Zobrazeno 1 - 10
of 17
pro vyhledávání: '"W. B. Dempsey"'
Publikováno v:
Scopus-Elsevier
Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for ch
Publikováno v:
Journal of Bacteriology. 124:686-692
A pantothenate-requiring mutant of Klebsiella pneumoniae was isolated. The mutant showed an absolute dependence on pantothenate for growth. When grown in the presence of [14C]pantothenate, the mutant incorporated [14C]pantothenate into citrate lyase
Autor:
W. B. Dempsey
Publikováno v:
Molecular Microbiology. 3:561-570
The region of the antibiotic resistance plasmid R100 that encodes the plasmid-specific transfer gene traM has two tandemly aligned promoters separated by 145 nucleotides. The principal transcripts are 705 and 562 nucleotides long. Minor transcripts a
Autor:
B E Fee, W B Dempsey
Publikováno v:
Journal of Bacteriology. 167:336-345
The fertility control gene finP, the transfer gene traM, and the transfer origin, oriT, of plasmid R100 were isolated on a single 1.2-kilobase EcoRV fragment and were then subcloned as HaeIII fragments. The sequence of the 754-base-pair finP-containi
Autor:
W B Dempsey
Publikováno v:
Journal of Bacteriology. 171:2886-2888
Conjugal transfer of the normally repressed antibiotic resistance plasmid R100 was derepressed by fragments of R100 that carried the traJ promoter and the traJ leader but lacked the finP promoter.
Autor:
W B Dempsey
Publikováno v:
Journal of bacteriology. 150(3)
After 40 min of pyridoxal starvation, pyridoxine phosphate oxidase-less mutants of Escherichia coli B derepressed pyridoxine biosynthesis 13-fold to a rate of 1.7 X 10(-9) mol/h per mg of cells. Threonine at 100 mg/liter prevented this derepression b
Autor:
S A McIntire, W B Dempsey
We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three area
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7261337818b4df54e6b912205b1ec197
https://europepmc.org/articles/PMC212476/
https://europepmc.org/articles/PMC212476/
Autor:
S Shimizu, W B Dempsey
Publikováno v:
Journal of bacteriology. 133(3)
Twenty-seven independent pyridoxineless mutants belonging to genetic linkage group I were assayed for glycolaldehyde dehydrogenase. Some mutants lacked enzyme activity entirely, and others showed activity ranging from very low to wild-type levels. Re
Autor:
W B Dempsey, N S Willetts
Single and tandem insertions of prophage lambda into R100 have been isolated. Insertions into the transfer genes, insertions into the transfer control gene finO, and insertions into regions that result in no detectable phenotypic change were found. F
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8fbad18acca0ed9ff18bf5a20211c98
https://europepmc.org/articles/PMC233271/
https://europepmc.org/articles/PMC233271/
Autor:
S Shimizu, W B Dempsey
Publikováno v:
Journal of bacteriology. 134(3)
Escherichia coli K-12 mutants with serC genotype required pyridoxine and serine for normal growth, as do E. coli B mutants of this type. Mutants of the K-12 strain, however, reverted easily to pyridoxine independence without regaining activity in the