Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Warren L. Campbell"'
Publikováno v:
Molecular and Cellular Probes. 13:29-33
Mouse hepatitis virus (MHV) infection in laboratory mouse populations is a serious problem, because the MHV infections are known to interfere with research results. Confirmation of indirect serological detection methods by viral isolation is difficul
Publikováno v:
Journal of Rapid Methods and Automation in Microbiology. 5:321-324
A rapid protocol of RT-PCR for the detection of RNA-Virus, Mouse Hepatitis Virus, was developed. An Idaho Air-Thermal Cycler was used with 5 mM Mg CI2 in the buffer so that both RT and PCR steps can be run in one tube and the total detection time is
Autor:
Rong-Fu Wang, Carl E. Cerniglia, Wei-Wen Cao, Latrina Hairston, Warren L. Campbell, Wirt Franklin
Publikováno v:
FEMS Microbiology Letters. 124:229-237
Six PCR primer sets complementary to the 16S rDNAs (rRNA genes) were developed and shown to be specific for the following anaerobic bacteria: Clostridium clostridiiforme, C. perfringens, C. leptum, Bacteroides vulgatus, B. distasonis, and B. thetaiot
Publikováno v:
Journal of Microbiological Methods. 16:239-252
A semicontinuous culture system (SCCS) inoculated with human intestinal bacteria was used to determine if the metabolic activity in the human intestine could be maintained in vitro. After 43 days, a diverse bacterial population was still present with
Publikováno v:
Applied and Environmental Microbiology. 56:661-668
The metabolism of 9-methylanthracene (9-MA), 9-hydroxymethylanthracene (9-OHMA), and 9,10-dimethylanthracene (9,10-DMA) by the fungus Cunninghamella elegans ATCC 36112 is described. The metabolites were isolated by high-performance liquid chromatogra
Autor:
Wirt Franklin, Kenneth L. Dooley, Warren L. Campbell, Carl E. Cerniglia, K. Barry Delclos, Ralph P. Walker
Publikováno v:
Toxicology. 60:137-150
6-Nitrochrysene has previously been shown to be a potent lung and liver carcinogen following i.p. administration to newborn mice and to be metabolically activated to DNA-binding derivatives by nitro-reduction or a combination of nitro-reduction and r
Autor:
Michael F. Bucker, Sean Kelly, Sharon L. Friedman, Donald D. Paine, Steve Madzo, Warren L. Campbell, Michael J. Myers, Denise A. Dove-Pettit, Carl E. Cerniglia, Dorothy E. Farrell, Rong-Fu Wang
Publikováno v:
Journal of food protection. 64(4)
This study validated a polymerase chain reaction-based method for the detection of a specific bovine mitochondrial gene derived from rendered bovine tissues and admixed with complete animal feed. Four laboratories participated in this effort: one sta
Publikováno v:
Current microbiology. 43(2)
A rapid, quantitative method has been developed for determining the tuberculocidal activity of liquid chemical germicides. In this method, a test strain of Mycobacterium bovis that carries the firefly luciferase gene is exposed to a germicide, and th
Autor:
Warren L. Campbell, Michael J. Myers, Wei-Wen Cao, Carl E. Cerniglia, Rong-Fu Wang, Donald D. Paine
Publikováno v:
Molecular and cellular probes. 14(1)
Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in anim
Publikováno v:
Molecular and cellular probes. 8(2)
A 16S rDNA-based polymerase chain reaction (PCR) method was developed for the rapid and specific detection of Clostridium perfringens in food. The PCR primers were designed by a GenBank computer search and they are complementary only with the 16S rRN