Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Wayne L Ryan"'
Publikováno v:
PLoS ONE, Vol 13, Iss 12, p e0208508 (2018)
This study was undertaken to evaluate a novel method for stabilizing and preserving the original proportion of cell-free fetal DNA (cffDNA) in maternal blood for extended periods of time without using crosslinking agents, such as formaldehyde, which
Externí odkaz:
https://doaj.org/article/0e884f8b0ac74be5be445bd065d5a3c8
Publikováno v:
PLoS ONE, Vol 12, Iss 8, p e0183915 (2017)
Cell-free DNA (cfDNA) in blood is used as a source of genetic material for noninvasive prenatal and cancer diagnostic assays in clinical practice. Recently we have started a project for new biomarker discovery with a view to developing new noninvasiv
Externí odkaz:
https://doaj.org/article/6c38870b9cb94396a4e5aeabdfce731a
Publikováno v:
Clinica chimica acta; international journal of clinical chemistry. 483
Background Plasma cell-free DNA (cfDNA) fragment size distribution provides important information required for diagnostic assay development. We have developed and optimized droplet digital PCR (ddPCR) assays that quantify short and long DNA fragments
Autor:
C. Bassett, D. Bourne, S. Norton, M. R. Fernando, Wayne L. Ryan, Bradford A. Hunsley, G. Krzyzanowski, K. Chen
Publikováno v:
Prenatal Diagnosis. 30:418-424
Objective To develop a standardized blood collection device that preserves fetal cell-free DNA and minimizes the cell-free DNA background in maternal plasma. Methods Blood samples were drawn from healthy pregnant donors into K3EDTA (BD vacutainer®)
Publikováno v:
Journal of Diabetes Science and Technology. 6:483-485
Research shows lifestyle changes and regular monitoring of diabetes indices are the most effective means to manage diabetes.1,2 Measuring hemoglobin A1c (A1C) is an accepted means of diagnosis and a helpful indicator when monitoring diabetes status.
Autor:
Wayne L. Ryan, Alireza Ebrahim
Publikováno v:
Cytometry. 25:156-163
The osmotic lysis procedure was employed to en-capsulate ribonucleic acid (RNA) in human red blood cells in order to prepare a reticulocyte reference control. The procedure required the hypotonic dialysis of erythrocytes in the presence of RNA and cy
Autor:
Bradford A. Hunsley, Wayne L. Ryan
Publikováno v:
Journal of diabetes science and technology. 1(2)
Introduction: There is no available glucose standard for whole blood. None of the various glucose controls can be used as standards (calibrators) for glucose meters or test strips. This article describes the need for a whole blood glucose standard fo
Autor:
Kay Hakenkamp, Wayne L. Ryan
Publikováno v:
Laboratory Medicine. 22:197-202
Aspirin has been employed at several dosage levels for vascular diseases such as angina, myocardial infarction, and hypertension in pregnancy. The beneficial effect of aspirin in these diseases is believed to be from the inhibitory effect of acetylsa
Autor:
Dominic E. Warrino, Samuel J. Pirruccello, Susan Swindells, Mary Hanson, Louis J. DeGennaro, Wayne L. Ryan
Publikováno v:
Journal of immunological methods. 305(2)
We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex BCT were stored at room temper
Publikováno v:
Thrombosis research. 58(2)
A method for screening EDTA (K 3 ) blood samples for platelet function is described. The general availability of whole blood platelet counters in the clinical laboratory suggested their use to measure platelet aggregation. To simplify the collection