Zobrazeno 1 - 10
of 49
pro vyhledávání: '"Wei-Lih Lee"'
Publikováno v:
Bio-Protocol, Vol 8, Iss 23 (2018)
In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, whic
Externí odkaz:
https://doaj.org/article/1596adac2a8249a09f1827fdb21b302b
Publikováno v:
eLife, Vol 7 (2018)
Cortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to faci
Externí odkaz:
https://doaj.org/article/e924560c68244b31a7a1c6f464c18fd7
Publikováno v:
J Cell Sci
The current model for spindle positioning requires attachment of the microtubule (MT) motor cytoplasmic dynein to the cell cortex, where it generates pulling force on astral MTs to effect spindle displacement. How dynein is anchored by cortical attac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::069dfdff02e65466d2b8a78480e61ce8
https://doi.org/10.1101/2020.03.30.014621
https://doi.org/10.1101/2020.03.30.014621
Publikováno v:
The Journal of Cell Biology
Dynein orients the spindle by pulling on astral microtubules from the cortex. In Saccharomyces cerevisiae, the microtubule-associated protein She1 specifically inhibits dynein in the mother compartment to promote spindle movements toward the bud. Zhu
Publikováno v:
Biophysical Reviews
In both animals and fungi, spindle positioning is dependent upon pulling forces generated by cortically anchored dynein. In animals, cortical anchoring is accomplished by a ternary complex containing the dynein-binding protein NuMA and its cortical a
Publikováno v:
The Journal of Cell Biology
Many aspects of cytoskeletal assembly and dynamics can be recapitulated in vitro; yet, how the cytoskeleton integrates signals in vivo across cellular membranes is far less understood. Recent work has demonstrated that the membrane alone, or through
Publikováno v:
Traffic. 16:773-786
The ability to fluorescently label microtubules in live cells has enabled numerous studies of motile and mitotic processes. Such studies are particularly useful in budding yeast owing to the ease with which they can be genetically manipulated and ima
Publikováno v:
Journal of Cell Science; 2020, Vol. 133 Issue 20, p1-14, 14p
Autor:
Wei-Lih Lee, Yili Zhu
Publikováno v:
Molecular Microbiology. 94:486-489
Aspergillus nidulans is an ideal model to study nuclear migration and intracellular transport by dynein and kinesin owing to its long neuron-like hyphae, conserved transport mechanisms, and powerful genetics. In this organism, as in other filamentous