Zobrazeno 1 - 10
of 55
pro vyhledávání: '"W. Wilfinger"'
Publikováno v:
PLoS ONE, Vol 18, Iss 11, p e0291209 (2023)
Numerous methodologies are used for blood RNA extraction, and large quantitative differences in recovered RNA content are reported. We evaluated three archived data sets to determine how extraction methodologies might influence mRNA and lncRNA sequen
Externí odkaz:
https://doaj.org/article/e8dda4681ce241eba309e59d21c63d60
Publikováno v:
BMC Genomics, Vol 22, Iss 1, Pp 1-19 (2021)
Abstract Background RNA sequencing analysis focus on the detection of differential gene expression changes that meet a two-fold minimum change between groups. The variability present in RNA sequencing data may obscure the detection of valuable inform
Externí odkaz:
https://doaj.org/article/ab061a6e5a0b489f915efba04f02f065
Autor:
Piotr Chomczynski, Peter W Chomczynski, Amy Kennedy, Michal Rymaszewski, William W Wilfinger, Judith A Heiny, Karol Mackey
Publikováno v:
PLoS ONE, Vol 16, Iss 2, p e0246867 (2021)
Widespread diagnostic testing is needed to reduce transmission of COVID-19 and manage the pandemic. Effective mass screening requires robust and sensitive tests that reliably detect the SARS-CoV-2 virus, including asymptomatic and pre-symptomatic inf
Externí odkaz:
https://doaj.org/article/fefc16edc817473fa221cd4e7ad7984b
Autor:
Piotr Chomczynski, William W Wilfinger, Hamid R Eghbalnia, Amy Kennedy, Michal Rymaszewski, Karol Mackey
Publikováno v:
PLoS ONE, Vol 11, Iss 2, p e0148260 (2016)
Relatively little is known about the range of RNA levels in human blood. This report provides assessment of peripheral blood RNA level and its inter-individual differences in a group of 35 healthy humans consisting of 25 females and 10 males ranging
Externí odkaz:
https://doaj.org/article/e6202ed379944425a5004bd33268fd15
Publikováno v:
BioTechniques, Vol 22, Iss 3, Pp 474-481 (1997)
The ratio of absorbance at 260 and 280 nm (the A260/280 ratio) is frequently used to assess the purity of RNA and DNA preparations. Data presented in this report demonstrate significant variability in the RNA A260/280 ratio when different sources of
Externí odkaz:
https://doaj.org/article/ccdca100ac444f76a4bb40c211ffe121
Autor:
Karol Mackey, Robert N.G. Miller, Hamid R. Eghbalnia, William W. Wilfinger, Piotr Chomczynski
Publikováno v:
BMC Genomics
BMC Genomics, Vol 22, Iss 1, Pp 1-19 (2021)
BMC Genomics, Vol 22, Iss 1, Pp 1-19 (2021)
Background RNA sequencing analysis focus on the detection of differential gene expression changes that meet a two-fold minimum change between groups. The variability present in RNA sequencing data may obscure the detection of valuable information whe
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports
Scientific Reports
RNA-Seq expression analysis currently relies primarily upon exon expression data. The recognized role of introns during translation, and the presence of substantial RNA-Seq counts attributable to introns, provide the rationale for the simultaneous co
Publikováno v:
eLS
The original single-step method is the first procedure to isolate purified total ribonucleic acid (RNA) from a variety of sources including tissues and cells from human, animal, plant, yeast, bacterial and viral origins, without the requirement of hi
Autor:
Hamid R. Eghbalnia, Piotr Chomczynski, Karol Mackey, Michal Rymaszewski, William W. Wilfinger, Amy Kennedy
Publikováno v:
PLoS ONE, Vol 11, Iss 2, p e0148260 (2016)
PLoS ONE
PLoS ONE
Relatively little is known about the range of RNA levels in human blood. This report provides assessment of peripheral blood RNA level and its inter-individual differences in a group of 35 healthy humans consisting of 25 females and 10 males ranging
Autor:
J Billiani, W Wilfinger
Publikováno v:
Surface Coatings International Part B: Coatings Transactions. 85:191-195
Two-pack water-borne polyurethane coatings are a relatively new technology which promises excellent performance properties combined with a low content of volatile organic compounds (VOCs). In this paper the effects of a polymer structure of acrylic p