Carbon monoxide binding to α-R277H Mo-nitrogenase - Evidence for multiple pH-dependent species from IR-monitored photolysis

Autor: Leland B. Gee, Aubrey D. Scott, Christie H. Dapper, William E. Newton, Stephen P. Cramer

Publikováno v: Journal of inorganic biochemistry. 232

The nitrogenase (N2ase) enzyme family is responsible for the conversion of dinitrogen into biologically accessible ammonia, a critical step in the global nitrogen cycle. Carbon monoxide (CO) has long been known as an inhibitor of dinitrogen reduction

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6ed26237292d4032b98440a014c0f9e
https://pubmed.ncbi.nlm.nih.gov/35439691

Assignment of protonated R-homocitrate in extracted FeMo-cofactor of nitrogenase via vibrational circular dichroism spectroscopy

Autor: Zhao-Hui Zhou, William E. Newton, Sergey V. Shilov, Lan Deng, Stephen P. Cramer, Shunlin Wang, Christie H. Dapper, Hongxin Wang

Publikováno v: Communications Chemistry, Vol 3, Iss 1, Pp 1-7 (2020)

Protonation of FeMo-cofactor (FeMo-co) is important for the process of substrate hydrogenation. Its structure has been clarified as Δ-Mo*Fe7S9C(R-homocit*)(cys)(Hhis) after the efforts of nearly 30 years, but it remains controversial whether FeMo-co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::404d041de3465b11b83341d06a7731f5
https://doi.org/10.1038/s42004-020-00392-z

Preliminary Assignment of Protonated and Deprotonated Homocitrates in Extracted FeMo-cofactors by Comparisons with Molybdenum(IV) Lactates and Oxidovanadium Glycolates

Autor: Zhao-Hui Zhou, Xing Li, Stephen P. Cramer, William E. Newton, Wan-Ting Jin, Hongxin Wang, Si-Yuan Wang, Christie H. Dapper

Publikováno v: Inorganic chemistry, vol 58, iss 4

A similar pair of protonated and deprotonated mononuclear oxidovanadium glycolates [VO(Hglyc)(phen)(H2O)]Cl·2H2O (1) and [VO(glyc)(bpy)(H2O)] (2) and a mixed-(de)protonated oxidovanadium triglycolate (NH4)2[VO(Hglyc)2(glyc)]·H2O (3) were isolated a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::439abd322741c814b65d91a2997c95df
https://europepmc.org/articles/PMC6813765/

Nitrogen Fixation and Hydrogen Metabolism in Cyanobacteria

Autor: Hermann Bothe, M. Geoffrey Yates, Oliver Schmitz, William E. Newton

Publikováno v: Microbiology and Molecular Biology Reviews. 74:529-551

SUMMARY This review summarizes recent aspects of (di)nitrogen fixation and (di)hydrogen metabolism, with emphasis on cyanobacteria. These organisms possess several types of the enzyme complexes catalyzing N 2 fixation and/or H 2 formation or oxidatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ca7cccf0225a03b98e1ce6f48d85d54
https://doi.org/10.1128/mmbr.00033-10

Low frequency dynamics of the nitrogenase MoFe protein via femtosecond pump probe spectroscopy - Observation of a candidate promoting vibration

Autor: Margherita Maiuri, Yisong Guo, Cristian Manzoni, Leland B. Gee, Giulio Cerullo, Vladimir Pelmenschikov, Stephen P. Cramer, Christie H. Dapper, Ines Delfino, William E. Newton, Hongxin Wang

Publikováno v: Journal of inorganic biochemistry 153 (2015): 128–135. doi:10.1016/j.jinorgbio.2015.07.005
info:cnr-pdr/source/autori:Maiuri, Margherita; Delfino, Ines; Cerullo, Giulio; Manzoni, Cristian; Pelmenschikov, Vladimir; Guo, Yisong; Wang, Hongxin; Gee, Leland B.; Dapper, Christie H.; Newton, William E.; Cramer, Stephen P./titolo:Low frequency dynamics of the nitrogenase MoFe protein via femtosecond pump probe spectroscopy-Observation of a candidate promoting vibration/doi:10.1016%2Fj.jinorgbio.2015.07.005/rivista:Journal of inorganic biochemistry/anno:2015/pagina_da:128/pagina_a:135/intervallo_pagine:128–135/volume:153

We have used femtosecond pump-probe spectroscopy (FPPS) to study the FeMo-cofactor within the nitrogenase (N2ase) MoFe protein from Azotobacter vinelandii. A sub-20-fs visible laser pulse was used to pump the sample to an excited electronic state, an

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https://escholarship.org/uc/item/25c0f06b