Výsledky vyhledávání - "W. G. Dougherty"

Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus

Autor: W. G. Dougherty, K. E. Keller, R. O. Hampton, I. E. Johansen

Publikováno v: Johansen, I E, Keller, K E, Dougherty, W G & Hampton, R O 1996, ' Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus ' Journal of General Virology, vol. 77, pp. 1329-1333 .

Two isolates of pea seed-borne mosaic potyvirus, DPD1 and NY, were identified as pathotypes P-1 and P-4, respectively, by their infectivity on Pisum sativum L. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. The two isolates diff

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https://doi.org/10.1099/0022-1317-77-6-1329

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Genetic and biochemical dissection of transgenic RNA-mediated virus resistance

Autor: E A Wernsman, T D Parks, K Chapman, W G Dougherty, J Goodwin, S Swaney

Publikováno v: The Plant Cell. 8:95-105

RNA-mediated virus resistance has been observed in transgenic plants at varying frequencies, suggesting that a nuclear requirement or other pre-condition must be met. This study was undertaken to characterize genetically transgenes that confer a high

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https://doi.org/10.1105/tpc.8.1.95

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Expression and Purification of a Recombinant Tobacco Etch Virus NIa Proteinase: Biochemical Analyses of the Full-Length and a Naturally Occurring Truncated Proteinase Form

Autor: D J Arp, T J Wolpert, W G Dougherty, T D Parks, E.D. Howard

Publikováno v: Virology. 210:194-201

The tobacco etch virus 27-kDa nuclear inclusion a (NIa) proteinase was expressed in Escherichia coli as a recombinant fusion protein containing a seven-histidine tag at the amino-terminus. Catalytically active and inactive (by virtue of a single amin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5dd2230b4b4d8b696d62862a056dae5
https://doi.org/10.1006/viro.1995.1331

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Transgene and gene suppression: telling us something new?

Autor: T D Parks, W G Dougherty

Publikováno v: Current Opinion in Cell Biology. 7:399-405

Transgenes provide unique opportunities to assess the relationship between genotype and phenotype in an organism. In most cases, introduction and subsequent expression of a transgene will increase (with a sense RNA) or decrease (with an antisense RNA

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https://doi.org/10.1016/0955-0674(95)80096-4

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Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs

Autor: T D Parks, H A Smith, W G Dougherty, S Swaney, E A Wernsman

Publikováno v: The Plant Cell. 6:1441-1453

Haploid leaf tissue of tobacco cultivars K326 and K149 was transformed with several transgenes containing cDNA of the potato virus Y (PVY) coat protein (CP) open reading frame (ORF). The various transgenes containing the PVY CP ORF sequence produced

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https://doi.org/10.1105/tpc.6.10.1441

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Application of TEV Protease in Protein Production

Autor: D A, Polayes, T D, Parks, S A, Johnston, W G, Dougherty

Publikováno v: Methods in molecular medicine. 13

In many cases, the analysis of a specific protein is impeded by the inability to purify large amounts of it from a native source. Proteins of interest may be present in minute quantities and/or purification may be plagued with technical problems. Rec

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https://pubmed.ncbi.nlm.nih.gov/21390844

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Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance

Autor: William M. Proebsting, Laura Silva-Rosales, John A. Lindbo, W G Dougherty

Publikováno v: The Plant Cell. :1749-1759

Transgenic tobacco plants expressing either a full-length form of the tobacco etch virus (TEV) coat protein or a form truncated at the N terminus of the TEV coat protein were initially susceptible to TEV infection, and typical systemic symptoms devel

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https://doi.org/10.1105/tpc.5.12.1749

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Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes

Autor: W G Dougherty, Bert L. Semler

Publikováno v: Microbiological Reviews. 57:781-822

Many viruses express their genome, or part of their genome, initially as a polyprotein precursor that undergoes proteolytic processing. Molecular genetic analyses of viral gene expression have revealed that many of these processing events are mediate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42c6a0c696d2a237f85259956236695e
https://doi.org/10.1128/mr.57.4.781-822.1993

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Pathogen derived resistance to potyviruses: working, but why?

Autor: Laura Silva-Rosales, John A. Lindbo, W G Dougherty

Publikováno v: Seminars in Virology. 4:369-379

The theory of pathogen-derived resistance suggests that pathogen resistance genes can be developed from a pathogen's own genetic material. For example, transgenic plants expressing plant virus coat protein genes are often virus resistant. Non-structu

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https://doi.org/10.1006/smvy.1993.1036

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