Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection.

Autor: Ma J; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada., Tran G; Department of Medical Biophysics, University of Toronto, Toronto, ON, M5G 1L7, Canada., Wan AMD; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, M5S 3G8, Canada., Young EWK; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada.; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, M5S 3G8, Canada., Kumacheva E; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada.; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, M5S 3G8, Canada.; Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada., Iscove NN; Department of Medical Biophysics, University of Toronto, Toronto, ON, M5G 1L7, Canada.; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 1L7, Canada., Zandstra PW; School of Biomedical Engineering, University of British Columbia, 2222 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada. peter.zandstra@ubc.ca.; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. peter.zandstra@ubc.ca.

Publikováno v: Scientific reports [Sci Rep] 2021 Mar 24; Vol. 11 (1), pp. 6777. Date of Electronic Publication: 2021 Mar 24.