Method for Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas9

Autor: Jessica M, Walter, Max G, Schubert, Stephanie H, Kung, Kristy, Hawkins, Darren M, Platt, Aaron D, Hernday, Tina, Mahatdejkul-Meadows, Wayne, Szeto, Sunil S, Chandran, Jack D, Newman, Andrew A, Horwitz

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2049

CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3e1543e3182e9bff9ba1b6d32f630f29
https://pubmed.ncbi.nlm.nih.gov/31602604

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

Autor: Aaron D. Hernday, Sunil S. Chandran, Stephanie H. Kung, Wayne Szeto, Jessica M. Walter, Max G. Schubert, Tina Mahatdejkul-Meadows, Kristy Michelle Hawkins, Darren Platt, Jack D. Newman, Andrew A. Horwitz

Publikováno v: Horwitz, AA; Walter, JM; Schubert, MG; Kung, SH; Hawkins, K; Platt, DM; et al.(2015). Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas. Cell Systems, 1(1), 88-96. doi: 10.1016/j.cels.2015.02.001. UC Merced: Retrieved from: http://www.escholarship.org/uc/item/01d6s8m1
Cell systems, vol 1, iss 1

© 2015 The Authors. Summary CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations. Here we show that co-transformation of a single linearized plasmid with m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d27ed41b04db0f82fc173913678fa9e8
https://doi.org/10.1016/j.cels.2015.02.001

Synergistic induction of tumor antigens by Wnt-1 signaling and retinoic acid revealed by gene expression profiling

Autor: Ralph H. Schwall, Debra L. Dugger, David Wieand, P. Mickey Williams, Jane Winer, David Tice, Sharon Fong, Irina Soloviev, Bonnee Rubinfeld, Victoria Smith, Paul Polakis, Wayne Szeto, Diane Pennica

Publikováno v: The Journal of biological chemistry. 277(16)

Novel drug targets can be identified by differential analysis of RNA transcripts isolated from cancer cell lines and tissues. We have extended this approach by analyzing differences in gene expression resulting from the drug treatment of transformed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7be059f2ae8493d32072ebab493251c0
https://pubmed.ncbi.nlm.nih.gov/11832495

Photo and Hormonal Control of Meristem Identity in the Arabidopsis Flower Mutants apetala2 and apetala1

Autor: Jack K. Okamuro, Wayne Szeto, Cynthia Lotys-Prass, K. Diane Jofuku

Publikováno v: The Plant Cell. 9:37

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fa7bdb7baa6a62da0b2723bdb767fd5e
https://doi.org/10.2307/3870369