Zobrazeno 1 - 10
of 461
pro vyhledávání: '"Wei Shou Hu"'
Publikováno v:
PLoS Pathogens, Vol 18, Iss 9, p e1010874 (2022)
Influenza A virus (IAV) infection causes acute respiratory disease with potential severe and deadly complications. Viral pathogenesis is not only due to the direct cytopathic effect of viral infections but also to the exacerbated host inflammatory re
Externí odkaz:
https://doaj.org/article/bba56decc3cd48de889b81735fd7deed
Autor:
Ruben Boon, Manoj Kumar, Tine Tricot, Ilaria Elia, Laura Ordovas, Frank Jacobs, Jennifer One, Jonathan De Smedt, Guy Eelen, Matthew Bird, Philip Roelandt, Ginevra Doglioni, Kim Vriens, Matteo Rossi, Marta Aguirre Vazquez, Thomas Vanwelden, François Chesnais, Adil El Taghdouini, Mustapha Najimi, Etienne Sokal, David Cassiman, Jan Snoeys, Mario Monshouwer, Wei-Shou Hu, Christian Lange, Peter Carmeliet, Sarah-Maria Fendt, Catherine M. Verfaillie
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-16 (2020)
Hepatocytes grown in a dish are immature and do not metabolize compounds as a real liver would. Here, the authors supply stem cell-derived hepatocytes with amino acids at a higher concentration than nutritionally necessary, changing the metabolism of
Externí odkaz:
https://doaj.org/article/0a789642bd074590a245141b427513bf
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Enterococcus faecalis is considered a leading cause of hospital-acquired infections. Treatment of these infections has become a major challenge for clinicians because some E. faecalis strains are resistant to multiple clinically used antibiotics. Mor
Externí odkaz:
https://doaj.org/article/bb1e51a3d260480d8a2910c44812f70b
Publikováno v:
ACS synthetic biology. 11(10)
Recombinant adeno-associated viruses (rAAV) are important gene delivery vehicles for gene therapy applications. Their production relies on plasmid transfection or virus infection of producer cells, which pose a challenge in process scale-up. Here, we
Publikováno v:
Human Gene Therapy. 34:162-170
An important quality attribute of a recombinant Adeno-Associated Virus (rAAV) as a therapeutic vector is its infectivity. Current assays to quantify infectious rAAV rely on coinfection with a helper virus such as adenovirus, which requires helper vir
Publikováno v:
Biotechnology and Bioengineering. 120:216-229
Over the last two decades, model-based metabolic pathway optimization tools have been developed for the design of microorganisms to produce desired metabolites. However, few have considered more complex cellular systems such as mammalian cells, which
Autor:
Juan Antonio García-León, Manoj Kumar, Ruben Boon, David Chau, Jennifer One, Esther Wolfs, Kristel Eggermont, Pieter Berckmans, Nilhan Gunhanlar, Femke de Vrij, Bas Lendemeijer, Benjamin Pavie, Nikky Corthout, Steven A. Kushner, José Carlos Dávila, Ivo Lambrichts, Wei-Shou Hu, Catherine M. Verfaillie
Publikováno v:
Stem Cell Reports, Vol 10, Iss 2, Pp 655-672 (2018)
Summary: Scarce access to primary samples and lack of efficient protocols to generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding of OL biology and the development of novel therapies. Here, we demo
Externí odkaz:
https://doaj.org/article/397f44eba5cf4003894bb0d26e27bd32
Publikováno v:
Viruses, Vol 13, Iss 11, p 2200 (2021)
The Madin–Darby Canine Kidney (MDCK) cell line is among the most commonly used cell lines for the production of influenza virus vaccines. As cell culture-based manufacturing is poised to replace egg-based processes, increasing virus production is o
Externí odkaz:
https://doaj.org/article/b15f7dc0ab99456f88665db0217b1cdf
Publikováno v:
Trends in Biotechnology.
Autor:
Haiyun Pei, Hsu-Yuan Fu, Hiroyuki Hirai, Dong Seong Cho, Timothy D. O'Brien, James Dutton, Catherine M. Verfaillie, Wei-Shou Hu
Publikováno v:
Stem Cell Research, Vol 21, Iss C, Pp 132-136 (2017)
We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripote
Externí odkaz:
https://doaj.org/article/45107a916851422fa126eb3aa6ca581e