Zobrazeno 1 - 10
of 13
pro vyhledávání: '"W H, Velander"'
Publikováno v:
Journal of Assisted Reproduction and Genetics. 20:148-152
Purpose: The objective was to establish the parameters for reversible electroporation of murine embryos. Methods: In Trial 1, murine presumptive zygotes received an electrical pulse of 5, 10, or 20-μs duration, and one of five voltages (100, 200, 25
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6bbc625f189071a4174bfd4316ec16c6
https://doi.org/10.1023/a:1022929119792
https://doi.org/10.1023/a:1022929119792
Autor:
B. L. Williams, A.E.T. Sparks, R. S. Canseco, J. W. Knight, J. L. Johnson, W. H. Velander, R. L. Page, W. N. Drohan, J. M. Young, R. E. Pearson, T. D. Wilkins, F. C. Gwazdauskas
Publikováno v:
Journal of Animal Science. 70:2207-2211
The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28d2f516b44645dd3394620e82bca1fc
https://doi.org/10.2527/1992.7072207x
https://doi.org/10.2527/1992.7072207x
Publikováno v:
Transgenic research. 10(1)
Colostrum and milk are natural vehicles for acquiring passive immunity and are valuable tools for decreasing neonatant mortality from diarrheal disease. The effects of recombinant human protein C (rhPC) expression levels on endogenous immunoglobulin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9a1ccb93d003dffc3649531c3fd20d7e
https://pubmed.ncbi.nlm.nih.gov/11252382
https://pubmed.ncbi.nlm.nih.gov/11252382
Current progress in the production of recombinant human fibrinogen in the milk of transgenic animals
Publikováno v:
Thrombosis and haemostasis. 78(1)
The mammary gland of transgenic animals has several advantages for production of heterologous proteins including a high cell density that results in high concentrations of secreted protein. While the mammary gland appears to be able to secrete fully
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d965ec498b24faa269244cdad59e75c6
https://pubmed.ncbi.nlm.nih.gov/9198211
https://pubmed.ncbi.nlm.nih.gov/9198211
Autor:
K E, Van Cott, H, Lubon, C G, Russell, S P, Butler, F C, Gwazdauskas, J, Knight, W N, Drohan, W H, Velander
Publikováno v:
Transgenic research. 6(3)
The genotypic and phenotypic stability of four lines of transgenic pigs expressing recombinant human protein C in milk was examined. Two lines were established with a construct consisting of a 2.6 kb mouse WAP promoter and a 9.4 kb human protein C ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cb277be2d9bd30d1529536ab1b174917
https://pubmed.ncbi.nlm.nih.gov/9167268
https://pubmed.ncbi.nlm.nih.gov/9167268
Autor:
R. K. Paleyanda, W. H. Velander, T. K. Lee, R. Drews, F. C. Gwazdauskas, J. W. Knight, W. N. Drohan, H. Lubon
Publikováno v:
ACS Symposium Series ISBN: 9780841234550
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::468f19305a370a6684038f6ec6ce0bfd
https://doi.org/10.1021/bk-1996-0647.ch014
https://doi.org/10.1021/bk-1996-0647.ch014
Autor:
A, Subramanian, W H, Velander
Publikováno v:
Journal of molecular recognition : JMR. 9(5-6)
The impact of antibody orientation on immunosorbent efficiency was quantitatively assessed. A pH-dependent murine monoclonal antibody (Mab) against human protein C (hPC), recombinant hPC (rhPC) and two different immobilization chemistries and matrice
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::02a10193df0f90b6806efc8b8020d5d8
https://pubmed.ncbi.nlm.nih.gov/9174936
https://pubmed.ncbi.nlm.nih.gov/9174936
Publikováno v:
Journal of molecular recognition : JMR. 9(5-6)
Recombinant human protein C (rhPC) secreted in the milk of transgenic pigs was studied. Transgenes having different regulatory elements of the murine milk protein, whey acidic protein, were used with cDNA and genomic human protein C (hPC) DNA sequenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6fb962846e0193fe75f38c11e53de7f2
https://pubmed.ncbi.nlm.nih.gov/9174918
https://pubmed.ncbi.nlm.nih.gov/9174918
Autor:
M A, Hajdu, J W, Knight, R S, Canseco, R L, Krisher, W H, Velander, R E, Pearson, F C, Gwazdauskas
Publikováno v:
Journal of animal science. 72(5)
A series of experiments evaluated development of porcine zygotes microinjected with DNA in three culture media and two incubation temperatures, from postpubertal and prepubertal donors, and between zygotes injected with DNA into the pronucleus and th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b49bccc625020de296ec5080749d2940
https://pubmed.ncbi.nlm.nih.gov/8056677
https://pubmed.ncbi.nlm.nih.gov/8056677
Publikováno v:
Advances in Bioprocess Engineering ISBN: 9789048144594
Transgenic pigs which produced from 0.1 to 1.0 mg m1−1 of recombinant human Protein C (rHPC) in milk were generated. A process for the purification of rHPC from milk has been developed which used selective precipitation by polyethylene glycol (PEG)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a79656b73bbe3071c2dd50f6e54767b
https://doi.org/10.1007/978-94-017-0641-4_70
https://doi.org/10.1007/978-94-017-0641-4_70