Designed active-site library reveals thousands of functional GFP variants.

Autor: Weinstein JY; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel., Martí-Gómez C; Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA., Lipsh-Sokolik R; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel., Hoch SY; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel., Liebermann D; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 7610001, Israel., Nevo R; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel., Weissman H; Department of Molecular Chemistry and Materials Science, Weizmann Institute of Science, Rehovot, 7610001, Israel., Petrovich-Kopitman E; Life science Core facilities, Weizmann Institute of Science, Rehovot, 7610001, Israel., Margulies D; Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot, 7610001, Israel., Ivankov D; Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia., McCandlish DM; Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA., Fleishman SJ; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel. sarel@weizmann.ac.il.

Publikováno v: Nature communications [Nat Commun] 2023 May 20; Vol. 14 (1), pp. 2890. Date of Electronic Publication: 2023 May 20.

De novo-designed transmembrane domains tune engineered receptor functions.

Autor: Elazar A; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel., Chandler NJ; Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia., Davey AS; Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia., Weinstein JY; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel., Nguyen JV; Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia., Trenker R; Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia., Cross RS; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.; Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia., Jenkins MR; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.; Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; La Trobe Institute of Molecular Science, La Trobe University, Bundoora, Victoria, Australia., Call MJ; Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia., Call ME; Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia., Fleishman SJ; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.

Publikováno v: ELife [Elife] 2022 May 04; Vol. 11. Date of Electronic Publication: 2022 May 04.

Combined Crystal Structure of a Type I Cohesin: MUTATION AND AFFINITY BINDING STUDIES REVEAL STRUCTURAL DETERMINANTS OF COHESIN-DOCKERIN SPECIFICITIES.

Autor: Cameron K; From the CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal., Weinstein JY; the Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel, and., Zhivin O; the Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel, and., Bule P; From the CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal., Fleishman SJ; the Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel, and., Alves VD; From the CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal., Gilbert HJ; the Institute of Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom., Ferreira LM; From the CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal., Fontes CM; From the CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal., Bayer EA; the Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel, and Ed.Bayer@weizmann.ac.il., Najmudin S; From the CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2015 Jun 26; Vol. 290 (26), pp. 16215-25. Date of Electronic Publication: 2015 May 01.