Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience.

Autor: Cummings CB; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Catania SS; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Ednacot EMQ; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Kinsella-Johnson AJ; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Meeds CE; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Reynolds JW; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Sanderson AE; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Johnson RA; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Watts KR; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University; krwatts@calpoly.edu.

Publikováno v: Journal of visualized experiments : JoVE [J Vis Exp] 2024 Nov 15 (213). Date of Electronic Publication: 2024 Nov 15.

Activation of Energy Metabolism through Growth Media Reformulation Enables a 24-Hour Workflow for Cell-Free Expression.

Autor: Levine MZ; Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, California 93407, United States.; Center for Application in Biotechnology, California Polytechnic State University, San Luis Obispo, California 93407, United States., So B; Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, California 93407, United States.; Center for Application in Biotechnology, California Polytechnic State University, San Luis Obispo, California 93407, United States., Mullin AC; Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, California 93407, United States.; Center for Application in Biotechnology, California Polytechnic State University, San Luis Obispo, California 93407, United States., Fanter R; College of Agriculture, Food and Environmental Sciences, California Polytechnic State University, San Luis Obispo, California 93407, United States., Dillard K; Department of Food Science and Nutrition, California Polytechnic State University, San Luis Obispo, California 93407, United States., Watts KR; Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, California 93407, United States.; Center for Application in Biotechnology, California Polytechnic State University, San Luis Obispo, California 93407, United States., La Frano MR; Department of Food Science and Nutrition, California Polytechnic State University, San Luis Obispo, California 93407, United States.; Center for Health Research, California Polytechnic State University, San Luis Obispo, California 93407, United States., Oza JP; Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, California 93407, United States.; Center for Application in Biotechnology, California Polytechnic State University, San Luis Obispo, California 93407, United States.

Publikováno v: ACS synthetic biology [ACS Synth Biol] 2020 Oct 16; Vol. 9 (10), pp. 2765-2774. Date of Electronic Publication: 2020 Sep 14.

Unlocking Applications of Cell-Free Biotechnology through Enhanced Shelf Life and Productivity of E. coli Extracts.

Autor: Gregorio NE; Chemistry and Biochemistry Department, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.; Center for Applications in Biotechnology, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States., Kao WY; Chemistry and Biochemistry Department, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.; Center for Applications in Biotechnology, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States., Williams LC; Chemistry and Biochemistry Department, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.; Center for Applications in Biotechnology, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States., Hight CM; Chemistry and Biochemistry Department, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.; Center for Applications in Biotechnology, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States., Patel P; Department of Finance, Orfalea College of Business, California Polytechnic State University, San Luis Obispo, California 93407, United States., Watts KR; Chemistry and Biochemistry Department, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.; Center for Applications in Biotechnology, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States., Oza JP; Chemistry and Biochemistry Department, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.; Center for Applications in Biotechnology, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, United States.

Publikováno v: ACS synthetic biology [ACS Synth Biol] 2020 Apr 17; Vol. 9 (4), pp. 766-778. Date of Electronic Publication: 2020 Mar 12.

Function and Structure of MalA/MalA', Iterative Halogenases for Late-Stage C-H Functionalization of Indole Alkaloids.

Autor: Fraley AE, Garcia-Borràs M; Department of Chemistry and Biochemistry, University of California , Los Angeles, California 90095, United States., Tripathi A, Khare D, Mercado-Marin EV; Department of Chemistry, University of California , Berkeley, California 94720, United States., Tran H, Dan Q, Webb GP, Watts KR; Department of Chemistry and Biochemistry, University of California , Santa Cruz, California 95064, United States., Crews P; Department of Chemistry and Biochemistry, University of California , Santa Cruz, California 95064, United States., Sarpong R; Department of Chemistry, University of California , Berkeley, California 94720, United States., Williams RM; Department of Chemistry, Colorado State University , Fort Collins, Colorado 80523, United States., Smith JL, Houk KN; Department of Chemistry and Biochemistry, University of California , Los Angeles, California 90095, United States., Sherman DH

Publikováno v: Journal of the American Chemical Society [J Am Chem Soc] 2017 Aug 30; Vol. 139 (34), pp. 12060-12068. Date of Electronic Publication: 2017 Aug 21.

Analysis and refactoring of the A-74528 biosynthetic pathway.

Autor: Fitzgerald JT; Department of Chemistry, Stanford University, Stanford, California 94305, USA., Charkoudian LK, Watts KR, Khosla C

Publikováno v: Journal of the American Chemical Society [J Am Chem Soc] 2013 Mar 13; Vol. 135 (10), pp. 3752-5. Date of Electronic Publication: 2013 Mar 04.