Výsledky vyhledávání

Effect of flaxseed oil, animal fat, and vitamin E supplementation on growth performance, serum metabolites, and carcass characteristics of finisher pigs, and physical characteristics of pork

Autor: W. E. Magee, Y. Wang, Christy L. Bratcher, C. Huang, D. A. Griffing, L. I. Chiba, Werner G. Bergen, I.M. Torres, E.A. Spangler, Soren P. Rodning

Publikováno v: Livestock Science. 220:143-151

Dietary lipid supplementation can reduce de novo lipogenesis and increase intramuscular fat content of pork simultaneously, and vitamin E supplementation can increase the oxidative stability of pork. A total of 96 pigs (Yorkshire) were used to invest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ca1c09bdacb44019207616e7fcebca96
https://doi.org/10.1016/j.livsci.2018.11.011

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Effect of flaxseed oil, poultry fat, and vitamin E supplementation on physical and organoleptic characteristics and fatty acid profile of pork, and expression of genes associated with lipid metabolism

Autor: E.A. Spangler, Y. Wang, Christy L. Bratcher, Soren P. Rodning, W. E. Magee, L. I. Chiba, C. Huang, Werner G. Bergen

Publikováno v: Livestock Science. 231:103849

A total of 96 pigs (Yorkshire; 54.3 ± 3.4 kg body weight) were used to investigate the effect of dietary lipid (0 or 1% flaxseed oil + 1, 3, or 5% poultry fat for the diets supplemented with lipids) and vitamin E (11 or 220 IU vitamin E/kg) suppleme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f6e0c52ee76ab21db3c833c58c47b1be
https://doi.org/10.1016/j.livsci.2019.103849

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An antibody adsorption technique facilitates antigen selection for development of serotype-specific monoclonal antibodies to Yersinia enterocolitica

Autor: Q, Li, W E, Magee

Publikováno v: BioTechniques. 14(6)

A simple procedure is described to aid in the selection of antigens from gram-negative organisms that are most likely to exhibit serotype-specific epitopes when used as immunogens in the mouse. Several such proteins of Yersinia enterocolitica serotyp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5c4628ffe23a565aea976d8c38c53b8f
https://pubmed.ncbi.nlm.nih.gov/8333964

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Effect of various glucose/glutamine ratios on hybridoma growth, viability and monoclonal antibody formation

Autor: Y. T. Luan, R. Mutharasan, W. E. Magee

Publikováno v: Biotechnology Letters. 9:535-538

Cultivation of a model hybridoma cell line in Dulbecco's modification of Eagle's minimal essential medium (DMEM) at various glucose and glutamine levels showed that maxmium cell growth was obtained in a broad range of glucose/glutamine ratios of 0.6

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e889b9e185f342dbf32840cc33d92d5f
https://doi.org/10.1007/bf01026656

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A novel immobilized hybridoma reactor for the production of monoclonal antibodies

Autor: Octavio T. Ramírez, W. E. Magee, R. Mutharasan

Publikováno v: Biotechnology Techniques. 1:245-250

Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical glass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f64763d2cf19e76c61289b0c8e46b36e
https://doi.org/10.1007/bf00155463

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Strategies to extend longevity of hybridomas in culture and promote yield of monoclonal antibodies

Autor: W. E. Magee, R. Mutharasan, Y. T. Luan

Publikováno v: Biotechnology Letters. 9:691-696

Cell viability was improved by supplemental feeding of amino acids and vitamins in batch culture of hybridoma cells. Cells could be maintained over a 10 day period following exponential growth at a constant viable cell concentration of 2.1×106 cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b45bc8cf1e654be799496afeba7f421e
https://doi.org/10.1007/bf01024599

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