Zobrazeno 1 - 10
of 54
pro vyhledávání: '"Wayne R Gombotz"'
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 3, Iss C (2016)
Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in t
Externí odkaz:
https://doaj.org/article/078ee2b5a8e84bdca9a5a10fd47e457c
Autor:
Daniel C Farley, Laura McCloskey, Barbara A Thorne, Semih U Tareen, Christopher J Nicolai, David J Campbell, Richard Bannister, Hannah J Stewart, Laura JE Pearson, Bentley J Moyer, Scott H Robbins, Leah Zielinski, Tae Kim, Pippa A Radcliffe, Kyriacos A Mitrophanous, Wayne R Gombotz, James E Miskin, Brenna Kelley-Clarke
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 2, Iss , Pp - (2015)
It is a current regulatory requirement to demonstrate absence of detectable replication-competent lentivirus (RCL) in lentiviral vector products prior to use in clinical trials. Immune Design previously described an HIV-1-based integration-deficient
Externí odkaz:
https://doaj.org/article/7f7c5943dd9b4748b580d44545f0e674
Autor:
Brenna Kelley-Clarke, Ozan S. Kumru, David B. Volkin, Witold Cieplak, Sangeeta B. Joshi, C. Wayne R. Gombotz, Yu Wang, Tae Kim
Publikováno v:
Journal of Pharmaceutical Sciences. 107:2764-2774
A replication-deficient lentiviral vector encoding the tumor antigen gene NY-ESO-1 was characterized in terms of vector morphology, particle size range, concentration, and zeta potential using a variety of physical methods. Environmentally stressed v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::667d280d42575124494305c7344e25a4
https://doi.org/10.1016/j.xphs.2018.07.010
https://doi.org/10.1016/j.xphs.2018.07.010
Autor:
Wayne R. Gombotz, Allan S. Hoffman
Publikováno v:
Hydrogels in Medicine and Pharmacy ISBN: 9780429285097
This chapter reviews the preparation and surface characterization of hydrogels. It discusses applications of hydrogels containing immobilized biomolecules and cells. Biomolecules and cells can be immobilized on and within many different supports usin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b3e63711b0a64ea780c3988492eeba2
https://doi.org/10.1201/9780429285097-5
https://doi.org/10.1201/9780429285097-5
Autor:
Htet A. Khant, Wah Chiu, Caroline J. Fu, Wayne R. Gombotz, Benjamin W. Dulken, Tae Hee Kim, Suzie H. Pun, Jenelyn Ramos, Christopher W. Mount
Publikováno v:
Molecular Pharmaceutics. 9:135-143
Polymeric micelles formed by the self-assembly of amphiphilic block copolymers can be used to encapsulate hydrophobic drugs for tumor-delivery applications. Filamentous carriers with high aspect ratios offer potential advantages over spherical carrie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc9832252c0ca9ff3d0068c537a0613c
https://doi.org/10.1021/mp200381c
https://doi.org/10.1021/mp200381c
Autor:
Tsai-Yu Lin, Anshika Bajaj, Lisa Y. Ngo, Peter Berglund, Wayne R Gombotz, Jan ter Meulen, Brenna Kelley-Clarke, Michele E Murphy
Publikováno v:
Cancer Research. 78:5919-5919
Background: ZVex is a novel, integration-deficient, dendritic cell-targeting lentiviral vector platform currently being evaluated in clinical trials in sarcoma patients. The vector is capable of delivering multiple full-length genes simultaneously. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ad784e17badf901f83872ed742408b4f
https://doi.org/10.1158/1538-7445.am2018-5919
https://doi.org/10.1158/1538-7445.am2018-5919
Publikováno v:
Pharmaceutical Research. 27:1900-1913
Indocyanine green (ICG), an FDA-approved near infrared (NIR) dye, has potential application as a contrast agent for tumor detection. Because ICG binds strongly to plasma proteins and exhibits aqueous, photo, and thermal instability, its current appli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f9101319e4d673875bfef1f13172f23
https://doi.org/10.1007/s11095-010-0190-y
https://doi.org/10.1007/s11095-010-0190-y
Autor:
Allan S. Hoffman, Lotte Kreilgaard, Ninus Caram-Lelham, Dean K. Pettit, Wayne R. Gombotz, Chad D. Brown, Masashi Nakakura
Publikováno v:
Journal of Controlled Release. 72:35-46
We have prepared a new formulation for mucosal delivery of GM-CSF or PEGylated GM-CSF based on a chitosan carrier plus added glycerol to control the rate of release of the protein. Thin dry films comprised of various weight ratios of chitosan to glyc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f93264d98a3bbe47162a3ec4dadf2b1d
https://doi.org/10.1016/s0168-3659(01)00260-7
https://doi.org/10.1016/s0168-3659(01)00260-7
Autor:
Dace A. Krasts, Mark Daniels, Alain Balland, Wayne R Gombotz, Julia A Mahan-Boyce, Wesley Wang
Publikováno v:
Journal of Chromatography A. 846:143-156
We present here the purification and the characterization of the isoforms of PIXY321, a genetically engineered fusion of granulocyte-macrophage-colony stimulating factor and interleukin-3 expressed in yeast. The isoforms of PIXY321 were isolated usin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eda980468c8ded52d44a9161f73bb694
https://doi.org/10.1016/s0021-9673(99)00127-2
https://doi.org/10.1016/s0021-9673(99)00127-2
Publikováno v:
Biochemistry. 38:5241-5247
This study elucidates the importance of thermal reversibility as it pertains to the minimization of recombinant human Flt3 ligand aggregation and its potential role for determining solution conditions that can achieve the greatest long-term storage s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90609b34f0637a53caae873444acf4e1
https://doi.org/10.1021/bi982881g
https://doi.org/10.1021/bi982881g