Výsledky vyhledávání - "Walt Mahoney"

Akademický článek

Primers with 5′ flaps improve real-time PCR

Autor: Irina Afonina, Irina Ankoudinova, Alan Mills, Sergey Lokhov, Phan Huynh, Walt Mahoney

Publikováno v: BioTechniques, Vol 43, Iss 6, Pp 770-774 (2007)

Primers that contain portions noncomplementary to the target region are usually used to add to the PCR product a utility sequence such as a restriction site or a universal probe binding site. We have demonstrated that primers with short 5′ AT-rich

Externí odkaz: https://doaj.org/article/659d10d4d453400f94ad3ce11ad441d4

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Hepatitis E Virus (HEV) Detection and Quantification by a Real-Time Reverse Transcription-PCR Assay Calibrated to the World Health Organization Standard for HEV RNA

Autor: Jeffrey J. Germer, Chen Dong, Irina Ankoudinova, Jihong Meng, Joseph D. Yao, Yevgeniy S. Belousov, Walt Mahoney, Jayawant N. Mandrekar

Publikováno v: Journal of Clinical Microbiology. 55:1478-1487

Hepatitis E virus (HEV) has emerged as a cause of chronic hepatitis among immunocompromised patients. Molecular assays have become important tools for the diagnosis and management of these chronically infected patients. A real-time reverse transcript

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8219fef1a8b18d0c0be495ced250495
https://doi.org/10.1128/jcm.02334-16

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A rapid, instrument-free, sample-to-result nucleic acid amplification test

Autor: Sujatha Kumar, Lisa Lafleur, Joshua D. Bishop, Xiaohong Zhang, Enos Kline, Peter Kauffman, Barry R. Lutz, Nicolaas M. J. Vermeulen, Ryan Gallagher, Paul Yager, Bhushan J. Toley, Joshua R. Buser, Paula D. Ladd, Noah Scarr, Samantha A. Byrnes, Erin K. Heiniger, Walt Mahoney, Yevgeniy S. Belousov, Maxwell Wheeler

Publikováno v: Lab on a Chip. 16:3777-3787

The prototype demonstrated here is the first fully integrated sample-to-result diagnostic platform for performing nucleic acid amplification tests that requires no permanent instrument or manual sample processing. The multiplexable autonomous disposa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d9d84b985228e821678593d064d76c8
https://doi.org/10.1039/c6lc00677a

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Sensitive and specific assay for the simultaneous detection of Mycoplasma genitalium and macrolide resistance-associated mutations

Autor: Tim T Severs, Yevgeniy S. Belousov, Walt Mahoney, Joyce F Braam, Sebastian van Marm, Johannes G Kusters

Publikováno v: European Journal of Clinical Microbiology and Infectious Diseases, 37(11), 2137-2144. Springer Verlag
Braam, J F, van Marm, S, Severs, T T, Belousov, Y, Mahoney, W & Kusters, J G 2018, ' Sensitive and specific assay for the simultaneous detection of Mycoplasma genitalium and macrolide resistance-associated mutations ', European Journal of Clinical Microbiology and Infectious Diseases, vol. 37, no. 11, pp. 2137-2144 . https://doi.org/10.1007/s10096-018-3350-3

Patients infected by Mycoplasma genitalium are often treated empirically with the macrolide azithromycin. Macrolide resistance is becoming quite common; empirical treatment is compromised. Sequencing was initially used to detected azithromycin resist

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5304b30cd075d5875dbe0d49d334a0e
https://research.vu.nl/en/publications/87ea2da3-6202-4eb1-9e5d-8ede0dc7e9eb

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Sensitive and specific assay for the simultaneous detection of Mycoplasma genitalium and macrolide resistance-associated mutations

Autor: Joyce F, Braam, Sebastian, van Marm, Tim T, Severs, Yevgeniy, Belousov, Walt, Mahoney, Johannes G, Kusters

Publikováno v: European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 37(11)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::94f88ad82e84e8516af0de41f2f76dd3
https://pubmed.ncbi.nlm.nih.gov/30276584

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Malaria prevalence defined by microscopy, antigen detection, DNA amplification and total nucleic acid amplification in a malaria-endemic region during the peak malaria transmission season

Autor: Mitra Singhal, Joseph N. Koros, Jay Gerlach, Samuel B. Anyona, Matt Steele, John N. Waitumbi, Kate Watts, Nicolaas M. J. Vermeulen, Mark E. Polhemus, Irina Ankoudinova, Walt Mahoney, Joram Siangla, Irina A. Afonina, Gonzalo J. Domingo

Publikováno v: Tropical Medicine & International Health. 16:786-793

Summary objectives To determine the malaria prevalence by microscopy, antigen detection and nucleic acid detection in a defined subpopulation in a Plasmodium falciparum–endemic region during the peak transmission season. methods Blood specimens wer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6facc6cd398af7216a60139ac867c808
https://doi.org/10.1111/j.1365-3156.2011.02773.x

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Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus

Autor: Rosemary C. She, Sheri Ferguson, Weston C. Hymas, Alan Mills, Walt Mahoney, Janine Langer, David R. Hillyard

Publikováno v: Journal of Virological Methods. 167:113-118

A high-throughput real-time RT-PCR assay was developed to amplify and detect a conserved region of the hemagglutinin gene of the 2009-H1N1 influenza A virus using a minor groove binder-conjugated hybridization probe. The assay was paired with a separ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f46162e4b78a825f431e039ef2d5d596
https://doi.org/10.1016/j.jviromet.2010.03.020

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Isothermal strand displacement amplification (iSDA): a rapid and sensitive method of nucleic acid amplification for point-of-care diagnosis

Autor: Barry R. Lutz, Enos Kline, Noah Scarr, Paul Yager, Bhushan J. Toley, Isabela Covelli, Nic Vermeulen, Yevgeniy S. Belousov, Walt Mahoney, Sujatha Ramachandran

Publikováno v: The Analyst. 140(22)

We present a method of rapid isothermal amplification of DNA without initial heat denaturation of the template, and methods and probes for (a) real-time fluorescence detection and (b) lateral flow detection of amplicons. Isothermal strand displacemen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae5b623108b5a504a41b5438b05e062a
https://pubmed.ncbi.nlm.nih.gov/26393240

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Improved Biplex Quantitative Real-Time Polymerase Chain Reaction with Modified Primers for Gene Expression Analysis

Autor: Robert O. Dempcy, Sergey G. Lokhov, Silvia Sanders, Walt Mahoney, Irina A. Afonina, Nicolaas M. J. Vermeulen, Alena Kulchenko, Alan Mills

Publikováno v: Oligonucleotides. 16:395-403

Stabilizing modified bases incorporated in primers allows the reduction of housekeeping gene primer concentration not possible with regular primers without sacrificing amplification efficiency. Low primer concentration allows coamplification of the m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d9af32f3cc8960d6fc207c84780cd83
https://doi.org/10.1089/oli.2006.16.395

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Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment

Autor: Silvia Sanders, Irina A. Afonina, Robert Welch, David K. Walburger, Alan Mills, Sunita Yadavalli, Robert O. Dempcy, Alena Kulchenko, Yevgeniy S. Belousov, Cynthia L. Glaser, Walt Mahoney, Nicolaas M. J. Vermeulen

Publikováno v: Human Genomics, Vol 1, Iss 3, Pp 209-217 (2004)
Human Genomics

Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc48d281cd30bda3a5f6859aaf66fa79
http://www.humgenomics.com/content/1/3/209

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