Zobrazeno 1 - 10
of 132
pro vyhledávání: '"W.P. van Bennekom"'
Publikováno v:
TrAC Trends in Analytical Chemistry. 45:107-120
In signal transduction and other biological processes, molecular interaction plays an important role. Real-time data on molecular interactions can be helpful in understanding the relationship between occurrence of these processes and binding. Surface
Publikováno v:
Biosensors and Bioelectronics. 24:2184-2190
An automated, on-line system for protein quantification and identification, employing Surface Plasmon Resonance (SPR), enzymatic protein digestion, nanoLC and tandem-MS (MS/MS), has been developed. For the experiments recombinant human interferon-gam
Publikováno v:
Analytical and Bioanalytical Chemistry
A study was initiated to construct a micro-reactor for protein digestion based on trypsin-coated fused-silica capillaries. Initially, surface plasmon resonance was used both for optimization of the surface chemistry applied in the preparation and for
Publikováno v:
Biosensors and Bioelectronics. 21:49-59
The research on our flow-injection, label-free, non-faradaic impedimetric immunosensor for interferon-gamma (IFN-gamma) has been extended. The sensor is prepared by immobilization of anti-IFN-gamma antibodies on a self-assembled monolayer (SAM) of ac
Publikováno v:
Langmuir. 18:3105-3112
The change in characteristics of self-assembled monolayers (SAMs) of thioctic acid (TA) and 11-mercaptoundecanoic acid (MUA) with time in buffered hexacyanoferrate(II/III) solutions of pH 7.4 has been studied with electrochemical impedance spectrosco
Autor:
W.P. van Bennekom, J. C. Hoogvliet
Publikováno v:
Electrochimica Acta. 47:599-611
The effect of the adhesion layer between gold films and quartz on the response of an electrochemical quartz crystal microbalance (EQCM) has been investigated in 0.1 M HClO4 and in 0.1 M phosphate buffer pH 7.4. Cyclic voltammetry (CV) was applied in
Publikováno v:
Langmuir. 16:3852-3857
Formation of self-assembled monolayers (SAMs) of thioctic acid (TA) on polycrystalline gold electrodes has been investigated. The Au electrodes are polished, first mechanically and subsequently electrochemically, until the roughness of the surface is
Publikováno v:
Analytica Chimica Acta, 3, 335, 209-216
Recently a glucose sensor has been developed, which is easily prepared, has a good stability and high glucose response, and is interference free for ascorbic acid. The sensor was made by galvanostatic polymerization of 0.3 M pyrrole in a HEPES buffer
Publikováno v:
Journal of Immunological Methods. 197:161-169
A chemiluminescent substrate reagent for use in a sandwich immunoassay for the model antigen mouse interleukin-5 (IL-5) was developed using xanthine oxidase and luminol. Various parameters involved in this chemiluminescent reaction have been studied,
Autor:
W.P. van Bennekom, T. van Nierop, P. H. Van Der Meide, Auke Bult, H.M. van der Horst, R.F.M. Rombouts, H.A.H. Rongen
Publikováno v:
Analytica Chimica Acta. 306:333-341
In this study parameters are evaluated in order to improve the recently developed liposome immunosorbent assays (LISA) for the cytokines interferon-γ (IFN-γ) and interleukin-2 (IL-2). The first parameter, leakage of the liposomes, is found to be la