Zobrazeno 1 - 10
of 90
pro vyhledávání: '"W Marshall Stark"'
Autor:
Gillian M. Cadden, Jan-Gero Schloetel, Grant McKenzie, Martin R. Boocock, Steven W. Magennis, W. Marshall Stark
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Serine recombinases are proposed to catalyse site-specific recombination by a unique mechanism called subunit rotation. Cutting and rejoining DNA occurs within an intermediate synaptic complex comprising a recombinase tetramer bound to two D
Externí odkaz:
https://doaj.org/article/a5af090df6ae4a95ae5b44f9a56f251c
Autor:
Alasdair I. MacDonald, Aron Baksh, Alexandria Holland, Heewhan Shin, Phoebe A. Rice, W. Marshall Stark, Femi J. Olorunniji
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)
Abstract Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integratin
Externí odkaz:
https://doaj.org/article/73b6640b58ef469a864e4d5cbc87a337
Publikováno v:
eLife, Vol 5 (2016)
Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicoba
Externí odkaz:
https://doaj.org/article/cc02f1a8843444c5a9a72ff87df08c89
Publikováno v:
PLoS ONE, Vol 6, Iss 4, p e19537 (2011)
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombin
Externí odkaz:
https://doaj.org/article/3403d058027c41e68cba14d120e5b602
Autor:
Jumai Abioye, Makeba Lawson‐Williams, Alicia Lecanda, Brecken Calhoon, Arlene L. McQue, Sean D. Colloms, W. Marshall Stark, Femi J. Olorunniji
Publikováno v:
Biotechnology journal.
Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called Serine Integrase Recombinational Assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is limite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae32c22bc7f726c6fe645871feb586a2
https://pubmed.ncbi.nlm.nih.gov/36504358
https://pubmed.ncbi.nlm.nih.gov/36504358
Autor:
Sherwin P Montaño, Sally-J Rowland, James R Fuller, Mary E Burke, Alasdair I MacDonald, Martin R Boocock, W Marshall Stark, Phoebe A Rice
Publikováno v:
Nucleic acids research.
Site-specific DNA recombinases play a variety of biological roles, often related to the dissemination of antibiotic resistance, and are also useful synthetic biology tools. The simplest site-specific recombination systems will recombine any two cogna
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a238aff985a0bf14f39d3d367d29b490
https://pubmed.ncbi.nlm.nih.gov/36100255
https://pubmed.ncbi.nlm.nih.gov/36100255
Publikováno v:
Molecular Microbiology. 114:952-965
The site-specific recombinase Tn3 resolvase initiates DNA strand exchange when two res recombination sites and six resolvase dimers interact to form a synapse. The detailed architecture of this intricate recombination machine remains unclear. We have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66fad640d81e478e9e8b1f0ce25521aa
https://doi.org/10.1111/mmi.14579
https://doi.org/10.1111/mmi.14579
Autor:
Jane E. Paget, Femi J. Olorunniji, Susan J. Rosser, Arlene L. McPherson, W. Marshall Stark, Makeba Lawson-Williams
Publikováno v:
Nucleic Acids Research
Olorunniji, F J, Lawson-Williams, M, Mcpherson, A L, Paget, J E, Stark, W M & Rosser, S J 2019, ' Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing ', Nucleic Acids Research, vol. 47, no. 21, pp. 11452-11460 . https://doi.org/10.1093/nar/gkz936
Olorunniji, F J, Lawson-Williams, M, Mcpherson, A L, Paget, J E, Stark, W M & Rosser, S J 2019, ' Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing ', Nucleic Acids Research, vol. 47, no. 21, pp. 11452-11460 . https://doi.org/10.1093/nar/gkz936
Serine integrases are emerging as core tools in synthetic biology and have applications in biotechnology and genome engineering. We have designed a split-intein serine integrase-based system with potential for regulation of site-specific recombinatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74601333b4036566283ade5d654ca48d
http://europepmc.org/articles/PMC6868429
http://europepmc.org/articles/PMC6868429
Autor:
Femi J. Olorunniji, Arlene L. McPherson, Jane E. Paget, W. Marshall Stark, Susan J. Rosser, Makeba Lawson-Williams
Serine integrases are emerging as core tools in synthetic biology and have applications in biotechnology and genome engineering. We have designed a split-intein serine integrase-based system for rapid regulation of site-specific recombination eventsi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bb206318e3b8642730abec99cabb9c5
https://doi.org/10.1101/540872
https://doi.org/10.1101/540872
Autor:
Janina Hossbach, Hong Gao, Stephanie Evans, Margaret C. M. Smith, Buvani Murugesan, W. Marshall Stark
Publikováno v:
BMC Biotechnology
BMC Biotechnology, Vol 19, Iss 1, Pp 1-10 (2019)
BMC Biotechnology, Vol 19, Iss 1, Pp 1-10 (2019)
Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify integrating vectors, derived using phageint
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03610d81ac1d6bffc67d5195ec669f6b
https://pubmed.ncbi.nlm.nih.gov/31164159
https://pubmed.ncbi.nlm.nih.gov/31164159