Zobrazeno 1 - 10
of 230
pro vyhledávání: '"W.G. Thilly"'
Publikováno v:
BioTechniques, Vol 29, Iss 3, Pp 582-589 (2000)
Constant denaturant capillary electrophoresis (CDCE) has been shown to be a sensitive method to detect point mutations in DNA sequences of 100-bp lengths. Here, we report a significant modifications for the instrumental setup that allows a highly acc
Externí odkaz:
https://doaj.org/article/ae56daeeded34fbda228b52fba77aab4
Publikováno v:
BioTechniques, Vol 27, Iss 1, Pp 128-135 (1999)
We extended our development of the means to measure point mutations at the DNA level to the problem of detecting TP53 gene variants in the area around tumors where mutant fractions are expected to be as low as one mutant per 1000 wild-type (WT) seque
Externí odkaz:
https://doaj.org/article/22970989caf34a628586999989fbdaa0
Autor:
W.G. Thilly, A.R. Oller
Publikováno v:
Journal of Molecular Biology. 228:813-826
To test the hypothesis that reactive species in the oxygen cascade are responsible for spontaneous mutation, we examined the spectra of oxygen and hydrogen peroxide-induced mutations at the hprt locus in a human B-lymphoblastoid cell line. We compare
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b12a9b8896922f44d0b3ff96877fb160
https://doi.org/10.1016/0022-2836(92)90866-i
https://doi.org/10.1016/0022-2836(92)90866-i
Publikováno v:
Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (IEEE Cat. No.03CH37439).
Real-time PCR methods play a valuable role in the characterization of mutations as well as gene expression studies. Existing techniques such as TaqMAN/sup /spl trade// and Molecular Beacons/sup /spl trade// employ specially designed oligonucleotides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0055844c1d74c0a26bb6bebab6f4adfd
https://doi.org/10.1109/iembs.2003.1280787
https://doi.org/10.1109/iembs.2003.1280787
By combining the separation technology of CDCE (constant denaturing capillary electrophoresis) with high fidelity DNA amplification the authors devised a reliable means to measure mutant fractions of any and all point mutations in human cell or tissu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6d9aadaba48fca60ad67221f4ae7dd3d
https://doi.org/10.2172/666235
https://doi.org/10.2172/666235
Autor:
W.G. Thilly
This report discusses measuring methods of point mutations; high density cell cultures for low dose studies; measurement and sequence determination of mutations in DNA; the mutational spectra of styrene oxide and ethlyene oxide in TK-6 cells; mutatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c77dedce291b1fe773a60402d188c72d
https://doi.org/10.2172/10152396
https://doi.org/10.2172/10152396
Autor:
W.G. Thilly
Our goal is to develop the tools of mutational spectrometry in order to discover the cause(s) of genetic change in somatic and germinal cells in humans. Our study of the spectrum of point mutations in human mitochrondrial DNA sequences has revealed t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::76be8165c5d0bf55646d71dbd7d7dd11
https://doi.org/10.2172/10158116
https://doi.org/10.2172/10158116
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