Variable orthogonality of serine integrase interactions within the ϕC31 family

Autor: Alasdair I. MacDonald, Aron Baksh, Alexandria Holland, Heewhan Shin, Phoebe A. Rice, W. Marshall Stark, Femi J. Olorunniji

Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)

Abstract Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integratin

Externí odkaz: https://doaj.org/article/73b6640b58ef469a864e4d5cbc87a337

High fidelity one-pot DNA assembly using orthogonal serine integrases

Autor: Jumai Abioye, Makeba Lawson‐Williams, Alicia Lecanda, Brecken Calhoon, Arlene L. McQue, Sean D. Colloms, W. Marshall Stark, Femi J. Olorunniji

Publikováno v: Biotechnology journal.

Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called Serine Integrase Recombinational Assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is limite

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae32c22bc7f726c6fe645871feb586a2
https://pubmed.ncbi.nlm.nih.gov/36504358

Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending

Autor: Aleksandra Bebel, Ezgi Karaca, Banushree Kumar, W Marshall Stark, Orsolya Barabas

Publikováno v: eLife, Vol 5 (2016)

Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicoba

Externí odkaz: https://doaj.org/article/cc02f1a8843444c5a9a72ff87df08c89

Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing

Autor: Jane E. Paget, Femi J. Olorunniji, Susan J. Rosser, Arlene L. McPherson, W. Marshall Stark, Makeba Lawson-Williams

Publikováno v: Nucleic Acids Research
Olorunniji, F J, Lawson-Williams, M, Mcpherson, A L, Paget, J E, Stark, W M & Rosser, S J 2019, ' Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing ', Nucleic Acids Research, vol. 47, no. 21, pp. 11452-11460 . https://doi.org/10.1093/nar/gkz936

Serine integrases are emerging as core tools in synthetic biology and have applications in biotechnology and genome engineering. We have designed a split-intein serine integrase-based system with potential for regulation of site-specific recombinatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74601333b4036566283ade5d654ca48d
http://europepmc.org/articles/PMC6868429

Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

Autor: Janina Hossbach, Hong Gao, Stephanie Evans, Margaret C. M. Smith, Buvani Murugesan, W. Marshall Stark

Publikováno v: BMC Biotechnology
BMC Biotechnology, Vol 19, Iss 1, Pp 1-10 (2019)

Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify integrating vectors, derived using phageint

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03610d81ac1d6bffc67d5195ec669f6b
https://pubmed.ncbi.nlm.nih.gov/31164159