Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Wai Lam W. Ling"'
Autor:
Wai Lam W. Ling
Publikováno v:
Cell Culture Engineering
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0d5cae186621c17a7a5ef8103870d89c
https://doi.org/10.1002/9783527811410.ch11
https://doi.org/10.1002/9783527811410.ch11
Autor:
Xiaona Wei, Shi Ya Mak, Dong-Yup Lee, Dawn Leong, Yee Jiun Kok, Kok Siong Ang, Hsueh Lee Lim, Alison P. Lee, Ying Swan Ho, Shuwen Chen, Say Kong Ng, Taha Salim, Wai Lam W Ling, Neil Templeton, Xuezhi Bi, Andy Hee-Meng Tan, Meiyappan Lakshmanan, Lu Zheng, Eric Gifford
A robust monoclonal antibody (mAb) bioprocess requires physiological parameters such as temperature, pH, or dissolved oxygen (DO) to be well-controlled as even small variations in them could potentially impact the final product quality. For instance,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01c95adbd9bff0d7d72355dd322998f7
https://doi.org/10.22541/au.161476516.60810719/v1
https://doi.org/10.22541/au.161476516.60810719/v1
Publikováno v:
Biotechnology Progress. 31:1163-1171
Advantages of using internally developed chemically-defined (CD) media for cell culture-based therapeutic protein production over commercial media include better raw material control and medium vendor options, and most importantly, flexibility for pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::618795f5317e87fd760772f588d6c38f
https://doi.org/10.1002/btpr.2108
https://doi.org/10.1002/btpr.2108
Publikováno v:
Biotechnology Progress. 29:829-832
This study describes the development work to shorten the monoclonal antibody (mAb) production time in CHO cell cultures from 14 days to 8 days without impacting mAb titer or product quality. The proposed process increases cell inoculation densities u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0071a97ca66295ae9e5c80dcf417f1e2
https://doi.org/10.1002/btpr.1719
https://doi.org/10.1002/btpr.1719
Publikováno v:
Advances in Bioscience and Biotechnology. :918-927
The development and delivery of high quality therapeutic products necessitates the need for highthrough-put (HTP) process development tools. Traditionally, these works requires a combination of shake flask and small-scale stirred tank bioreactor (STR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::97686bd1f271b21ce2df1f0606ee005d
https://doi.org/10.4236/abb.2012.37113
https://doi.org/10.4236/abb.2012.37113
Publikováno v:
Biotechnology Progress. 27:209-219
Chemically defined iron compounds were investigated for the development of animal protein-free cell culture media to support growth of CHO cells and production of monoclonal antibodies (mAb). Using a multivessel approach of 96-well plates, shake flas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::455cfc03e4e1f4602d3da96aaf8c5f85
https://doi.org/10.1002/btpr.513
https://doi.org/10.1002/btpr.513
Autor:
Peter Brams, Yaolin Wang, Deba Saha, Xiaoying Wang, Michael Malkowski, Guanghua Li, Denise Williams, Yan Wang, Lei Xie, Leonard G. Presta, Judith Hailey, Philip Lipari, Robert A. Ramos, Susan Cannon-Carlson, W. Robert Bishop, Robert Greenberg, Jonathan A. Pachter, Wai Lam W. Ling, Robert L. Shields
Publikováno v:
Molecular Cancer Therapeutics. 4:1214-1221
Insulin-like growth factor-I receptor (IGF-IR) plays an important role in tumor cell growth and survival. On ligand stimulation, IGF-IR, a receptor tyrosine kinase, phosphorylates tyrosine residues on two major substrates, IRS-1 and Shc, which subseq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4eec7017329303d48163e3a0004baf00
https://doi.org/10.1158/1535-7163.mct-05-0048
https://doi.org/10.1158/1535-7163.mct-05-0048
Autor:
Liang Deng, Marcio Voloch, Collette Cutler, Susan Cannon-Carlson, Yan Wang, Joseph Lepore, Wai Lam W. Ling
Publikováno v:
Biotechnology Progress. 19:158-162
Hybridoma cultures are routinely used as a source for monoclonal antibody (mAb) production necessary for preclinical evaluation. However, these cultures typically have low volumetric and specific productivities. In this article, we examined the use a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c37ff698908a82825745eff54a38b97
https://doi.org/10.1021/bp020068d
https://doi.org/10.1021/bp020068d
Publikováno v:
Methods (San Diego, Calif.). 16(2)
To investigate the mechanism of secretory granule biogenesis in endocrine cells, our laboratory used rat anterior pituitary GH3 cells which secrete growth hormone and prolactin. Here we describe a simple and rapid procedure for generating permeabiliz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fea42a05b36146231b5b11d66880895
https://pubmed.ncbi.nlm.nih.gov/9790860
https://pubmed.ncbi.nlm.nih.gov/9790860
Autor:
Wai Lam W. Ling, Dennis Shields
The mechanism of secretory-vesicle formation from the trans-Golgi network (TGN) of endocrine cells is poorly understood. To identify cytosolic activities that facilitate the formation and fission of nascent secretory vesicles, we treated permeabilize
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91c8bb23d344f8710ab37f44df684700
https://europepmc.org/articles/PMC1217116/
https://europepmc.org/articles/PMC1217116/