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pro vyhledávání: '"Wayne Wray"'
Autor:
Virginia P. Wray, Wayne Wray
Publikováno v:
Chromosomal Nonhistone Proteins ISBN: 9781351070690
Chromosomal Nonhistone Proteins
Chromosomal Nonhistone Proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7186206d5a01951b9d7dc0bbc6692771
https://doi.org/10.1201/9781351070690-4
https://doi.org/10.1201/9781351070690-4
Publikováno v:
Experimental Cell Research. 100:204-212
The topological features of isolated Chinese hamster ovary metaphase chromosomes were studied with high resolution scanning electron microscopy (SEM) using the techniques of direct current sputtering for the deposition of metal on the specimens. Meta
Publikováno v:
Analytical Biochemistry. 118:197-203
The silver-staining procedure for detecting proteins in polyacrylamide gels has been modified and further simplified so that it is stable, controllable, and even more rapid than previous silver-staining methods. The method retains its sensitivity to
Autor:
Wayne Wray, Joannie B. Krajca
Publikováno v:
Histochemistry. 51:103-111
G/Q-banding is a new, rapid, fluorescent technique for banding isolated chromosomes that incorporates characteristics of both G- and Q- banding. G-bands, while easily characterized, are often inconsistent when using isolated chromosomes, and Q-bands,
Autor:
David L. Miller, Arthur J. Blume, Michael P. Vitek, Evelyn A. Devine, Wayne Wray, W. Ted Brown, Debra Mullikin-Kilpatrick, Regina Dutkowski
Publikováno v:
Gene Analysis Techniques. 2:16-21
Chromosome 21 is the only human chromosome in the mouse human hybrid, WA17. WA17 cells were treated with 0.1 μg/ml Colcemid, lysed, and mitotic chromosomes were separated based on their sizes in sucrose velocity gradients. The position of chromosome
Publikováno v:
Nucleic Acids Research. 8:265-278
Chromatin gels, metaphase chromosomes, and intact nuclei were studied by freeze fracturing followed by electron microscopy. The results complement and extend those obtained by classical electron microscopy techniques as they are obtained without fixa
Autor:
Virginia P. Wray, Wayne Wray
Publikováno v:
Journal of Histochemistry & Cytochemistry. 27:454-457
The proteins on metaphase chromosomes theoretically may be distributed ubiquitously throughout the karyotype, may be present uniquely on individual chromosomes or classes of chromosomes, or may exist in any combination of the above. Separation of chr
Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes
Publikováno v:
Chromosoma. 88:333-342
Prematurely condensed chromosomes (PCC) of HeLa cells synchronized in different phases of the cell cycle were analyzed by high-resolution scanning electron microscopy. The purpose of this study was to examine changes in the arrangement of the basic 3
Autor:
Kim J. Mitchell, Wayne Wray
Publikováno v:
Experimental Cell Research. 123:452-455
Colcemid treatment of cell culture beads confluent with cells released a mitotic population of cells which was as efficient as that obtained by conventional shake-off procedures. The bead system provides an easy and efficient means for harvesting lar
Simultaneous observation of quinacrine bands and silver grains on radiolabeled metaphase chromosomes
Publikováno v:
Histochemistry. 59:1-8
A procedure is described for quinacrine banding of radiolabeled metaphase chromosomes for autoradiography. The chromosomes can be labeled either in vivo or by in situ hybridization. The banding procedure involves treating the slides with RNase and fo