Zobrazeno 1 - 10
of 89
pro vyhledávání: '"W. McLean Grogan"'
Publikováno v:
Journal of Lipid Research, Vol 40, Iss 11, Pp 2091-2098 (1999)
Expression of the rat liver neutral cytosolic cholesteryl ester hydrolase (CEH) gene is regulated by glucocorticoids, thyroxine, and agents that perturb cholesterol metabolism. The present studies identify the putative hormone response elements in th
Externí odkaz:
https://doaj.org/article/ac6a1f65413d4d3bb13700c323c8185a
Publikováno v:
Europe PubMed Central
To understand molecular events in regulation of hepatic neutral cholesteryl ester hydrolase (EC3.1.1.13; CEH), catalytic activity, protein mass, and mRNA levels were measured in rats with various perturbations of hepatic cholesterol metabolism. Chole
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1259:305-312
The 1923 bp cDNA for rat hepatic cholesteryl ester hydrolase (CEH) was cloned by screening a λgt11 expression library with an oligonucleotide containing the consensus active site sequence for cholesteryl esterases. Expression of a fusion protein, cr
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1182:152-156
Excess 6 beta-OH-corticosterone production by family 3A cytochromes P-450 may play a role in genesis of hypertension in the spontaneously hypertensive rat (SHR), by producing a renal defect in Na+ excretion. Renal cytochromes P-450 may be a causal fa
Autor:
W. McLean Grogan, Shobha Ghosh
Publikováno v:
Biochemistry and Cell Biology. 70:800-803
Rabbit polyclonal antibodies were raised against rat liver bile salt-independent neutral cholesteryl ester hydrolase (CEH) and used for subcellular localization and immunological comparison with isoforms from other tissues. Antibodies exhibited a hig
Publikováno v:
Experimental Cell Research. 196:192-197
Rigidity of the outer hemileaflet of the plasma membrane of two prostatic carcinoma cell lines with different metastatic potential, 1-LN and 1-LN-EMS-10, was assessed by steady-state anisotropy, using a battery of fluorescent probes. The "bulk" membr
Autor:
Shobha Ghosh, W. McLean Grogan
Publikováno v:
Lipids. 26:793-798
A rat liver cytosolic cholesteryl ester hydrolase (CEH) was purified 12,600-fold by ammonium sulfate precipitation, cation exchange chromatography and gel permeation high-performance liquid chromatography, with an overall yield of 20%. Its properties
Autor:
James M. Collins, W. McLean Grogan
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1067:171-176
We have used an extended Perrin equation which was in agreement with literature data for steady-state anisotropy (rSS) for a wide variety of artificial and isolated biological membranes labeled with various probes (Van der Meer et al. (1986) Biochim.
Publikováno v:
Journal of Cellular Physiology. 144:42-51
The shape of the fluidity gradient of the outer hemileaflet of the plasma membrane of normal, living, human white blood cells was determined using a series of n-(9-anthroyloxy) fatty acid probes where n = 2, 3, 6, 7, 9, 11, 12, and 16, to establish a
Publikováno v:
American journal of respiratory cell and molecular biology. 20(6)
The 1,839-base pair complementary DNA (cDNA) for rat lung carboxylesterase was cloned by reverse transcriptase polymerase chain reaction from total rat lung RNA using specific primers derived from the 5' and 3' untranslated regions of rat hepatic cho