Zobrazeno 1 - 10
of 14
pro vyhledávání: '"W E Borrias"'
The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). The gene is transcribed into a 1250-base transcript. The expression of the flavodoxin gene was analysed and found to be regulated at the tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e55fe9e76a3b80f9fbf251cd3e50641
https://europepmc.org/articles/PMC1130618/
https://europepmc.org/articles/PMC1130618/
Autor:
C.A.M.J.J. van den Hondel, W. E. Borrias, R.W. van Leen, Adri A. M. Thomas, C.J. Kuhlemeier, A van der Ende, G.A. van Arkel
Publikováno v:
Plasmid, 10(2), 156-163. Academic Press Inc.
We describe the construction of a series of vectors suitable for gene cloning in the cyanobacterium Anacystis nidulans R2. From the indigenous plasmid pUH24, derivatives were constructed with streptomycin as the selective marker; one of these plasmid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60690f87c042f20fa3252207e5d90a26
https://doi.org/10.1016/0147-619x(83)90068-9
https://doi.org/10.1016/0147-619x(83)90068-9
Publikováno v:
Molecular and General Genetics MGG. 213:223-228
Eighteen mutant strains of the unicellular cyanobacterium Anacystis nidulans R2 that are unable to assimilate nitrate have been isolated after transposon Tn901 mutagenesis. Characterization of phenotypes and transformation tests have allowed the dist
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::51ec2ea421eefec0d969fbef8bf4d1a1
https://doi.org/10.1007/bf00339585
https://doi.org/10.1007/bf00339585
Publikováno v:
Molecular and General Genetics MGG. 105:152-163
Complementation experiments with temperature sensitive (ts) and suppressor sensitive (sus) mutants of bacteriophage ϕX174 unambiguously revealed five cistrons on the basis of a clear bipartition of burst sizes.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95095afdb825b8fecaf658a889d2dbdf
https://doi.org/10.1007/bf00445685
https://doi.org/10.1007/bf00445685
Mutants of bacteriophage G4 were isolated and characterized, and their mutations were mapped. They constitute six different genes, namely, A, B, E, F, G, and H. The functional relationship with bacteriophage phi X174 was determined by complementation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e173ddb681c2740f4b9056a6ea19891
https://europepmc.org/articles/PMC353451/
https://europepmc.org/articles/PMC353451/
Autor:
W. E. Borrias, C.A.M.J.J. van den Hondel, G.A. van Arkel, Peter Weisbeek, S Verbeek, A van der Ende
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 77(3), 1570-1574. National Academy of Sciences
We have used the TEM beta-lactamase transposon Tn901, located on Escherichia coli plasmid pRI46, to introduce in vivo a genetic marker into plasmid pUH24, present in the cyanobacterial strain Anacystis nidulans R-2. Restriction enzyme analysis and he
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4dd04f1485d6a06b6966c71e17667cd
https://pure.amc.nl/en/publications/introduction-of-transposon-tn901-into-a-plasmid-of-anacystis-nidulans-preparation-for-cloning-in-cyanobacteria(2ed9a25c-85e0-48cd-af1c-65bf53f82e10).html
https://pure.amc.nl/en/publications/introduction-of-transposon-tn901-into-a-plasmid-of-anacystis-nidulans-preparation-for-cloning-in-cyanobacteria(2ed9a25c-85e0-48cd-af1c-65bf53f82e10).html
Publikováno v:
Moleculargeneral genetics : MGG. 184(2)
Two plasmids were constructed consisting of the E. coli vector pACYC184 and the cyanobacterial plasmid pUC1. These recombinants, designated pUC104 and pUC105, can be transformed to E. coli K12 as well as to the cyanobacterium Anacystis nidulans R2 an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b24017939dc1f0d740284179dff47b5
https://pubmed.ncbi.nlm.nih.gov/6276694
https://pubmed.ncbi.nlm.nih.gov/6276694
Autor:
N, Tandeau de Marsac, W E, Borrias, C J, Kuhlemeier, A M, Castets, G A, van Arkel, C A, van den Hondel
Publikováno v:
Gene. 20(1)
A new strategy for molecular cloning in the cyanobacterium Anacystis nidulans R-2 is described. This strategy involved the use of a transposon and was developed for the cloning of a gene encoding methionine biosynthesis. A met::Tn901 mutant was isola
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8bc16250bb18dab902bbcb0a8aeff4d2
https://pubmed.ncbi.nlm.nih.gov/6298064
https://pubmed.ncbi.nlm.nih.gov/6298064
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 74(6)
The map position of several phiX174 mutations in the genes A and B was determined by marker rescue with DNA fragments produced by the restriction enzymes Hha I, HindII, Hae III, and Alu I. All the gene B mutants were found to be located within gene A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdd78a7afdc89f3f3db54b7152c63aa6
https://pubmed.ncbi.nlm.nih.gov/267943
https://pubmed.ncbi.nlm.nih.gov/267943
Publikováno v:
Nature. 261(5557)
GENE A of the single-stranded DNA bacteriophage φX174 has a key position in the replication of the first double-stranded replicative form (parental RF) to progeny RF DNA molecules. The action of gene A manifests itself in the introduction of a nick
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::024603454fabc6f78574632957328fed
https://pubmed.ncbi.nlm.nih.gov/1272397
https://pubmed.ncbi.nlm.nih.gov/1272397