Výsledky vyhledávání - "Wayne L Hubbell"

Akademický článek

The structure and dynamics of secretory component and its interactions with polymeric immunoglobulins

Autor: Beth M Stadtmueller, Kathryn E Huey-Tubman, Carlos J López, Zhongyu Yang, Wayne L Hubbell, Pamela J Bjorkman

Publikováno v: eLife, Vol 5 (2016)

As a first-line vertebrate immune defense, the polymeric immunoglobulin receptor (pIgR) transports polymeric IgA and IgM across epithelia to mucosal secretions, where the cleaved ectodomain (secretory component; SC) becomes a component of secretory a

Externí odkaz: https://doaj.org/article/52f1e51b6b204027a57b2fd45eafd024

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Akademický článek

Membrane potential accelerates sugar uptake by stabilizing the outward facing conformation of the Na/glucose symporter vSGLT

Autor: Farha Khan, Matthias Elgeti, Samuel Grandfield, Aviv Paz, Fiona B. Naughton, Frank V. Marcoline, Thorsten Althoff, Natalia Ermolova, Ernest M. Wright, Wayne L. Hubbell, Michael Grabe, Jeff Abramson

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)

Abstract Sodium-dependent glucose transporters (SGLTs) couple a downhill Na+ ion gradient to actively transport sugars. Here, we investigate the impact of the membrane potential on vSGLT structure and function using sugar uptake assays, double electr

Externí odkaz: https://doaj.org/article/14990935ce0547d8bbd11e73b798bc03

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Akademický článek

Neutralizing antibodies induced in immunized macaques recognize the CD4-binding site on an occluded-open HIV-1 envelope trimer

Autor: Zhi Yang, Kim-Marie A. Dam, Michael D. Bridges, Magnus A. G. Hoffmann, Andrew T. DeLaitsch, Harry B. Gristick, Amelia Escolano, Rajeev Gautam, Malcolm A. Martin, Michel C. Nussenzweig, Wayne L. Hubbell, Pamela J. Bjorkman

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)

Neutralizing antibodies (bNAbs) against HIV-1 are exclusively directed against the viral envelope protein (Env) and mainly target Env in a closed, prefusion state. Here, Yang et al. structurally characterize two heterologously-neutralizing CD4-bindin

Externí odkaz: https://doaj.org/article/a3f529a3512548c399bdcaadfab62202

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Conformational dynamics of the μ-opioid receptor determine ligand intrinsic efficacy

Autor: Jiawei Zhao, Matthias Elgeti, Evan S. O’Brien, Cecília P. Sár, Amal EI Daibani, Jie Heng, Xiaoou Sun, Tao Che, Wayne L. Hubbell, Brian K. Kobilka, Chunlai Chen

The μ-opioid receptor (μOR) is an important target for pain management and the molecular understanding of drug action will facilitate the development of better therapeutics. Here we show, using double electron-electron resonance (DEER) and single-m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f00435d56f60300b4684a15212673970
https://doi.org/10.1101/2023.04.28.538657

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Akademický článek

DEER Analysis of GPCR Conformational Heterogeneity

Autor: Matthias Elgeti, Wayne L. Hubbell

Publikováno v: Biomolecules, Vol 11, Iss 6, p 778 (2021)

G protein-coupled receptors (GPCRs) represent a large class of transmembrane helical proteins which are involved in numerous physiological signaling pathways and therefore represent crucial pharmacological targets. GPCR function and the action of the

Externí odkaz: https://doaj.org/article/991163fe50b14ef7b1f532410bbca46e

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Synthetic nanobodies as angiotensin receptor blockers

Autor: Wayne L. Hubbell, Andrew C. Kruse, Robert J. Lefkowitz, Conor McMahon, Howard A. Rockman, Matthias Elgeti, Laura M. Wingler, Jialu Wang, Meredith A. Skiba, Dean P. Staus

Publikováno v: Proc Natl Acad Sci U S A

There is considerable interest in developing antibodies as functional modulators of G protein-coupled receptor (GPCR) signaling for both therapeutic and research applications. However, there are few antibody ligands targeting GPCRs outside of the che

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39b35f906b7b9631e5554ad558505ea4
https://doi.org/10.1073/pnas.2009029117

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Lipid bilayer induces contraction of the denatured state ensemble of a helical-bundle membrane protein

Autor: Kristen A. Gaffney, Ruiqiong Guo, Michael D. Bridges, Shaima Muhammednazaar, Daoyang Chen, Miyeon Kim, Zhongyu Yang, Anthony L. Schilmiller, Nabil F. Faruk, Xiangda Peng, A. Daniel Jones, Kelly H. Kim, Liangliang Sun, Wayne L. Hubbell, Tobin R. Sosnick, Heedeok Hong

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance The conformational properties of denatured states of proteins affect their folding, turnover, translocation, and chaperone action. We find that the denatured state ensemble of a helical membrane protein is richer than depicted in the cla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50205db709e64109988250c0aefcd35d
https://doi.org/10.1073/pnas.2109169119

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Neutralizing antibodies induced in immunized macaques recognize the CD4-binding site on an occluded-open HIV-1 envelope trimer

Autor: Zhi, Yang, Kim-Marie A, Dam, Michael D, Bridges, Magnus A G, Hoffmann, Andrew T, DeLaitsch, Harry B, Gristick, Amelia, Escolano, Rajeev, Gautam, Malcolm A, Martin, Michel C, Nussenzweig, Wayne L, Hubbell, Pamela J, Bjorkman

Publikováno v: Nature communications. 13(1)

Broadly-neutralizing antibodies (bNAbs) against HIV-1 Env can protect from infection. We characterize Ab1303 and Ab1573, heterologously-neutralizing CD4-binding site (CD4bs) antibodies, isolated from sequentially-immunized macaques. Ab1303/Ab1573 bin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::83a470073d9f0024e6db1b68a65f9eec
https://pubmed.ncbi.nlm.nih.gov/35136084

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Viewing rare conformations of the β(2) adrenergic receptor with pressure-resolved DEER spectroscopy

Autor: Rachel A. Matt, Daniel Hilger, Michael T. Lerch, Wayne L. Hubbell, Brian K. Kobilka, Matthieu Masureel, Matthias Elgeti, Kaavya Krishna Kumar, Bryon Foys

Publikováno v: Proc Natl Acad Sci U S A
Proceedings of the National Academy of Sciences of the United States of America, vol 117, iss 50

The β(2) adrenergic receptor (β(2)AR) is an archetypal G protein coupled receptor (GPCR). One structural signature of GPCR activation is a large-scale movement (ca. 6 to 14 Å) of transmembrane helix 6 (TM6) to a conformation which binds and activa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64a637c454c11c427136437ad06c8bd4
https://europepmc.org/articles/PMC7749303/

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Resolving dynamics and function of transient states in single enzyme molecules

Autor: Suren Felekyan, Dmitro Rodnin, Thomas-Otavio Peulen, Hugo Sanabria, Ralf Kühnemuth, Claus A. M. Seidel, Mark R. Fleissner, Felix Koberling, Wayne L. Hubbell, Holger Gohlke, Katherina Hemmen, Mykola Dimura

Publikováno v: Nature Communications 11(1), 1231 (2020). doi:10.1038/s41467-020-14886-w
Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
Nature Communications

We use a hybrid fluorescence spectroscopic toolkit to monitor T4 Lysozyme (T4L) in action by unraveling the kinetic and dynamic interplay of the conformational states. In particular, by combining single-molecule and ensemble multiparameter fluorescen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b59e31c05d5189c882dc3c47e38829d
https://juser.fz-juelich.de/record/874540

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