Výsledky vyhledávání - "W. J. M. Spaan"

Rhinovirus inhalation causes long-lasting excessive airway narrowing in response to methacholine in asthmatic subjects in vivo

Autor: W. J. M. Spaan, E. P. A. De Klerk, E. C. Dick, P. J. Sterk, M. C. Timmers, D. Cheung

Publikováno v: American journal of respiratory and critical care medicine, 152(5 Part 1), 1490-1496. American Thoracic Society

Exacerbations of asthma are often associated with respiratory infections, and particularly those caused by rhinovirus. The causative role of rhinovirus in these acute episodes is still unclear, since it has not been determined whether or not infectio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be43feca2c63fc043817f9428b2375b7
https://doi.org/10.1164/ajrccm.152.5.7582282

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Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation

Autor: W. J. M. Spaan, R. G. Van Der Most, R. J. de Groot

Publikováno v: Journal of Virology. 68:3656-3666

We have used a full-length cDNA clone of a mouse hepatitis virus strain A59 defective interfering (DI) RNA, pMIDI-C, and cassette mutagenesis to study the mechanism of coronavirus subgenomic mRNA synthesis. Promoter sequences closely resembling those

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fd2abce6b2712e72a7660de87d2a504
https://doi.org/10.1128/jvi.68.6.3656-3666.1994

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Gene mapping and expression of two immunodominant Epstein-Barr virus capsid proteins

Autor: Jaap M. Middeldorp, E. C. Van Heerde, W. J. M. Spaan, H. J. W. De Haard, W. M. J. Van Grunsven

Publikováno v: Europe PubMed Central

The genomic localization of two immunodominant genes encoding two proteins of the Epstein-Barr virus capsid antigen (VCA) complex, VCA-p18 and VCA-p40, has been identified. For that purpose, lambda gt11-based cDNA libraries were constructed from HH51

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8074bdfb38a1b817a4b9f1a20527517b
https://doi.org/10.1128/jvi.67.7.3908-3916.1993

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Intracellular transport of recombinant coronavirus spike proteins: implications for virus assembly

Autor: W. J. M. Spaan, Leo Heijnen, Harry Vennema, Marian C. Horzinek, A. J. Zijderveld

Publikováno v: Journal of virology, 64(1), 339. American Society for Microbiology

Coronavirus spike protein genes were expressed in vitro by using the recombinant vaccinia virus expression system. Recombinant spike proteins were expressed at the cell surface and induced cell fusion in a host-cell-dependent fashion. The intracellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d90cc3a3e14962e676261fd6a494bb9
https://doi.org/10.1128/jvi.64.1.339-346.1990

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Effect of experimental rhinovirus 16 colds on airway hyperresponsiveness to histamine and interleukin-8 in nasal lavage in asthmatic subjects in vivo

Autor: Hermelijn H. Smits, Peter J. Sterk, E. P. A. De Klerk, Katrien Grünberg, W. J. M. Spaan, Pieter S. Hiemstra, M. C. Timmers, E. C. Dick

Publikováno v: Clinical and experimental allergy, 27(1), 36-45. Wiley-Blackwell
Clinical and Experimental Allergy

BACKGROUND: Asthma exacerbations are closely associated with respiratory virus infections. However, the pathophysiological consequences of such infections in asthma are largely unclear. OBJECTIVE: To examine the effect of rhinovirus 16 (RV16) infecti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b2af059084778090b72edcd98973903
https://pure.amc.nl/en/publications/effect-of-experimental-rhinovirus-16-colds-on-airway-hyperresponsiveness-to-histamine-and-interleukin8-in-nasal-lavage-in-asthmatic-subjects-in-vivo(2b61dc60-8aaf-4618-8af2-73d04d41bf8f).html

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The Coronaviridae Now Comprises Two Genera, Coronavirus and Torovirus: Report of the Coronaviridae Study Group

Autor: D. Cavanagh, D. A. Brian, M. A. Brinton, L. Enjuanes, K. V. Holmes, M. C. Horzinek, M. M. C. Lai, H. Laude, P. G. W. Plagemann, S. G. Siddell, W. J. M. Spaan, F. Taguchi, P. J. Talbot

Publikováno v: Coronaviruses ISBN: 9781461363057

At the April 1992, mid-term meeting of the International Committee on Taxonomy of Viruses (ICTV) a proposal from the Coronaviridae Study Group (CSG) to include the torovirus genus in the Coronaviridae was accepted. Following another proposal, the art

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a27606826f2b73fdf5eca38481885653
https://doi.org/10.1007/978-1-4615-2996-5_39

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Homologous RNA recombination allows efficient introduction of site-specific mutations into the genome of coronavirus MHV-A59 via synthetic co-replicating RNAs

Autor: Leo Heijnen, de Groot R, W. J. M. Spaan, van der Most R

Publikováno v: Positive-Strand RNA Viruses ISBN: 9783211825228

We describe a novel strategy to site-specifically mutagenize the genome of an RNA virus by exploiting homologous RNA recombination between synthetic defective interfering (DI) RNA and viral RNA. Marker mutations introduced in the DI RNA were replaced

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2205121a399b5b11daf6d716fbb00dfb
https://doi.org/10.1007/978-3-7091-9326-6_22

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Immunogenic peptide comprising a mouse hepatitis virus A59 B-cell epitope and an influenza virus T-cell epitope protects against lethal infection

Autor: Marck J. M. Koolen, Marian C. Horzinek, M. A. J. Borst, W. J. M. Spaan

The coronavirus spike protein S is responsible for important biological activities including virus neutralization by antibody, cell attachment, and cell fusion. Recently, we have elucidated the amino acid sequence of an S determinant common in murine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fddcd314fdc51c9053c31b73968afb46
https://europepmc.org/articles/PMC248803/

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Reverse transcription and cDNA amplification by the polymerase chain reaction of equine arteritis virus (EAV)

Autor: E. D. Chirnside, W. J. M. Spaan

Publikováno v: Journal of virological methods. 30(2)

A technique is described for the amplification and specific identification of equine arteritis virus (EAV) nucleotide sequences. The polymerase chain reaction (PCR) was evaluated initially by amplification of cloned virus specific cDNA sequences prio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12c650b143fad8467ecc93ac36f530d8
https://pubmed.ncbi.nlm.nih.gov/1702094

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