Zobrazeno 1 - 10
of 674
pro vyhledávání: '"Weishan W"'
Autor:
Zhiheng Yang, Bixiao Li, Ruihong Bu, Zhengduo Wang, Zhenguo Xin, Zilong Li, Lixin Zhang, Weishan Wang
Publikováno v:
Synthetic and Systems Biotechnology, Vol 9, Iss 4, Pp 658-666 (2024)
Parageobacillus thermoglucosidasius is emerging as a highly promising thermophilic organism for metabolic engineering. The utilization of CRISPR-Cas technologies has facilitated programmable genetic manipulation in P. thermoglucosidasius. However, th
Externí odkaz:
https://doaj.org/article/2c23e1aa4d6243e3a727d0377ea52504
Publikováno v:
Microbial Biotechnology, Vol 17, Iss 5, Pp n/a-n/a (2024)
Abstract The availability of an alternative and efficient genetic editing technology is critical for fundamental research and strain improvement engineering of Streptomyces species, which are prolific producers of complex secondary metabolites with s
Externí odkaz:
https://doaj.org/article/c026aab9107440858efff6496ae96dce
Autor:
Xinyu Shi, Hao Yan, Fang Yuan, Guoying Li, Jingfang Liu, Chunli Li, Xiaobin Yu, Zilong Li, Yunping Zhu, Weishan Wang
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
Transglutaminase (EC 2.3.2.13, TGase), an enzyme that catalyzes the formation of covalent cross-links between protein or peptide molecules, plays a critical role in commercial food processing, medicine, and textiles. TGase from Streptomyces is the so
Externí odkaz:
https://doaj.org/article/eb963b378ccc4b00a4f91d0963be00f7
Autor:
Zhiheng Yang, Zilong Li, Bixiao Li, Ruihong Bu, Gao-Yi Tan, Zhengduo Wang, Hao Yan, Zhenguo Xin, Guojian Zhang, Ming Li, Hua Xiang, Lixin Zhang, Weishan Wang
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract Thermophilic cell factories have remarkably broad potential for industrial applications, but are limited by a lack of genetic manipulation tools and recalcitrance to transformation. Here, we identify a thermophilic type I-B CRISPR-Cas system
Externí odkaz:
https://doaj.org/article/0c99f7fbbac344ee8beec11c14c75b07
Autor:
Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, Gao-Yi Tan
Publikováno v:
STAR Protocols, Vol 4, Iss 3, Pp 102435- (2023)
Summary: Large biosynthetic gene cluster (BGC) cloning is important for discovering natural product-based drugs and remains challenging in high GC content microorganisms (e.g., Actinobacteria). Here, we present an in vitro CRISPR-Cas12a-mediated prot
Externí odkaz:
https://doaj.org/article/1d047528f6144c03aec0b9aabdac53e8
Publikováno v:
BMC Musculoskeletal Disorders, Vol 24, Iss 1, Pp 1-14 (2023)
Abstract Objective As one of the most important protein-degrading enzymes, ADAMTS-5 plays an important role in the regulation of cartilage homeostasis, while miRNA-140 is specifically expressed in cartilage, which can inhibit the expression of ADAMTS
Externí odkaz:
https://doaj.org/article/754347c5c4b44819b3ca183420336621
TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair
Autor:
Qiang Wei, Dachuan Liu, Genglei Chu, Qifan Yu, Zhao Liu, Jiaying Li, Qingchen Meng, Weishan Wang, Fengxuan Han, Bin Li
Publikováno v:
Bioactive Materials, Vol 19, Iss , Pp 581-593 (2023)
Annulus fibrosus (AF) repair remains a challenge because of its limited self-healing ability. Endogenous repair strategies combining scaffolds and growth factors show great promise in AF repair. Although the unique and beneficial characteristics of d
Externí odkaz:
https://doaj.org/article/695d19217e784248993ef77357a55a12
Akademický článek
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Autor:
Xiangxiang Zhao, Zhengduo Wang, Bowen Yang, Zilong Li, Yaojun Tong, Yuhai Bi, Zhenghong Li, Xuekui Xia, Xiangyin Chen, Lixin Zhang, Weishan Wang, Gao-Yi Tan
Publikováno v:
Synthetic and Systems Biotechnology, Vol 6, Iss 4, Pp 283-291 (2021)
Antigen detection provides particularly valuable information for medical diagnoses; however, the current detection methods are less sensitive and accurate than nucleic acid analysis. The combination of CRISPR/Cas12a and aptamers provides a new detect
Externí odkaz:
https://doaj.org/article/877d00583c824ff2bec6850f1e3cdc8f
Autor:
Tong Shi, Lu Zhang, Mindong Liang, Weishan Wang, Kefeng Wang, Yue Jiang, Jing Liu, Xinwei He, Zhiheng Yang, Haihong Chen, Chuan Li, Dongyuan Lv, Liming Zhou, Biqin Chen, Dan Li, Li-Xin Zhang, Gao-Yi Tan
Publikováno v:
Synthetic and Systems Biotechnology, Vol 6, Iss 4, Pp 335-342 (2021)
The versatile photosynthetic α-proteobacterium Rhodobacter sphaeroides, has recently been extensively engineered as a novel microbial cell factory (MCF) to produce pharmaceuticals, nutraceuticals, commodity chemicals and even hydrogen. However, ther
Externí odkaz:
https://doaj.org/article/f17df948cbda4dd286efdfb00a399954