Monoamine transporter ubiquitination and inward-open conformation synergistically maximize transporter endocytosis.

Autor: Sorkina T; Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Bagalkot T; Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Cheng MH; Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Guthrie DA; Medicinal Chemistry Section, Molecular Targets and Medications Discovery Branch, National Institute of Drug Abuse-Intramural Research Program, Baltimore, MD, USA., Newman AH; Medicinal Chemistry Section, Molecular Targets and Medications Discovery Branch, National Institute of Drug Abuse-Intramural Research Program, Baltimore, MD, USA., Watkins SC; Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Sorkin A; Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Publikováno v: Science advances [Sci Adv] 2024 Nov 22; Vol. 10 (47), pp. eadq9793. Date of Electronic Publication: 2024 Nov 22.

Drp1 splice variants regulate ovarian cancer mitochondrial dynamics and tumor progression.

Autor: Javed Z; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, PA, USA., Shin DH; Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, PA, USA.; School of Pharmacy, Virginia Commonwealth University, Richmond, VA, USA., Pan W; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., White SR; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Vascular Medicine Institute (VMI), University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Elhaw AT; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, PA, USA., Kim YS; Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, PA, USA.; Division of Human Biology, Fred Hutchinson Cancer Center, Seattle, WA, USA., Kamlapurkar S; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Cheng YY; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Benson JC; Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Abdelnaby AE; Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Phaëton R; Department of Obstetrics & Gynecology, College of Medicine, Pennsylvania State University, Hershey, PA, USA.; GlaxoSmithKline, Collegeville, PA, USA., Wang HG; Department of Pediatrics, College of Medicine, Pennsylvania State University, Hershey, PA, USA., Yang S; Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey, PA, USA., Sullivan MLG; Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., St Croix CM; Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Watkins SC; Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Mullett SJ; Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Health Sciences Mass Spectrometry Core, University of Pittsburgh, Pittsburgh, PA, USA., Gelhaus SL; Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Health Sciences Mass Spectrometry Core, University of Pittsburgh, Pittsburgh, PA, USA., Lee N; Division of Pharmacology, Chemistry and Biochemistry, College of Medicine, University of Arizona, Tucson, AZ, USA., Coffman LG; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Aird KM; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, PA, USA., Trebak M; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, PA, USA.; Vascular Medicine Institute (VMI), University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Mythreye K; Department of Pathology and O'Neal Comprehensive Cancer Center, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA., Walter V; Department of Public Health Sciences, Division of Biostatistics and Bioinformatics and Department of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Hershey, PA, USA., Hempel N; UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. nah158@pitt.edu.; Department of Medicine, Division of Hematology/Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. nah158@pitt.edu.; Vascular Medicine Institute (VMI), University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. nah158@pitt.edu.

Publikováno v: EMBO reports [EMBO Rep] 2024 Oct; Vol. 25 (10), pp. 4281-4310. Date of Electronic Publication: 2024 Aug 27.

A genetically inducible endothelial niche enables vascularization of human kidney organoids with multilineage maturation and emergence of renin expressing cells.

Autor: Maggiore JC; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., LeGraw R; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Przepiorski A; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Velazquez J; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Chaney C; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Vanichapol T; Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand., Streeter E; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Almuallim Z; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Oda A; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Chiba T; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Silva-Barbosa A; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Franks J; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Hislop J; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Hill A; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Wu H; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA., Pfister K; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Howden SE; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Watkins SC; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Little MH; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia., Humphreys BD; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA; Department of Developmental Biology, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA., Kiani S; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Watson A; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Stolz DB; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Davidson AJ; Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand., Carroll T; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Cleaver O; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Sims-Lucas S; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Ebrahimkhani MR; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. Electronic address: mo.ebr@pitt.edu., Hukriede NA; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. Electronic address: hukriede@pitt.edu.

Publikováno v: Kidney international [Kidney Int] 2024 Dec; Vol. 106 (6), pp. 1086-1100. Date of Electronic Publication: 2024 Jun 18.

Comparing ChatGPT and a Single Anesthesiologist's Responses to Common Patient Questions: An Exploratory Cross-Sectional Survey of a Panel of Anesthesiologists.

Autor: Kuo FH; Department of Anesthesia and Pain Medicine, Johns Hopkins All Children's Hospital, 601 5th St South, Suite C725, St Petersburg, FL, 33701, USA. frederick.kuo@jhmi.edu., Fierstein JL; Epidemiology and Biostatistics Shared Resource, Institute for Clinical and Translational Research, Johns Hopkins All Children's Hospital, St Petersburg, FL, USA., Tudor BH; Center for Pediatric Data Science and Analytics Methodology, Johns Hopkins All Children's Hospital, St Petersburg, FL, USA., Gray GM; Center for Pediatric Data Science and Analytics Methodology, Johns Hopkins All Children's Hospital, St Petersburg, FL, USA., Ahumada LM; Center for Pediatric Data Science and Analytics Methodology, Johns Hopkins All Children's Hospital, St Petersburg, FL, USA., Watkins SC; Department of Anesthesia and Pain Medicine, Johns Hopkins All Children's Hospital, 601 5th St South, Suite C725, St Petersburg, FL, 33701, USA., Rehman MA; Department of Anesthesia and Pain Medicine, Johns Hopkins All Children's Hospital, 601 5th St South, Suite C725, St Petersburg, FL, 33701, USA.

Publikováno v: Journal of medical systems [J Med Syst] 2024 Aug 22; Vol. 48 (1), pp. 77. Date of Electronic Publication: 2024 Aug 22.

The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in non-neovascular AMD.

Autor: Ghosh S; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Sharma R; Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, Bethesda, MD, USA., Bammidi S; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Koontz V; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Nemani M; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Yazdankhah M; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Kedziora KM; Department of Cell Biology, Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Stolz DB; Department of Cell Biology, Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Wallace CT; Department of Cell Biology, Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Yu-Wei C; Aging Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Franks J; Department of Cell Biology, Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Bose D; Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, Bethesda, MD, USA., Shang P; Doheny Eye Institute, Pasadena, CA, USA., Ambrosino HM; Doheny Eye Institute, Pasadena, CA, USA., Dutton JR; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA., Geng Z; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA., Montford J; Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, Bethesda, MD, USA., Ryu J; Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, Bethesda, MD, USA., Rajasundaram D; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Hose S; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Sahel JA; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.; Institut De La Vision, INSERM, CNRS, Sorbonne Université, Paris, France., Puertollano R; Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Finkel T; Aging Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Zigler JS Jr; Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA., Sergeev Y; Protein Biochemistry & Molecular Modeling Group, National Eye Institute, National Institutes of Health, Bethesda, MD, USA., Watkins SC; Department of Cell Biology, Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Goetzman ES; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Ferrington DA; Doheny Eye Institute, Pasadena, CA, USA.; Department of Ophthalmology, University of California Los Angeles, Los Angeles, CA, USA., Flores-Bellver M; Department of Ophthalmology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA., Kaarniranta K; Department of Ophthalmology, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland.; Department of Molecular Genetics, University of Lodz, Lodz, Poland., Sodhi A; Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA., Bharti K; Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, Bethesda, MD, USA. kapil.bharti@nih.gov., Handa JT; Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jthanda@jhmi.edu., Sinha D; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Debasish@pitt.edu.; Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. Debasish@pitt.edu.

Publikováno v: Nature communications [Nat Commun] 2024 Jul 21; Vol. 15 (1), pp. 6150. Date of Electronic Publication: 2024 Jul 21.