and plays important roles in inflammation and apoptosis. Several single nucleotide polymorphisms have been identified in the human P2RX7 gene.

Externí odkaz: https://doaj.org/article/26f84250e7004adb9dcfa2953cc3b8fb

Development of Antibody and Nanobody Tools for P2X7

Autor: Tobias, Stähler, Welbeck, Danquah, Melanie, Demeules, Henri, Gondé, Romain, Hardet, Friedrich, Haag, Sahil, Adriouch, Friedrich, Koch-Nolte, Stephan, Menzel

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2510

Antibodies that recognize the ATP-gated P2X7 ion channel are etablished research tools. Nanobodies correspond to the antigen-binding variable immunoglobulin domain (VHH) of heavy chain antibodies that naturally occur in camelids. Nanobodies display b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1f58609bce65930b8f87ffb5877500b2
https://pubmed.ncbi.nlm.nih.gov/35776322

Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers

Autor: Hagen von Briesen, Robyn L. Stanfield, Andrew B. Ward, Welbeck Danquah, Sarah Kalusche, Jonathan L. Torres, Friedrich Koch-Nolte, Eric R. Geertsma, Ulrich Wernery, Kamal A. Khazanehdari, Ian A. Wilson, Kathrin Koch, Ursula Dietrich

Publikováno v: Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)

Broadly neutralizing antibodies (bnAbs) against HIV-1 protect from infection and reduce viral load upon therapeutic applications. However no vaccine was able so far to induce bnAbs demanding their expensive biotechnological production. For clinical a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e91b0a9d976d1a631b1bb3ab82b7d33c
https://doi.org/10.1038/s41598-017-08273-7

Nanobodies that block gating of the P2X7 ion channel ameliorate inflammation

Autor: Friedrich Koch-Nolte, Eva Tolosa, Friedrich Haag, Wendy Rotthier, Eric Boué-Grabot, Philine Bergmann, Björn Rissiek, Welbeck Danquah, Joana Assuncao, Nicole Schwarz, Jan-Hendrik Knop, Catherine Meyer-Schwesinger, Tim Magnus, Anna Hammel, Domenica Iacenda, Arnau Serracant-Prat, Carolina Pinto, Peter Bannas, Catelijne Stortelers, Toon Laeremans, Miriam Amadi

Publikováno v: Science Translational Medicine
Science Translational Medicine, American Association for the Advancement of Science, 2016, 8 (366), pp.366ra162-366ra162. ⟨10.1126/scitranslmed.aaf8463⟩
Science Translational Medicine, American Association for the Advancement of Science, 2016, 8 (366), pp.366ra162. ⟨10.1126/scitranslmed.aaf8463⟩

International audience; Ion channels are desirable therapeutic targets, yet ion channel-directed drugs with high selectivity and few side effects are still needed. Unlike small-molecule inhibitors, antibodies are highly selective for target antigens

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17b68a5148cba77b32364230b1c93863
https://pubmed.ncbi.nlm.nih.gov/27881823

Technical Advance: a new cell preparation strategy that greatly improves the yield of vital and functional Tregs and NKT cells

Autor: Friedrich Koch-Nolte, Björn Rissiek, Friedrich Haag, Welbeck Danquah

Publikováno v: Journal of leukocyte biology. 95(3)

Release of NAD+ during preparation of murine lymphocytes causes enzymatic ADP-ribosylation of cell-surface proteins on T cells, catalyzed by toxin-related ecto-ADP-ribosyltransferase, ARTC2. ADP-riboslyation activates the cytolytic P2X7 ion channel a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53c372077863c882b34c9d171837d17b
https://pubmed.ncbi.nlm.nih.gov/24212099