Zobrazeno 1 - 10
of 208
pro vyhledávání: '"W. David Nes"'
Publikováno v:
Biomolecules, Vol 14, Iss 3, p 249 (2024)
Sterol biosynthesis via the mevalonate-isoprenoid pathway produces ergosterol (24β-methyl cholesta-5,7-dienol) necessary for growth in a wide-range of eukaryotic pathogenic organisms in eukaryotes, including the fungi, trypanosomes and amoebae, whil
Externí odkaz:
https://doaj.org/article/32268203d6b44c92945cf415e8ba7261
Autor:
Wenxu Zhou, Paxtyn M. Fisher, Boden H. Vanderloop, Yun Shen, Huazhong Shi, Adrian J. Maldonado, David J. Leaver, W. David Nes
Publikováno v:
Journal of Lipid Research, Vol 61, Iss 2, Pp 192-204 (2020)
Primitive sterol evolution plays an important role in fossil record interpretation and offers potential therapeutic avenues for human disease resulting from nematode infections. Recognizing that C4-methyl stenol products [8(14)-lophenol] can be synth
Externí odkaz:
https://doaj.org/article/02378f2c8d70426382e780781511bfb5
Publikováno v:
Molecules, Vol 27, Iss 13, p 4088 (2022)
Trypanosoma brucei, the causative agent for human African trypanosomiasis, is an emerging ergosterol-dependent parasite that produces chokepoint enzymes, sterol methyltransferases (SMT), not synthesized in their animal hosts that can regulate cell vi
Externí odkaz:
https://doaj.org/article/a81ef89711f1474fa8ac344486fdba14
Autor:
Medhanie E. Kidane, Boden H. Vanderloop, Wenxu Zhou, Crista D. Thomas, Emilio Ramos, Ujjal Singha, Minu Chaudhuri, W. David Nes
Publikováno v:
Journal of Lipid Research, Vol 58, Iss 12, Pp 2310-2323 (2017)
Ergosterol biosynthesis pathways essential to pathogenic protozoa growth and absent from the human host offer new chokepoint targets. Here, we present characterization and cell-based interference of Acanthamoeba spp sterol 24-/28-methylases (SMTs) th
Externí odkaz:
https://doaj.org/article/c5dd241f6f744dcebd1bd6c255049167
Autor:
Brad A. Haubrich, Ujjal K. Singha, Matthew B. Miller, Craigen R. Nes, Hosanna Anyatonwu, Laurence Lecordier, Presheet Patkar, David J. Leaver, Fernando Villalta, Benoit Vanhollebeke, Minu Chaudhuri, W. David Nes
Publikováno v:
Journal of Lipid Research, Vol 56, Iss 2, Pp 331-341 (2015)
Ergosterol biosynthesis and homeostasis in the parasitic protozoan Trypanosoma brucei was analyzed by RNAi silencing and inhibition of sterol C24β-methyltransferase (TbSMT) and sterol 14α-demethylase [TbSDM (TbCYP51)] to explore the functions of st
Externí odkaz:
https://doaj.org/article/969e699d53874ba9bbc88e64c685f558
Publikováno v:
Journal of Lipid Research, Vol 53, Iss 8, Pp 1636-1645 (2012)
Ergosterol is the predominant sterol of fungi and green algae. Although the biosynthetic pathway for sterol synthesis in fungi is well established and is known to use C24-methylation-C24 (28)-reduction (Δ24(28)-olefin pathway) steps, little is known
Externí odkaz:
https://doaj.org/article/6e297192a7e04572b7e95013dd0b65a9
Autor:
Tatiana Y. Hargrove, Zdzislaw Wawrzak, Jialin Liu, Michael R. Waterman, W. David Nes, Galina I. Lepesheva
Publikováno v:
Journal of Lipid Research, Vol 53, Iss 2, Pp 311-320 (2012)
Sterol 14α-demethylase (CYP51) that catalyzes the removal of the 14α-methyl group from the sterol nucleus is an essential enzyme in sterol biosynthesis, a primary target for clinical and agricultural antifungal azoles and an emerging target for ant
Externí odkaz:
https://doaj.org/article/c40939c9d3c346a897504b45a1c2974f
Publikováno v:
Journal of Lipid Research, Vol 48, Iss 3, Pp 665-673 (2007)
Trypanosoma brucei (TB) cultured in rat blood, bovine serum, or lipid-depleted serum generated distinct differences in cholesterol availability. Whereas cell proliferation of the parasite was relatively unaffected by cholesterol availability, the rat
Externí odkaz:
https://doaj.org/article/ddd9069ac04647bb8068e3b2c038c662
Autor:
W. David Nes, Mathew B. Miller, Phani Veeramachanemi, Archana Sinha, Pruthvi Jayasimha, Jialin Liu, Ragu Kanagasabai, Zhihong Song, Wenxu Zhou
Publikováno v:
Molecules, Vol 9, Iss 4, Pp 185-203 (2004)
Current progress on the mechanism and substrate recognition by sterol methyl transferase (SMT), the role of mechanism-based inactivators, other inhibitors of SMT action to probe catalysis and phytosterol synthesis is reported. SMT is a membrane-bound
Externí odkaz:
https://doaj.org/article/c514f08e6352436481d3128658bd0518
Publikováno v:
Journal of Lipid Research, Vol 42, Iss 1, Pp 128-136 (2001)
Sterol 14α-demethylase (14DM) is a cytochrome P-450 involved in sterol biosynthesis in eukaryotes. It was reported that Mycobacterium smegmatis also makes cholesterol and that cholesterol is essential to Mycobacterium tuberculosis (MT) infection, al
Externí odkaz:
https://doaj.org/article/6cfd01dfbef142c888507b1d467b8012