Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Wanyu Tao"'
Autor:
Yin Liu, Xinyi Liu, Dongyi Wei, Lu Dang, Xiaoran Xu, Shisheng Huang, Liwen Li, Sanyun Wu, Jinxian Wu, Xiaoyan Liu, Wenjun Sun, Wanyu Tao, Yongchang Wei, Xingxu Huang, Kui Li, Xinjie Wang, Fuling Zhou
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Genetic testing is crucial for precision cancer medicine. However, detecting multiple same-site insertions or deletions (indels) is challenging. Here, we introduce CoHIT (Cas12a-based One-for-all High-speed Isothermal Test), a one-pot CRISPR
Externí odkaz:
https://doaj.org/article/5b17d013b91944509767ea1d1e634457
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Developing computational tools for interpretable cell type annotation in scRNA-seq data remains challenging. Here the authors propose a Transformer-based model for interpretable annotation transfer using biologically understandable entities, and demo
Externí odkaz:
https://doaj.org/article/220f1a6409904b0496a5b2bf54374201
Autor:
Zhenwu Zhang, Wanyu Tao, Shisheng Huang, Wenjun Sun, Yue Wang, Wen Jiang, Xingxu Huang, Chao-Po Lin
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 29, Iss , Pp 502-510 (2022)
Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of
Externí odkaz:
https://doaj.org/article/dc386a3b11744e218cfbac4f06ee4021
Autor:
Xiaofang Cao, Junfan Guo, Shisheng Huang, Wenxia Yu, Guanglei Li, Lisha An, Xiangyang Li, Wanyu Tao, Qing Liu, Xingxu Huang, Xiaohua Jin, Xu Ma
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 28, Iss , Pp 732-742 (2022)
About 47% of pathogenic point mutations could be corrected by ABE-induced A·T-to-G·C conversions. However, the applications of ABEs are still hindered by undesired editing efficiency, limited editing scopes, and off-targeting effects. Here, we deve
Externí odkaz:
https://doaj.org/article/a9cbccc76e424edcbb1a6a5837826a3d
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 2434-2441 (2022)
Gametogenesis, including spermatogenesis and oogenesis, are unique differentiation processes involving extraordinarily complex and precise regulatory mechanisms that require the interactions of multiple cell types, hormones, paracrine factors, genes
Externí odkaz:
https://doaj.org/article/014a2a52b980490489c2df5aecda6b14
CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants
Autor:
Wanyu Tao, Qing Liu, Shisheng Huang, Xin Wang, Shiyuan Qu, Junfan Guo, Danfeng Ou, Guanglei Li, Yu Zhang, Xiangmin Xu, Xingxu Huang
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 114-121 (2021)
Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjac
Externí odkaz:
https://doaj.org/article/1f87b10815b44e5585d50670d4e873ab
Autor:
Bojun Wang, Tiantian Wang, Huimin Zhu, Rong Yan, Xinru Li, Chengqian Zhang, Wanyu Tao, Xisong Ke, Piliang Hao, Yi Qu
Publikováno v:
Cell Reports, Vol 38, Iss 12, Pp 110538- (2022)
Summary: β-Catenin is a central component in the Wnt signaling pathway; its degradation has been tightly connected to ubiquitylation, but it is rarely examined by loss-of-function assays. Here we observe that endogenous β-catenin is not stabilized
Externí odkaz:
https://doaj.org/article/1096bcd5fdec488a9b27510bbef756d2
Autor:
Yajing Liu, Changyang Zhou, Shisheng Huang, Lu Dang, Yu Wei, Jun He, Yingsi Zhou, Shaoshuai Mao, Wanyu Tao, Yu Zhang, Hui Yang, Xingxu Huang, Tian Chi
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.
Externí odkaz:
https://doaj.org/article/f1fdbef8038d4a33ade4dc6a223c19e3
Autor:
Yin Liu, Xinyi Liu, Dongyi Wei, Lu Dang, Xiaoran Xu, Shisheng Huang, Liwen Li, Sanyun Wu, Jinxian Wu, Xiaoyan Liu, Wenjun Sun, Wanyu Tao, Yongchang Wei, Xingxu Huang, Kui Li, Xinjie Wang, Fuling Zhou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7c8f20edacdeb23ee0cef15321ee075b
https://doi.org/10.2139/ssrn.4418705
https://doi.org/10.2139/ssrn.4418705
Publikováno v:
Quantitative Biology. Mar2021, Vol. 9 Issue 1, p23-46. 24p.