Výsledky vyhledávání

Evidence that the conserved region in the steroid binding domain of the glucocorticoid receptor is required for both optimal binding of hsp90 and protection from proteolytic cleavage. A two-site model for hsp90 binding to the steroid binding domain

Autor: E R Sanchez, G M Ringold, Paul R. Housley, Mark Danielsen, W B Pratt

Publikováno v: Journal of Biological Chemistry. 265:12778-12781

Steroid hormone receptors contain a conserved sequence of amino acids within the steroid binding domain, and we have previously speculated that this conserved region is the site of interaction of the glucocorticoid receptor with hsp90 (Danielsen, M.,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4997ea226e7ebd31701563b6d010e438
https://doi.org/10.1016/s0021-9258(19)38225-0

Zobrazit plný text záznamu

Chaperoning of glucocorticoid receptors

Autor: W B, Pratt, Y, Morishima, M, Murphy, M, Harrell

Publikováno v: Handbook of experimental pharmacology. (172)

A multiprotein hsp90/hsp70-based chaperone machinery functions as a 'cradle-to-grave' system for regulating the steroid binding, trafficking and turnover of the glucocorticoid receptor (GR). In an ATP-dependent process where hsp70 and hsp90 act as es

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c817633a17750a9d8d1ba8c275af1cf0
https://pubmed.ncbi.nlm.nih.gov/16610357

Zobrazit plný text záznamu

Incidence of hip fracture in Alaska Inuit people: 1979-89 and 1996-99

Autor: W B, Pratt, J M, Holloway

Publikováno v: Alaska medicine. 43(1)

Review of records of the Alaska Native Medical Center in Anchorage, Alaska show that the incidence of hip fracture in Inuit women over 64 years of age was higher than the incidence in white US women in 1979-89 and had increased farther by 1996-99. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ffe0da23a857b8fd254505b18a380f1d
https://pubmed.ncbi.nlm.nih.gov/11345855

Zobrazit plný text záznamu

Evidence for iterative ratcheting of receptor-bound hsp70 between its ATP and ADP conformations during assembly of glucocorticoid receptor.hsp90 heterocomplexes

Autor: Y, Morishima, K C, Kanelakis, P J, Murphy, D S, Shewach, W B, Pratt

Publikováno v: Biochemistry. 40(4)

hsp90 and hsp70 are essential components of a five-protein system, including also the nonessential cochaperones Hop, hsp40, and p23, that assembles glucocorticoid receptor (GR).hsp90 heterocomplexes and causes the simultaneous opening of the steroid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f281ebdbb1e8e370821b6b9e9aad1e19
https://pubmed.ncbi.nlm.nih.gov/11170435

Zobrazit plný text záznamu

p50(cdc37) binds directly to the catalytic domain of Raf as well as to a site on hsp90 that is topologically adjacent to the tetratricopeptide repeat binding site

Autor: A M, Silverstein, N, Grammatikakis, B H, Cochran, M, Chinkers, W B, Pratt

Publikováno v: The Journal of biological chemistry. 273(32)

Several protein kinases (e.g. pp60(src), v-Raf) exist in heterocomplexes with hsp90 and a 50-kDa protein that is the mammalian homolog of the yeast cell cycle control protein Cdc37. In contrast, unliganded steroid receptors exist in heterocomplexes w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1cb91ebcf0077f4dd39c2ff8e7b80748
https://pubmed.ncbi.nlm.nih.gov/9685350

Zobrazit plný text záznamu

Folding of the glucocorticoid receptor by the heat shock protein (hsp) 90-based chaperone machinery. The role of p23 is to stabilize receptor.hsp90 heterocomplexes formed by hsp90.p60.hsp70

Autor: K D, Dittmar, D R, Demady, L F, Stancato, P, Krishna, W B, Pratt

Publikováno v: The Journal of biological chemistry. 272(34)

In cytosols from animal and plant cells, the abundant heat shock protein hsp90 is associated with several proteins that act together to assemble steroid receptors into receptor.hsp90 heterocomplexes. We have reconstituted a minimal receptor.hsp90 ass

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d81059d9bf5b5151c8354b698195fe5e
https://pubmed.ncbi.nlm.nih.gov/9261129

Zobrazit plný text záznamu

Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation

Autor: K D, Dittmar, W B, Pratt

Publikováno v: The Journal of biological chemistry. 272(20)

Rabbit reticulocyte lysate contains a multiprotein chaperone system that assembles steroid receptors into a complex with hsp90. The glucocorticoid receptor (GR) is bound to hsp90 via its hormone binding domain (HBD), which must be associated with hsp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::55c080a7461ac211e9660084c4c7e8b5
https://pubmed.ncbi.nlm.nih.gov/9148915

Zobrazit plný text záznamu

Immunofluorescence localization of the 90-kDa heat-shock protein to cytoskeleton

Autor: M J, Czar, M J, Welsh, W B, Pratt

Publikováno v: European journal of cell biology. 70(4)

The 90-kDa heat-shock protein, hsp90, is an abundant and conserved protein located predominantly in the cytoplasm. Previous reports have localized a portion of the hsp90 to microtubules and to microfilaments. Here, we show that colocalization of hsp9

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e7ad17a128901c1da69203c4220868a0
https://pubmed.ncbi.nlm.nih.gov/8864660

Zobrazit plný text záznamu

Use of the thiol-specific derivatizing agent N-iodoacetyl-3-[125I]iodotyrosine to demonstrate conformational differences between the unbound and hsp90-bound glucocorticoid receptor hormone binding domain

Autor: L F, Stancato, A M, Silverstein, C, Gitler, B, Groner, W B, Pratt

Publikováno v: The Journal of biological chemistry. 271(15)

The hormone binding domain (HBD) of the glucocorticoid receptor (GR) contains five cysteine residues, with three of them being spaced close to one another in the steroid binding pocket. The HBD also contains the contact region for the chaperone prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::df469c0cfff85059f172774f112072fd
https://pubmed.ncbi.nlm.nih.gov/8621522

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání