Zobrazeno 1 - 10
of 14
pro vyhledávání: '"W W, Wilfinger"'
Publikováno v:
BioTechniques. 22(3)
The ratio of absorbance at 260 and 280 nm (the A260/280 ratio) is frequently used to assess the purity of RNA and DNA preparations. Data presented in this report demonstrate significant variability in the RNA A260/280 ratio when different sources of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7302ce4886621226f94e931ac1c76306
https://pubmed.ncbi.nlm.nih.gov/9067025
https://pubmed.ncbi.nlm.nih.gov/9067025
Publikováno v:
Space Programs and Technologies Conference and Exhibit.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::55460199befaf30fdcad0390f50abd99
https://doi.org/10.2514/6.1994-4516
https://doi.org/10.2514/6.1994-4516
Publikováno v:
Endocrinology. 98:25-32
Dispersed female rat anterior pituitary cells were cultured in Medium 199 containing 20% fetal calf serum for 30 days. Prolactin levels in the culture medium remained relatively constant during this time, ranging from 30–40 ng/10,000 cells seeded/d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aad4fd97719212668e04e198d83418d3
https://doi.org/10.1210/endo-98-1-25
https://doi.org/10.1210/endo-98-1-25
Publikováno v:
Endocrinology. 120:1503-1513
This study investigated the effects of physiological concentrations of GnRH and estradiol (E2) on LH biosynthesis and release using cultured anterior pituitary cells. Pituitaries from female rats were enzymatically dispersed and cultured for 48 h in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69ef69d91e82e429b5a95a9d9c2d3e55
https://doi.org/10.1210/endo-120-4-1503
https://doi.org/10.1210/endo-120-4-1503
Publikováno v:
Biology of reproduction. 29(1)
Anterior pituitary glands obtained from rats at various stages of the estrous cycle and from short-term ovariectomized (OVX) rats with or without physiological replacement of estradiol-17 beta (E2) and/or progesterone (P), were perifused in vitro wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4fe468c12fbac7a4be1c683070d6c62f
https://pubmed.ncbi.nlm.nih.gov/6311299
https://pubmed.ncbi.nlm.nih.gov/6311299
Publikováno v:
Endocrinology. 105(2)
The production of PRL and GH by rat pituitary cell cultures was evaluated over a 9-day period in 20 commercially available media supplemented with 5% horse serum (HS) or 5% calf serum. The quantity of PRL produced by cells maintained in a-modified Ea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc48d035e66196bb252c95bd674eb1d1
https://pubmed.ncbi.nlm.nih.gov/37069
https://pubmed.ncbi.nlm.nih.gov/37069
Publikováno v:
Cell and Tissue Research. 191
Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dbf901a5071b0dbac05a15b7a957054
https://doi.org/10.1007/bf00219803
https://doi.org/10.1007/bf00219803
Publikováno v:
Endocrinology. 120(4)
The purpose of this study was to investigate the effects of lowering the extracellular calcium concentration on GnRH-stimulated LH glycosylation and LH translation, as measured by the incorporation of [3H]glucosamine (3H-Gln) and [35S]methionine (35S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::513f0b595a27555a45142577bc0dd586
https://pubmed.ncbi.nlm.nih.gov/3549264
https://pubmed.ncbi.nlm.nih.gov/3549264
Publikováno v:
Endocrinology. 95(1)
Pituitary mammotrophs were separated by velocity sedimentation at unit gravity. Results from morphological studies showed a) that peak fractions contained prolactin-producing cells to a purity of 70% and b) that the cells retained their ultrastructur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ac7eb19217bd551b0d8aa89858182db
https://pubmed.ncbi.nlm.nih.gov/4857692
https://pubmed.ncbi.nlm.nih.gov/4857692
Autor:
Chomczynski P; Molecular Research Center, Cincinnati, OH, USA. piotr@mrcgene.com, Mackey K, Drews R, Wilfinger W
Publikováno v:
BioTechniques [Biotechniques] 1997 Mar; Vol. 22 (3), pp. 550-3.