Zobrazeno 1 - 10
of 14
pro vyhledávání: '"W. Michael Holmes"'
Publikováno v:
Biochemistry. 43:9243-9255
A human TRM5 cDNA has been cloned and recombinant tRNA-N(1)G37 methyltransferase was produced. The recombinant enzyme methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts utilizing S-adenosyl methionine. The effects of RNA se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::531c67d5ad20c28419f8ff8a6d9059af
https://doi.org/10.1021/bi049671q
https://doi.org/10.1021/bi049671q
Autor:
Anirban Chowdhury, Vineeta A. Pradhan, Ailong Ke, Tina M. Henkin, Fang Ding, W. Michael Holmes, Changrui Lu, Jerneja Tomsic
Publikováno v:
Journal of molecular biology. 404(5)
S-box (SAM-I) riboswitches are a widespread class of riboswitches involved in the regulation of sulfur metabolism in Gram-positive bacteria. We report here the 3.0-A crystal structure of the aptamer domain of the Bacillus subtilis yitJ S-box (SAM-I)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80fc41d3b13420f6e42dbb6ce4f2f41f
https://pubmed.ncbi.nlm.nih.gov/20951706
https://pubmed.ncbi.nlm.nih.gov/20951706
Publikováno v:
Protein Expression and Purification. 2:117-121
In an Escherichia coli expression system, two genes, one from an anaerobic intestinal bacterium and one from E. coli, were overexpressed following the alteration of ribosome-binding (Shine-Dalgarno) sequences. For both genes, the polymerase chain rea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a50ae0c26195e3d267ab0be1e021ace8
https://doi.org/10.1016/1046-5928(91)90059-r
https://doi.org/10.1016/1046-5928(91)90059-r
Autor:
Zendra E. Zehner, May Al‐Maghrebi, Marina Padkina, Carrie Allen, Hervé Brulé, W. Michael Holmes
Previously, we have shown that the vimentin 3' untranslated region (3'UTR) contains a highly conserved region, which is sufficient for the perinuclear localization of a reporter mRNA. This region was shown to specifically bind protein(s) by band shif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab28892a00c26f52f8e0bd437beae93d
https://europepmc.org/articles/PMC137969/
https://europepmc.org/articles/PMC137969/
Autor:
Rebecca K. Shepherd, Zendra E. Zehner, W. Michael Holmes, Tzu Fun Fu, Mav Al-Ali, Joanna Gabryszuk
Publikováno v:
Nucleic acids research. 25(16)
Several functions have been attributed to protein binding within the 3'untranslated region (3'UTR) of mRNA, including mRNA localization, stability, and translational repression. Vimentin is an intermediate filament protein whose 3'untranslated sequen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::321ab78d0c15cc67514f7a223f6ca4d9
https://pubmed.ncbi.nlm.nih.gov/9241253
https://pubmed.ncbi.nlm.nih.gov/9241253
Publikováno v:
Cell. 32:1029-1032
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b0fd71b1387bcf0a28a8f99a5d700db
https://doi.org/10.1016/0092-8674(83)90287-8
https://doi.org/10.1016/0092-8674(83)90287-8
Publikováno v:
Cell. 30:855-864
We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53a8f06f064dd05bba8093937ee42f83
https://doi.org/10.1016/0092-8674(82)90290-2
https://doi.org/10.1016/0092-8674(82)90290-2
Publikováno v:
Nucleic Acids Research. 9:2121-2140
Fourteen different DNA fragments containing Escherichia coli tRNA genes have been cloned using the vector pBR322. We report the methods of cloning, the identification of specific tRNA genes, and the presence or absence of rRNA genes on these cloned D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b286f381e2bf8c2ec4c6d70b86fad32
https://doi.org/10.1093/nar/9.9.2121
https://doi.org/10.1093/nar/9.9.2121
Publikováno v:
Gene. 77(2)
An analysis of 5' and 3' deletions of the human tRNAiMet3 gene has revealed upstream regions required for efficient transcription and stable complex formation in vitro. The 5' boundary of this essential region lies between nucleotides -39 to -18 (sta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c10cbe49a0f1c241960a09903db348c2
https://pubmed.ncbi.nlm.nih.gov/2753363
https://pubmed.ncbi.nlm.nih.gov/2753363
Publikováno v:
Gene. 81:193-194
A 300-nucleotide sequence was determined which includes the tRNALeu3coding region and the flanking sequences.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78e721db766f34c0e86021ede3d1f364
https://doi.org/10.1016/0378-1119(89)90351-x
https://doi.org/10.1016/0378-1119(89)90351-x